Published Genome Data

The genome of Legionella pneumophila was sequenced using paired-end 454 FLX pyrosequencing and assembled using the 454/Roche Newbler assembly program into 274 contigs. These contigs were scaffolded using paired read into 11 scaffolds. Several contig gaps were closed using PCR and Sanger sequencing in conjunction with manual examination of the individual 454 reads in Consed. Unassembled contigs of less than 300bp were removed from the end of the assembly. This gave a total sequence length of 3,473,547 bp assembled into 145 contigs in 4 scaffolds plus 14 small contigs (114 – 3270 bp) which could not be scaffolded.

The genome has been sequenced in collaboration with Prof. Gad Frankel (Imperial College) and is available from the EMBL/GenBank databases with accession number FR687201.

• Legionella pneumophila strain 130b possesses a unique combination of type IV secretion systems and novel Dot/Icm secretion system effector proteins.

  Schroeder GN, Petty NK, Mousnier A, Harding CR, Vogrin AJ, Wee B, Fry NK, Harrison TG, Newton HJ, Thomson NR, Beatson SA, Dougan G, Hartland EL and Frankel G

  Journal of bacteriology 2010;192;22;6001-16

  PUBMED: 20833813; PMC: 2976443; DOI: 10.1128/JB.00778-10