T. congolense is a major livestock pathogen in Africa. Despite the severe disease it causes, T. congolense remains largely unstudied. The Sanger Institute's Pathogen Sequencing Unit (PSU), in collaboration with Professor David Barry (Glasgow University, UK), Dr Alberto Davila (Instituto Oswalso Cruz, Brazil) and Dr Phelix Majiwa (International Livestock Institute, Kenya), is carrying out a partial genome shotgun of this parasite, initially to a 1x coverage.
The Wellcome Sanger Institute Pathogen Genomics group has partially shotgun sequenced the nuclear genome of the livestock-infective Trypanosoma congolense. This will serve as a useful comparative genomics resource to complement the genomes of Trypanosoma vivax and Trypanosoma brucei. The T. congolense and T. vivax partial shotgun projects are being carried out in collaboration with David Barry (Glasgow University, UK), Alberto Davila (Instituto Oswalso Cruz, Brazil), Phelix Majiwa (International Livestock Institute, Kenya) and Sara Melville (University of Cambridge, UK).
Published Genome Data
The strain chosen for sequencing was clone IL3000 (Bienen et al. 1991), isolated from a cow in the Transmara. It is capable of growth in laboratory rodents, is fly transmissible and is able to grow in vitro.
For this partial genome shotgun pilot project, nuclear DNA was isolated at ILRI from bloodstream stage T. congolense raised in rats. Small insert libararies (2-4kb) were constructed by cloning sheared DNA into pUC18 vectors. The targeted shotgun coverage is 5x.
The Trypanosoma congolense EST project
The Wellcome Sanger Institute Pathogen Genomics group has sequenced ESTs prepared from 6 different T. congolense libraries in collaboration with John Donelson (University of Iowa). To date, ca. 4500 ESTs from each of the libraries have been sequenced, clustered and analysed for similarity.
These libraries were
- Bloodstream stage library
- Bloodstream stage normalised library
- Epimastigote stage library
- Metacyclic stage library
- Metacyclic stage normalised library
- Procyclic stage library
Data Use Statement
This sequencing centre plans on publishing the completed and annotated sequences in a peer-reviewed journal as soon as possible. Permission of the principal investigator should be obtained before publishing analyses of the sequence/open reading frames/genes on a chromosome or genome scale. See our data sharing policy.
Please address all sequencing enquiries to: firstname.lastname@example.org