Plasmodium berghei

P. berghei is a murine malaria species, originally isolated from thicket rats in Central Africa. The species is one of four common murine model but its relative ease of transfection has made it a particularly popular model for reverse genetics. Although the ‘core’ genome shares large block of synteny with human malaria parasites, there are several well-characterised breaks in synteny and the gene organisation in the subtelomeres is substantially different.

Data Downloads

[Genome Research Limited]

An initial analysis of a low-coverage (3x) draft genome produced by Sanger-sequencing was published by Hall et al. in 2005. From these data, P. berghei has an estimated genome size of 18-20 Mb, with 14 chromosomes in the size range of 0.6 Mb to 3.8 Mb. DNA for this sequencing project was obtained from asynchronous bloodstages of clone 15cy1 of the ANKA strain of P. berghei.

Further sequencing of P. berghei ANKA and several other strains has been completed using Illumina Sequence-by-synthesis technology. A publication describing the comprehensive re-assembly and annotation is underway. Please refer to Data Use Statement.

Studies

PlasmoGEM: Towards an open -access, genome-scale Plasmodium genetic modification resource

Sample

Strain

Run Accession

23-Rec43Col3UnknownERR411593
22-Rec43Col2UnknownERR411592
21-Rec43Col1UnknownERR411591
20-Rec42Col4UnknownERR411590
19-Rec42Col3UnknownERR411589
18-Rec42Col2UnknownERR411588
17-Rec42Col1UnknownERR411587
36-Km8SCUnknownERR411586
35-ArgSCUnknownERR411585
34-ArgSCUnknownERR411584
33-ArgSCUnknownERR411583
32-PbAC4bUnknownERR411582
31-TechSeqFO_NonOgroup2UnknownERR411581
30-TechSeqFO_NonOgroup1UnknownERR411580
29-Rec39WTClonalUnknownERR411579
28-Rec39WTCol1_DILCol2UnknownERR411578
27-Rec39WTCol1_DILCol1UnknownERR411577
26-Rec39GWCol1_DILCol2UnknownERR411576
25-Rec39GWCol1_DILCol1UnknownERR411575
24-Rec43Col4UnknownERR411574
16-Rec41Col4UnknownERR403744
15-Rec41Col3UnknownERR403743
14-Rec41Col2UnknownERR403742
13-Rec41Col1UnknownERR403741
12-Rec40Col4UnknownERR403740
11-Rec40Col3UnknownERR403739
10-Rec40Col2UnknownERR403738
9-Rec40Col1UnknownERR403737
8-Rec39Col4UnknownERR403736
7-Rec39Col3UnknownERR403735
6-Rec39Col2UnknownERR403734
5-Rec39Col1UnknownERR403733
4-Rec38Col4UnknownERR403732
3-Rec38Col3UnknownERR403731
2-Rec38Col2UnknownERR403730
1-Rec38Col1UnknownERR403729
arg475UnknownERR403728
arg474UnknownERR403727
arg473UnknownERR403726
arg472UnknownERR403725
pJPbPACUnknownERR314299
DHHC_TAG_WTA_L-RhamUnknownERR314298
DHH_KO_WTAC_L-ArabUnknownERR314297
ARG_KinasesUnknownERR314296
Rec12_Ap2_G-H_Rec21_H4UnknownERR314295
Rec33_Ap2_C-D_Rec21_H3UnknownERR314294
Rec30_Ap2_A-B_Rec21_H2UnknownERR314293
Rec29_Rec21_H1UnknownERR314292
Rec28_Rec21_G12UnknownERR314291
Rec27_Rec21_G11_AP2G3_COMPUnknownERR314290
Rec36_wtUnknownERR311253
BADgbaADIL2UnknownERR311252
BADgbaADIL1UnknownERR311251
MockWTGW2UnknownERR311250
MockWTGW1UnknownERR311249
MockWTZeo2UnknownERR311248
MockWTZeo1UnknownERR311247
RhaBgbaAGW2UnknownERR311246
RhaBgbaAGW1UnknownERR311245
RhaBgbaAZeo2UnknownERR311244
RhaBgbaAZeo1UnknownERR311243
BADgbaAGW2UnknownERR311242
BADgbaAGW1UnknownERR311241
BADgbaAZeo2UnknownERR311240
BADgbaAZeo1UnknownERR311239
2358STDY5461625UnknownERR279200
2358STDY5461624UnknownERR279199
2358STDY5461623UnknownERR279198
2358STDY5461622UnknownERR279197
2358STDY5461621UnknownERR279196
2358STDY5461620UnknownERR279195
2358STDY5461619UnknownERR279194
2358STDY5461618UnknownERR279193
Rec00016TAG_pool6UnknownERR311238
Rec000015KO_pool5UnknownERR311237
Rec000021TAG_Rec000022KO_pool4UnknownERR311236
Rec000021KO_Rec000022TAG_pool3UnknownERR311235
Rec000019_20TAG_pool2UnknownERR311234
Rec000019_20KO_pool1UnknownERR311233
Rec17-18-AP2_pool7UnknownERR311232
