Burkholderia pseudomallei

Burkholderia pseudomallei (previously called Pseudomonas pseudomallei) is the causative agent of melioidosis, a serious disease of man and animals that occurs primarily in S.E. Asia, N. Australia and other tropical areas. B. pseudomallei is an environmental Gram-negative saprophyte present in wet soil and rice paddies in endemic areas. The highest documented infection rate is in northeastern Thailand, where melioidosis accounts for 20% of all community-acquired septicaemias. Disease occurs after bacterial contamination of breaks in the skin or by inhalation after contact with water or soil. There is no licensed vaccine against meliodosis, and the bacterium is resistant to many antibiotics. B. pseudomallei strain K96243 is a clinical isolate from Thailand, supplied by Dr. S. Songsivilai of Mahidol University.

Data Downloads

BLAST server

FTP site

[Genome Research Limited]

The Sanger Institute has published the 7.2 Mb genome of Burkholderia pseudomallei in collaboration with Dr. Rick Titball of the Defence Science and Technology Laboratory, Porton Down, and Dr. Ty Pitt of the Heath Protection Agency. We have sequenced strain K96243, a clinical isolate from Thailand, supplied by Dr. S. Songsivilai of Mahidol University. This project is funded by Beowulf Genomics.

Published Genome Data

The reference genome is 7,247,547 bp in size with a G+C content of 68.06% consisting of two chromosomes of 4,074,542 bp and 3,173,005 bp. The sequence is available for download by FTP and can be searched using our BLAST server.

The two fully annotated chromosomal sequences are deposited in the EMBL/GenBank databases with accession numbers BX571965 and BX571966.

Shotgun and assembly data from this project are available from the FTP link on the right.

Discovery of sequence diversity in Burkholderia pseudomallei infection

Sample

Strain

Run Accession

1x13921ERR027434
1x23921.0ERR027437
1x33921.0ERR027436
1x43921.0ERR027438
1x53921.0ERR027439
1x63921.0ERR027440
1x73921.0ERR027441
1x83921.0ERR027442
1x93921.0ERR027443
1x103921.0ERR027435
2x13921.0ERR023997
2x23921.0ERR023999
2x33921.0ERR024000
2x43921.0ERR024001
2x53921.0ERR024002
2x63921.0ERR024003
2x73921.0ERR024004
2x83921.0ERR024005
2x93921.0ERR024006
2x103921.0ERR023998
3x13921.0ERR024008
3x23921.0ERR024010
3x33921.0ERR024011
3x43921.0ERR024012
3x53921.0ERR024013
3x63921.0ERR024014
3x73921.0ERR024015
3x83921.0ERR024016
3x93921.0ERR024017
3x103921.0ERR024009
4x13921.0ERR024019
4x23921.0ERR024021
4x33921.0ERR024022
4x43921.0ERR024023
4x43921.0ERR152370
4x53921.0ERR024024
4x53921.0ERR152371
4x63921.0ERR024025
4x63921.0ERR152372
4x73921.0ERR024026
4x73921.0ERR152373
4x83921.0ERR024027
4x83921.0ERR152374
4x93921.0ERR024028
4x93921.0ERR152375
4x103921.0ERR024020
4x103921.0ERR152376
5x13921.0ERR024030
5x13921.0ERR152377
5x23921.0ERR024032
5x23921.0ERR152378
5x33921.0ERR024033
5x33921.0ERR152379
5x43921.0ERR024034
5x43921.0ERR152380
5x53921.0ERR024035
5x53921.0ERR152381
5x63921.0ERR024036
5x63921.0ERR152382
5x73921.0ERR024037
5x73921.0ERR152383
5x83921.0ERR024038
5x83921.0ERR152384
5x93921.0ERR024039
5x93921.0ERR152385
5x103921.0ERR024031
5x103921.0ERR152386
6x13921.0ERR024041
6x13921.0ERR152387
6x23921.0ERR024043
6x23921.0ERR152388
6x33921.0ERR024044
6x33921.0ERR152389
6x43921.0ERR024045
6x43921.0ERR152390
6x53921.0ERR024046
6x53921.0ERR152391
6x63921.0ERR024047
6x63921.0ERR152392
6x73921.0ERR024048
6x73921.0ERR152393
6x83921.0ERR024049
6x83921.0ERR152394
6x93921.0ERR024050
6x93921.0ERR152395
6x103921.0ERR024042
6x103921.0ERR152396
7x13921.0ERR024052
7x13921.0ERR152397
7x23921.0ERR024054
7x23921.0ERR152398
7x33921.0ERR024055
7x33921.0ERR152399
7x43921.0ERR024056
7x43921.0ERR152400
7x53921.0ERR024057
7x53921.0ERR152401
7x63921.0ERR024058
7x63921.0ERR152402
7x73921.0ERR024059
7x73921.0ERR160883
7x83921.0ERR024060
7x83921.0ERR160884
7x93921.0ERR024061
7x93921.0ERR160885
7x103921.0ERR024053
7x103921.0ERR160886

