Sanger Institute, Genome Research Limited

People

Ling Ng, Bee
Bee Ling Ng
Group Leader

The Cytometry Core Facility team is made up of 3 staff members. All of our team members have many years of experience in different aspects of flow cytometry. We dedicate our time and efforts in helping and assisting our researchers in their flow cytometry experiments. The facility collaborates with various research projects using flow cytometric techniques to isolate and purify cells and chromosomes for structural and functional studies, sequencing, microarrays as well as fluorescence insitu hybridisation (FISH) experiment.

 

 

 

Publications

  • A high-throughput in vivo micronucleus assay for genome instability screening in mice.

    Balmus G, Karp NA, Ng BL, Jackson SP, Adams DJ and McIntyre RE

    Nature protocols 2015;10;1;205-15

  • Genome sequencing and analysis of the Tasmanian devil and its transmissible cancer.

    Murchison EP, Schulz-Trieglaff OB, Ning Z, Alexandrov LB, Bauer MJ et al.

    Cell 2012;148;4;780-91

  • Laser excitation power and the flow cytometric resolution of complex karyotypes.

    Ng BL and Carter NP

    Cytometry. Part A : the journal of the International Society for Analytical Cytology 2010;77;6;585-8

  • Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays.

    Gribble SM, Ng BL, Prigmore E, Fitzgerald T and Carter NP

    Nature protocols 2009;4;12;1722-36

  • Flow analysis and sorting of microchromosomes (<3 Mb).

    Ng BL, Yang F and Carter NP

    Cytometry. Part A : the journal of the International Society for Analytical Cytology 2007;71;6;410-3

  • Factors affecting flow karyotype resolution.

    Ng BL and Carter NP

    Cytometry. Part A : the journal of the International Society for Analytical Cytology 2006;69;9;1028-36

  • Evolutionary dynamics of Anolis sex chromosomes revealed by sequencing of flow sorting-derived microchromosome-specific DNA.

    Kichigin IG, Giovannotti M, Makunin AI, Ng BL, Kabilov MR et al.

    Molecular genetics and genomics : MGG 2016;291;5;1955-66

  • New insights into sex chromosome evolution in anole lizards (Reptilia, Dactyloidae).

    Giovannotti M, Trifonov VA, Paoletti A, Kichigin IG, O'Brien PC et al.

    Chromosoma 2016

  • The pig X and Y Chromosomes: structure, sequence, and evolution.

    Skinner BM, Sargent CA, Churcher C, Hunt T, Herrero J et al.

    Genome research 2016;26;1;130-9

  • Identification of disrupted AUTS2 and EPHA6 genes by array painting in a patient carrying a de novo balanced translocation t(3;7) with intellectual disability and neurodevelopment disorder.

    Schneider A, Puechberty J, Ng BL, Coubes C, Gatinois V et al.

    American journal of medical genetics. Part A 2015;167A;12;3031-7

  • A First Generation Comparative Chromosome Map between Guinea Pig (Cavia porcellus) and Humans.

    Romanenko SA, Perelman PL, Trifonov VA, Serdyukova NA, Li T et al.

    PloS one 2015;10;5;e0127937

  • A high-throughput in vivo micronucleus assay for genome instability screening in mice.

    Balmus G, Karp NA, Ng BL, Jackson SP, Adams DJ and McIntyre RE

    Nature protocols 2015;10;1;205-15

  • Chromosomal evolution among leaf-nosed nectarivorous bats--evidence from cross-species chromosome painting (Phyllostomidae, Chiroptera).

    Sotero-Caio CG, Volleth M, Gollahon LS, Fu B, Cheng W et al.

    BMC evolutionary biology 2013;13;276

  • Genetic basis of Y-linked hearing impairment.

    Wang Q, Xue Y, Zhang Y, Long Q, Asan et al.

    American journal of human genetics 2013;92;2;301-6

  • Massively parallel sequencing reveals the complex structure of an irradiated human chromosome on a mouse background in the Tc1 model of Down syndrome.

    Gribble SM, Wiseman FK, Clayton S, Prigmore E, Langley E et al.

    PloS one 2013;8;4;e60482

  • Genome sequencing and analysis of the Tasmanian devil and its transmissible cancer.

    Murchison EP, Schulz-Trieglaff OB, Ning Z, Alexandrov LB, Bauer MJ et al.

    Cell 2012;148;4;780-91

  • Reprogramming of T cells to natural killer-like cells upon Bcl11b deletion.

    Li P, Burke S, Wang J, Chen X, Ortiz M et al.

    Science (New York, N.Y.) 2010;329;5987;85-9

  • Laser excitation power and the flow cytometric resolution of complex karyotypes.

    Ng BL and Carter NP

    Cytometry. Part A : the journal of the International Society for Analytical Cytology 2010;77;6;585-8

  • De novo apparently balanced translocations in man are predominantly paternal in origin and associated with a significant increase in paternal age.

    Thomas NS, Morris JK, Baptista J, Ng BL, Crolla JA and Jacobs PA

    Journal of medical genetics 2010;47;2;112-5

  • Separation of the PROX1 gene from upstream conserved elements in a complex inversion/translocation patient with hypoplastic left heart.

    Gill HK, Parsons SR, Spalluto C, Davies AF, Knorz VJ et al.

    European journal of human genetics : EJHG 2009;17;11;1423-31

  • Human Y chromosome base-substitution mutation rate measured by direct sequencing in a deep-rooting pedigree.

    Xue Y, Wang Q, Long Q, Ng BL, Swerdlow H et al.

    Current biology : CB 2009;19;17;1453-7

  • Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays.

    Gribble SM, Ng BL, Prigmore E, Fitzgerald T and Carter NP

    Nature protocols 2009;4;12;1722-36

  • Accurate whole human genome sequencing using reversible terminator chemistry.

    Bentley DR, Balasubramanian S, Swerdlow HP, Smith GP, Milton J et al.

    Nature 2008;456;7218;53-9

  • Definition of the zebrafish genome using flow cytometry and cytogenetic mapping.

    Freeman JL, Adeniyi A, Banerjee R, Dallaire S, Maguire SF et al.

    BMC genomics 2007;8;195

  • Flow analysis and sorting of microchromosomes (<3 Mb).

    Ng BL, Yang F and Carter NP

    Cytometry. Part A : the journal of the International Society for Analytical Cytology 2007;71;6;410-3

  • Factors affecting flow karyotype resolution.

    Ng BL and Carter NP

    Cytometry. Part A : the journal of the International Society for Analytical Cytology 2006;69;9;1028-36

  • Expression of mammalian GPCRs in C. elegans generates novel behavioural responses to human ligands.

    Teng MS, Dekkers MP, Ng BL, Rademakers S, Jansen G et al.

    BMC biology 2006;4;22

  • Micro-array analyses decipher exceptional complex familial chromosomal rearrangement.

    Fauth C, Gribble SM, Porter KM, Codina-Pascual M, Ng BL et al.

    Human genetics 2006;119;1-2;145-53

  • BRCTx is a novel, highly conserved RAD18-interacting protein.

    Adams DJ, van der Weyden L, Gergely FV, Arends MJ, Ng BL et al.

    Molecular and cellular biology 2005;25;2;779-88

  • Applications of combined DNA microarray and chromosome sorting technologies.

    Gribble SM, Fiegler H, Burford DC, Prigmore E, Yang F et al.

    Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 2004;12;1;35-43

  • Chromosome paints from single copies of chromosomes.

    Gribble S, Ng BL, Prigmore E, Burford DC and Carter NP

    Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 2004;12;2;143-51