Plasmid genomes - data download

This page provides access to genomes sequenced at the Wellcome Trust Sanger Institute.

We make all our sequence data available through the links below. The below list may include complete, ongoing and forthcoming sequencing projects.

[Genome Research Limited]

The Sanger Institute Pathogen Genomics group is sequencing a number of plasmids from various sources. Sequence data for each project will be made available for download (FTP site) and searching (BLAST server) once assemblies have begun. The annotation will be made available upon publication.

Plasmid Information

Plasmid

Source

Size

Phenotype

Status

Data

pBToxis* Bacillus thuringiensis 137 kb insecticidal toxins Published FTP site
EMBL/GenBank
BLAST server
pCoo** Enterotoxigenic E. coli 98.4 kb CS1 pilus Published FTP site
EMBL/GenBank
BLAST server
R478*** Serratia marcescens 286kb Antibiotic resistance Published FTP site
EMBL/GenBank
BLAST server
pHag1/pHag2 Bacteroides fragilis strain WI1 5.6 kb/9.9 kb Antibiotic resistance Finished FTP site

* In collaboration with Dr. Colin Berry of the School of Biosciences, Cardiff University and Dr Arieh Zaritsky of the Department of Life Sciences, Ben-Gurion University of the Negev, Israel.
** In collaboration with June Scott of the Dept of Microbiology and Immunology, Emory University, USA.
*** In collaboration with Diane Taylor of the Department of Medical Microbiology and Immunology, University of Alberta, Canada.

In collaboration with Christopher Thomas (University of Birmingham), John Wain (Sanger Institute) and the broader plasmid biology community (with advisors Anne Summers, Athens Georgia, USA and Ron Skurray, Sydney, Australia) we are sequencing reference plasmids for each of the Escherichia coli incompatibility groups for which no complete sequence exists, along with equivalent plasmids from modern clinical isolates. These are all listed below. If you have a plasmid that might be interesting to sequence in this context please contact Christopher Thomas. The sequencing of plasmid pVL501 is in collaboration with Jonathan Fletcher (University of Bradford).

Plasmid Information

Plasmid

Size

Inc group

NCTC#

Resistances

Status

Data

RA1 129 kb IncA/C 50073 Su Tc Planned -
R667 168 kb IncA/C 50463 Ap Su Finished Sequence
TP113 86 kb IncB=O 50084 Km Hg Finished Sequence
TP114 62 kb IncIbeta(2) 50085 Km Planned -
R387 80 kb IncK 50022 Cm Sm Finished Sequence
R471 78 kb IncL/M 50036 Ap Hg Finished Sequence
pIP135 68 kb IncL/M 50107 Gm Sm Su Tc Hg Finished Sequence
N3 50 kb IncN 50124 Sm Sp Su Tc Hg [HspII] In Finishing -
R753 102 kb IncV 50521 Ap Cm Sm Su Planned -
R7K 30 kb IncW 50006 Ap Sm Sp Published Sequence
pIP71 68 kb Com9 50106 Ap Cm Sm Sp Su Tc Finished Sequence
R6K 39 kb IncX 50005 Ap Sm Finished Sequence
R485 54 kb IncX 50040 Su Planned -
pHH1508a 208 kb IncH - Sm Sp Tp Tel Phi In Finishing -
MIP233 231 kb IncHI3 - Scr Phi Tel In Finishing -
pLV501 96.5 kb IncFII - Cm Km Tc Hg Finished Sequence
Legend

Ap - ampicillin; Su - sulphonamide; Gm - gentamycin; Cm - chloramphenicol; Tc - tetracycline; Tp - trimethoprim; Sm - streptomycin; Sp - spectinomycin; Km - kanamycin; Hg - mercury resistance; Tel - tellurite resistance; Phi - resistance to various bacteriophages; Scr - sucrose fermentation; [ ] square brackets - restriction-modification system

* quick link - http://q.sanger.ac.uk/efse503o