Burkholderia pseudomallei diversity

Sample

Strain

Run Accession

619s020BP48ERR026263
619s020BP48ERR035382
619s021BP54ERR026267
619s021BP54ERR035386
619s022BP56ERR026268
619s022BP56ERR035387
619s023BP59ERR026269
619s023BP59ERR035388
619s024KASERR026270
619s024KASERR035389
619s025JAMERR035390
619s025JAMERR026271
619s026BP107ERR026272
619s026BP107ERR035391
619s027BP115ERR026273
619s027BP115ERR035392
619s0284090-0390ERR026274
619s0284090-0390ERR035393
619s029EM1157ERR035383
619s029EM1157ERR026264
619s030EB5661ERR026265
619s030EB5661ERR035384
619s031AH4ERR026266
619s031AH4ERR035385
619s032AH3ERR026276
619s032AH3ERR035395
619s0334094-0081ERR026280
619s0334094-0081ERR035399
619s034561.0ERR035400
619s034561.0ERR026281
619s0354094-225ERR026282
619s0354094-225ERR035401
619s036G-ShephardERR026283
619s036G-ShephardERR035402
619s037612.0ERR035403
619s037612.0ERR026284
619s038DB19897/02ERR026285
619s038DB19897/02ERR035404
619s03927/96ERR026286
619s03927/96ERR035405
619s040153.0ERR026287
619s040153.0ERR035406
619s040153.0ERR156300
619s0414009-19ERR026277
619s0414009-19ERR035396
619s0414009-19ERR156301
619s042DR13450/01ERR026278
619s042DR13450/01ERR035397
619s042DR13450/01ERR156302
619s043EM2107ERR026279
619s043EM2107ERR035398
619s043EM2107ERR156303
619s04415/96ERR026289
619s04415/96ERR035408
619s04415/96ERR156304
619s0454033-10ERR026293
619s0454033-10ERR035412
619s0454033-10ERR156305
619s046DB30729/00ERR035413
619s046DB30729/00ERR026294
619s046DB30729/00ERR156306
619s047DB61091/00ERR026295
619s047DB61091/00ERR035414
619s047DB61091/00ERR156307
619s048DR08726/01ERR026296
619s048DR08726/01ERR035415
619s048DR08726/01ERR156308
619s049EM10266/01ERR026297
619s049EM10266/01ERR035416
619s049EM10266/01ERR156309
619s050488.0ERR026298
619s050488.0ERR035417
619s050488.0ERR156310
619s05121/96ERR026299
619s05121/96ERR035418
619s05121/96ERR156311
619s052497/96ERR026300
619s052497/96ERR035419
619s052497/96ERR156312
619s05310/96ERR026290
619s05310/96ERR035409
619s05310/96ERR156313
619s054457/96ERR035410
619s054457/96ERR026291
619s054457/96ERR156314
619s055504/96ERR026292
619s055504/96ERR035411
619s055504/96ERR156315
619s05617/96ERR026302
619s05617/96ERR035421
619s05617/96ERR156316
619s0576/96ERR026306
619s0576/96ERR035425
619s0576/96ERR156317
619s05835/96ERR026307
619s05835/96ERR035426
619s05835/96ERR156318
619s05923/96ERR035427
619s05923/96ERR026308
619s05923/96ERR156319
619s060D107310-3154ERR026309
619s060D107310-3154ERR035428
619s060D107310-3154ERR156320
619s061K11277244-293ERR035429
619s061K11277244-293ERR026310
619s061K11277244-293ERR156321