Rec0001Orig96x_pool6UnknownERR311231
RecARGKIN_pool5UnknownERR311230
Rec000010-11TAG_pool4UnknownERR311229
Rec000010-11KO_pool3UnknownERR311228
Rec000012KO_pool2UnknownERR311227
Rec000013TAG_pool1UnknownERR311226
Rec000018_gw_pool3UnknownERR200605
Rec000017_gw_pool2UnknownERR200604
Rec000017_wt_pool1UnknownERR200603
12-Rec48Col4UnknownERR467261
11-Rec48Col3UnknownERR467260
10-Rec48Col2UnknownERR467259
9-Rec48Col1UnknownERR467258
8-Rec45Col4UnknownERR467257
7-Rec45Col3UnknownERR467256
6-Rec45Col2UnknownERR467255
5-Rec45Col1UnknownERR467254
4-Rec44Col4UnknownERR467253
3-Rec44Col3UnknownERR467252
2-Rec44Col2UnknownERR467251
1-Rec44Col1UnknownERR467250
22-TechSeqFO_NonOgroup4UnknownERR467249
21-TechSeqFO_NonOgroup3UnknownERR467248
20-Rec36Col4UnknownERR467247
19-Rec36Col3UnknownERR467246
18-Rec36Col2UnknownERR467245
17-Rec36Col1UnknownERR467244
16-Rec49Col4UnknownERR467243
15-Rec49Col3UnknownERR467242
14-Rec49Col2UnknownERR467241
13-Rec49Col1UnknownERR467240
19-PKG-HA_MathieuUnknownERR473430
18-Rec39_NonOgroup2_Col2UnknownERR473429
17-Rec39_NonOgroup2_Col1UnknownERR473428
16-Rec38_NonOgroup2_Col4UnknownERR473427
15-Rec38_NonOgroup2_Col3UnknownERR473426
14-Rec38_NonOgroup2_Col2UnknownERR473425
13-Rec38_NonOgroup2_Col1UnknownERR473424
12-Rec38_NonOgroup1_col4UnknownERR473423
11-Rec38_NonOgroup1_col3UnknownERR473422
10-Rec38_NonOgroup1_col2UnknownERR473421
9-Re38_NonOgroup1_col1UnknownERR473420
8-Rec47_NonOgroup1_col4UnknownERR473419
7-Rec47_NonOgroup_col3UnknownERR473418
6-Rec47_NonOgroup1_col2UnknownERR473417
5-Rec47_NonOgroup1_col1UnknownERR473416
4-Rec46_NonOgroup1_col4UnknownERR473415
3-Rec46_NonOgroup1_col3UnknownERR473414
2-Rec46_NonOgroup1_col2UnknownERR473413
1-Rec46_NonOgroup1_col1UnknownERR473412
qc1379UnknownERR536860
qc1378UnknownERR536859
qc1377UnknownERR536858
qc1376UnknownERR536857
qc1375UnknownERR536856
qc1374UnknownERR536855
qc1373UnknownERR536854
qc1372UnknownERR536853
qc1371UnknownERR536852
qc1370UnknownERR536851
qc1369UnknownERR536850
qc1368UnknownERR536849
qc1306UnknownERR536848
qc1305UnknownERR536847
qc1304UnknownERR536846
qc1303UnknownERR536845
qc1302UnknownERR536844
qc1301UnknownERR536843
qc1300UnknownERR536842
qc1299UnknownERR536841
qc1298UnknownERR536840
qc1297UnknownERR536839
qc1296UnknownERR536838
qc1295UnknownERR536837
Pf_final2UnknownERR558618
Pf_final1UnknownERR558617
qc1446UnknownERR558616
qc1445UnknownERR558615
qc1444UnknownERR558614
qc1443UnknownERR558613
qc1438UnknownERR558612
qc1437UnknownERR558611
qc1436UnknownERR558610
qc1435UnknownERR558609
qc1432UnknownERR558608
qc1431UnknownERR558607
qc1430UnknownERR558606
qc1429UnknownERR558605
qc1428UnknownERR558604
qc1427UnknownERR558603
qc1426UnknownERR558602
qc1425UnknownERR558601
qc1424UnknownERR558600
qc1423UnknownERR558599
qc1422UnknownERR558598
qc1421UnknownERR558597
  • A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analyses.

    Hall N, Karras M, Raine JD, Carlton JM, Kooij TW, Berriman M, Florens L, Janssen CS, Pain A, Christophides GK, James K, Rutherford K, Harris B, Harris D, Churcher C, Quail MA, Ormond D, Doggett J, Trueman HE, Mendoza J, Bidwell SL, Rajandream MA, Carucci DJ, Yates JR, Kafatos FC, Janse CJ, Barrell B, Turner CM, Waters AP and Sinden RE

    Science (New York, N.Y.) 2005;307;5706;82-6

Data Use Statement

This sequencing centre plans on publishing the completed and annotated sequences in a peer-reviewed journal as soon as possible. Permission of the principal investigator should be obtained before publishing analyses of the sequence/open reading frames/genes on a chromosome or genome scale. See our data sharing policy.

Sequencing enquiries

Please address all sequencing enquiries to: pathinfo@sanger.ac.uk

* quick link - http://q.sanger.ac.uk/zbopyu2b