The Sanger Institute was funded to perform comparative sequencing of five Escherichia coli and Shigella strains in collaboration with Dr. Christoph Tang of the Centre for Molecular Microbiology and Infection, Imperial College, London, Prof. Mark Pallen and Dr. Ian Henderson of the Division of Immunity and Infection, University of Birmingham, UK Dr. Claude Parsot and Dr. Phillipe Sansonetti of the Unite de Pathologie Microbienne, Institut Pasteur, France, Dr. Gadi Frankel, of the Department of Biochemistry, Imperial College, London, and Dr. Stuart Knutton of the Institute of Child Health, Birmingham.
Published Genome Data
Published Sequence
E. coli E2348/69 is published and consists of a chromosome, with EMBL/GenBank accession FM180568, and two plasmids, with EMBL/GenBank accessions FM180569 and FM180570.
Shotgun and assembly data from this project are available from our FTP site.
E. coli 042 is published and consists of a chromosomal genome, with EMBL/GenBank accession FN554766 and a plasmid, with EMBL/GenBank accession FN554767.
Shotgun and assembly data from this project are available from our FTP site.
The chromosomal genomes ofE.coli chi7122 and E. coli IMT2125 are available in EMBL/GenBank with accessions HE962388 and HE964769 respectively.
Funded Sequencing
E. coli non-K1 which has been funded for sequencing.
Other Projects
Additionally, as part of a joint project investigating the genomics and population biology of Enterotoxigenic E. coli (ETEC), the Sanger Institute, funded by the BBSRC has sequenced the genome of an ETEC strain, H10407 This project was funded in collaboration with Dr. Ian Henderson and Prof. Mark Pallen of the Department of Immunity and Infection, University of Birmingham.
This genome is published and consists of a chromosome and 4 plasmids. The chromosome has EMBL/GenBank accession FN649414 and the plasmids have accessions FN649415, FN649416, FN649417 and FN649418
Shotgun and assembly data from this project are available from our FTP site.
Studies
- Haemophilus parasuis genome comparisons
- Global phylogeny of enterotoxigenic Escherichia coli collection
- CF negative Enterotoxigenic Escherichia coli
- Atypical enteropathogenic E. coli virulence markers
- Global diversity of Shigella species
- Relationship of MLST structure of Escherichia coli species to genomic organisation complexity
- Defining EPEC: evolution, global distribution and effector inventory
- Comparative genome sequence analysis of the globally disseminated Escherichia coli ST131 lineage
- Whole genome sequencing of naturally isolated Escherichia coli from nursing homes in Ireland
- Clostridium difficile 078 diversity
- Antimicrobial resistance modifiers studied by TraDIS
- HPACC Murray project
- Retrospective 0157:H7 TraDIS from screen in calves
- ETEC wild types and attenuated vaccine strains
Haemophilus parasuis genome comparisons
Sample | Strain | Run Accession |
|---|---|---|
| 121113_ec | Unknown | ERR225615 |
Global phylogeny of enterotoxigenic Escherichia coli collection
CF negative Enterotoxigenic Escherichia coli
Atypical enteropathogenic E. coli virulence markers
Global diversity of Shigella species
Sample | Strain | Run Accession |
|---|---|---|
| sh1290 | K-3051 | ERR212270 |
| sh1289 | 1069 C1 | ERR212269 |
| sh1272 | 3101 | ERR217072 |
| sh1271 | 3080 | ERR217071 |
| sh1270 | 2975 | ERR217070 |
| sh1269 | 2834 | ERR217069 |
| sh1268 | 2795 | ERR217068 |
| sh1267 | 3204 | ERR217067 |
| sh1266 | 3143 | ERR217066 |
| sh1265 | 3138 | ERR217065 |
| sh1264 | 3073 | ERR217064 |
| sh1263 | 3072 | ERR217063 |
| sh1262 | 2808 | ERR217062 |
| sh1261 | 2647 | ERR217061 |
Relationship of MLST structure of Escherichia coli species to genomic organisation complexity
Defining EPEC: evolution, global distribution and effector inventory
Comparative genome sequence analysis of the globally disseminated Escherichia coli ST131 lineage
Whole genome sequencing of naturally isolated Escherichia coli from nursing homes in Ireland
Clostridium difficile 078 diversity
Sample | Strain | Run Accession |
|---|---|---|
| Birm7 | Unknown | ERR171252 |
| Birm6 | Unknown | ERR171251 |
| Birm5 | Unknown | ERR171250 |
| Birm4 | Unknown | ERR171249 |
| Birm3 | Unknown | ERR171248 |
| Birm2 | Unknown | ERR171247 |
| Birm1 | Unknown | ERR171246 |
Antimicrobial resistance modifiers studied by TraDIS
HPACC Murray project
Sample | Strain | Run Accession |
|---|---|---|
| NCTC-M291 | M291 | ERR204290 |
| NCTC-M20 | M20 | ERR204289 |
| 2359STDY5403533 | M128 | ERR230465 |
| 2359STDY5403525 | M127 | ERR230457 |
| 2359STDY5403519 | M177 | ERR230451 |
| 2359STDY5403518 | M143 | ERR230450 |
| 2359STDY5403517 | M126 | ERR230449 |
| 2359STDY5403504 | M228 | ERR230436 |
| 2359STDY5403495 | M163 | ERR230427 |
| 2359STDY5403465 | M256 | ERR230397 |
| 2359STDY5403443 | M41 | ERR230375 |
Retrospective 0157:H7 TraDIS from screen in calves
Sample | Strain | Run Accession |
|---|---|---|
| O157_Input | 0157:H7 | ERR022652 |
| O157_Output | 0157:H7 | ERR022653 |
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A commensal gone bad: complete genome sequence of the prototypical enterotoxigenic Escherichia coli strain H10407.
Journal of bacteriology 2010;192;21;5822-31
PUBMED: 20802035; PMC: 2953697; DOI: 10.1128/JB.00710-10
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Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042.
PloS one 2010;5;1;e8801
PUBMED: 20098708; PMC: 2808357; DOI: 10.1371/journal.pone.0008801
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Complete genome sequence and comparative genome analysis of enteropathogenic Escherichia coli O127:H6 strain E2348/69.
Journal of bacteriology 2009;191;1;347-54
PUBMED: 18952797; PMC: 2612414; DOI: 10.1128/JB.01238-08

