Pseudomonas

Pseudomonads are ubiquitous organisms distributed widely in the environment, including the soil and water and in association with various living host organisms. There are representatives such as Pseudomonas aeruginosa that represent one of the most prevalent causes of opportunistic infections in humans and is the most common cause of eventually fatal, persistent respiratory infections in cystic fibrosis (CF) patients. Others such as Pseudomonas fluorescens represent a physiologically diverse species that contributes greatly to the turnover of organic matter and, while present in soil, is abundant on the surfaces of plant roots and leaves. Of the plant-colonizing strains, some isolates are known to positively affect plant health and nutrition. The mechanistic bases of these effects remain unclear, but are known to include the production of plant-growth hormones, the suppression of pathogens (especially fungi and oomycetes) detrimental to plant health via competitive and / or allelopathic effects, and the direct elicitation of plant defence responses.

Data Downloads

[Genome Research Limited]

To date we have determined the genome sequence of the Liverpool Epidemic Strain (LES) of P. aeruginosa and Pseudomonas fluorescens strain SBW25

Main Collaborators

C. Winstanley Division of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Liverpool L69 3GA, United Kingdom

Stuart B. Levy Centre for Adaptation Genetics and Drug Resistance, Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA.

Paul B. Rainey New Zealand Institute for Advanced Study, Massey University, Private Bag 102 904, North Shore Mail Centre, Auckland, New Zealand.

Mark W. Silby Centre for Adaptation Genetics and Drug Resistance, Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA.

Published Genome Data

Pseudomonas aeruginosa

We have determined the genome sequence of Pseudomonas aeruginosa. The full annotation is available in the EMBL database with accession number FM209186. The genome is 6,601,757 bp in size with a G+C content of 66.3% and is available for searching on our Blast Server, or for download from our FTP site.

Shotgun and assembly data from these projects are available from our FTP site.


Pseudomonas fluorescens strain SBW25

We have determined the genome sequence of Pseudomonas fluorescens strain SBW2. The full annotation is available in the EMBL database with accession number AM181176. The genome is 6,722,540 bp in length, with an average G+C content of 60.5 %

We have also sequenced pQBR103, a plasmid from P. fluorescens. The full annotation is available in the EMBL database with accession number AM235768. The sequence is 425,094 bp with a G+C content of 53.15%.

Shotgun and assembly data from these projects are available from our FTP site.

Studies

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  • Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool Epidemic Strain of Pseudomonas aeruginosa.

    Winstanley C, Langille MG, Fothergill JL, Kukavica-Ibrulj I, Paradis-Bleau C, Sanschagrin F, Thomson NR, Winsor GL, Quail MA, Lennard N, Bignell A, Clarke L, Seeger K, Saunders D, Harris D, Parkhill J, Hancock RE, Brinkman FS and Levesque RC

    Genome research 2009;19;1;12-23

    PUBMED: 19047519; PMC: 2612960; DOI: 10.1101/gr.086082.108

  • Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens.

    Silby MW, Cerdeño-Tárraga AM, Vernikos GS, Giddens SR, Jackson RW, Preston GM, Zhang XX, Moon CD, Gehrig SM, Godfrey SA, Knight CG, Malone JG, Robinson Z, Spiers AJ, Harris S, Challis GL, Yaxley AM, Harris D, Seeger K, Murphy L, Rutter S, Squares R, Quail MA, Saunders E, Mavromatis K, Brettin TS, Bentley SD, Hothersall J, Stephens E, Thomas CM, Parkhill J, Levy SB, Rainey PB and Thomson NR

    Genome biology 2009;10;5;R51

    PUBMED: 19432983; PMC: 2718517; DOI: 10.1186/gb-2009-10-5-r51

  • Sequence-based analysis of pQBR103; a representative of a unique, transfer-proficient mega plasmid resident in the microbial community of sugar beet.

    Tett A, Spiers AJ, Crossman LC, Ager D, Ciric L, Dow JM, Fry JC, Harris D, Lilley A, Oliver A, Parkhill J, Quail MA, Rainey PB, Saunders NJ, Seeger K, Snyder LA, Squares R, Thomas CM, Turner SL, Zhang XX, Field D and Bailey MJ

    The ISME journal 2007;1;4;331-40

    PUBMED: 18043644; PMC: 2656933; DOI: 10.1038/ismej.2007.47

Related links

Data Use Statement

This sequencing centre plans on publishing the completed and annotated sequences in a peer-reviewed journal as soon as possible. Permission of the principal investigator should be obtained before publishing analyses of the sequence/open reading frames/genes on a chromosome or genome scale. See our data sharing policy.

Sequencing enquiries

Please address all sequencing enquiries to: pathinfo@sanger.ac.uk

* quick link - http://q.sanger.ac.uk/ff8x4ttp