Members of this family have a linear single stranded, positive sense RNA genome of 7.1 to 8.9 kilobases, which is polyadenylated. Their genomes consist of a single open-reading frame, which encodes a polyprotein. This is then cleaved co- and post- translationally by viral proteases to give the viral structural and non-structural proteins.
There are 12 genera in the Picornaviridae: Enterovirus, Cardiovirus, Aphthovirus, Hepatovirus, Parechovirus, Erbovirus, Kobuvirus, Teschovirus, Sapelovirus, Senecavirus, Tremovirus and Avihepatovirus.
We are currently involved in a project to sequence isolates of Human echovirus 30, a member of the Enterovirus genus, which is associated with aseptic meningitis and encephalitis.
Species: Human enterovirus B
Non-enveloped RNA virus, with a positive-sense RNA genome, which consists of approximately 7,500 nucleotides. This encodes a polyprotein which is cleaved to produce structural (capsid proteins VP1 to VP4) and nonstructural (2A to 3D) proteins. There are 36 serotypes of Human enterovirus B – Human coxsackievirus A9, Human coxsackievirus B1–B6, Human echovirus 1–7, 9, 11-21, 24-27, 29-33, Human enterovirus 69. Enteroviruses can readily infect humans and are spread by oral/faecal or respiratory routes.
Human Echovirus 30 – Associated with aseptic meningitis and encephalitis, although infection in immunocompetent individuals is often asymptomatic.
In collaboration with Professor Peter Simmonds and Dr Carol Leitch (Centre for Infectious Diseases, University of Edinburgh) we are using Roche/454 technology to sequence the whole genomes of a group of Human echovirus 30 isolates. This project is funded by the Wellcome Trust.
Published Genome Data
Human echovirus 30 isolate 14916net87, complete genome, DQ534205 can be found here.
This project is ongoing and data for this organism will be made available in due course.
Data Use Statement
This sequencing centre plans on publishing the completed and annotated sequences in a peer-reviewed journal as soon as possible. Permission of the principal investigator should be obtained before publishing analyses of the sequence/open reading frames/genes on a chromosome or genome scale. See our data sharing policy.
Please address all sequencing enquiries to: firstname.lastname@example.org