Clostridium

Bacteria of the genus Clostridium are Gram positive, obligate anaerobes capable of producing endospores. Species include:

  • Clostridium botulinum which produces botulinum neurotoxin, which is the cause of foodborne botulism.
  • Clostridium sordellii, a gram-positive, spore forming anaerobic bacterium associated with rare but severe infections of both humans and animals.

Please Note: Due to recent changes in phylogeny and taxonomy, data from Clostridium difficile formerly found on this page can now be found on the Peptoclostridium difficile page.

Clostridium botulinum

The Sanger Institute has completed the sequence of the genome of Clostridium botulinum, in collaboration with Dr. Roger Huston of the Food Microbial Sciences Unit, University of Reading Department of Food Science, Dr. Nigel Minton of the Institute of Infection, Immunity and Inflammation, University of Nottingham, and Dr. M. Peck of the Food Saftey Microbiology Section, Institute of Food Research.

We have sequenced Hall strain A (ATCC 3502) and Eklund 17B. The genome of Hall strain A (ATCC 3502) is 3,886,916 bp in size, with a G+C content of approximately 28.2%. There is also a plasmid of 16,344 bp. The completed sequence for both strains is available for searching on our Blast server, or for download from our FTP site. This project is funded by Beowulf Genomics.

The fully annotated genome and plasmid are available of Hall strain A (ATCC 3502) are available from the EMBL/GenBank databases with accession numbers AM412317 and AM412318 respectively.

Shotgun and assembly data from this project are available from our FTP site.

Clostridium sordellii

We have completed the sequencing, assembly and annotation of 44 diverse strains of Clostridium sordellii. All are isolated from incidences of clinical or veterinary disease and are from diverse geographical sources- see table below. This work was carried out in collaboration with Professor Neil Fairweather of Imperial College London. An Optical Map was generated for strain ATCC9714 here at the WTSI to improve and validate the initial genome assembly in addition to isolating plasmid sequence.

For full details of all sequenced strains and links to the annotated assemblies please see the C. sordellii page.

Studies

MetaHIT Genome Improvement
SampleStrainRun Accession
C_saccharolyticum_like_K10K10ERR037811
250510-53UnknownERR043353
SampleStrainRun Accession
2305_30230511_30ERR056965
1805pm_95180511pm_95ERR056982
16JANC8180511pm_93ERR152469
2709am_56230511_30ERR152454
2709am_1230511_25ERR152453
Lung Culturomics
SampleStrainRun Accession
9_Run_RUnknownERR968252

Bibliography

  • Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes.

    Sebaihia M, Peck MW, Minton NP, Thomson NR, Holden MT, Mitchell WJ, Carter AT, Bentley SD, Mason DR, Crossman L, Paul CJ, Ivens A, Wells-Bennik MH, Davis IJ, Cerdeño-Tárraga AM, Churcher C, Quail MA, Chillingworth T, Feltwell T, Fraser A, Goodhead I, Hance Z, Jagels K, Larke N, Maddison M, Moule S, Mungall K, Norbertczak H, Rabbinowitsch E, Sanders M, Simmonds M, White B, Whithead S and Parkhill J

    Genome research 2007;17;7;1082-92

Data Use Statement

This sequencing centre plans on publishing the completed and annotated sequences in a peer-reviewed journal as soon as possible. Permission of the principal investigator should be obtained before publishing analyses of the sequence/open reading frames/genes on a chromosome or genome scale. See our data sharing policy.

Sequencing enquiries

Please address all sequencing enquiries to: pathinfo@sanger.ac.uk

Short-link