619s062I64043-3096ERR026311
619s062I64043-3096ERR035430
619s062I64043-3096ERR156322
619s063P73230-3018ERR026312
619s063P73230-3018ERR035431
619s063P73230-3018ERR156323
619s064DBERR026313
619s064DBERR035432
619s064DBERR156324
619s06577/96ERR026303
619s06577/96ERR035422
619s06577/96ERR156325
619s066DCERR026304
619s066DCERR035423
619s066DCERR156326
619s067109/96ERR026305
619s067109/96ERR035424
619s067109/96ERR156327
619s068490.0ERR026315
619s068490.0ERR035434
619s068490.0ERR156328
619s069SOILERR026318
619s069SOILERR035437
619s069SOILERR156329
619s070D68346-3058ERR026319
619s070D68346-3058ERR035438
619s070D68346-3058ERR156330
619s07178/96ERR026320
619s07178/96ERR035439
619s07178/96ERR156331
619s07279/96ERR026321
619s07279/96ERR035440
619s07279/96ERR156332
619s073P163/04ERR026322
619s073P163/04ERR035441
619s073P163/04ERR160916
619s074EY10ERR026323
619s074EY10ERR035442
619s074EY10ERR160917
619s075P165/04ERR035443
619s075P165/04ERR026324
619s075P165/04ERR160918
619s076169/04ERR026325
619s076169/04ERR035444
619s076169/04ERR160919
619s077170/04ERR026316
619s077170/04ERR035435
619s077170/04ERR160920
619s078SW1ERR026317
619s078SW1ERR035436
619s078SW1ERR160921
619s07912-40ERR026327
619s07912-40ERR035446
619s07912-40ERR160922
619s080164/04ERR026330
619s080164/04ERR035449
619s080164/04ERR160923
619s081EY9ERR026331
619s081EY9ERR035450
619s081EY9ERR160924
619s082SW9ERR035451
619s082SW9ERR026332
619s082SW9ERR160925
619s083P166/04ERR026333
619s083P166/04ERR035452
619s083P166/04ERR160926
619s08415-10ERR026334
619s08415-10ERR035453
619s08415-10ERR160927
619s085TRF661ERR026335
619s085TRF661ERR035454
619s085TRF661ERR160928
619s086P171/04ERR026336
619s086P171/04ERR035455
619s086P171/04ERR160929
619s087P157/06ERR026337
619s087P157/06ERR035456
619s087P157/06ERR160930
619s08815-40ERR026328
619s08815-40ERR035447
619s08815-40ERR160931
619s089413.0ERR026329
619s089413.0ERR035448
619s089413.0ERR160932
619s090EB6103/04ERR026728
619s090EB6103/04ERR035458
619s090EB6103/04ERR160933
619s0914086-21ERR026731
619s0914086-21ERR035461
619s0914086-21ERR160934
619s09235.0ERR035462
619s09235.0ERR026732
619s09235.0ERR160935
619s093E387ERR026733
619s093E387ERR035463
619s093E387ERR160936
619s094AM1ERR026734
619s094AM1ERR035464
619s094AM1ERR160937
619s0954044-138ERR026735
619s0954044-138ERR035465
619s0954044-138ERR160938
619s096147.0ERR026736
619s096147.0ERR035466
619s096147.0ERR160939
619s097DM15342/00ERR026737
619s097DM15342/00ERR035467
619s097DM15342/00ERR160940
619s098 2746BERR026738
619s098 2746BERR035468
619s098 2746BERR160941
619s0992-96ERR026729
619s0992-96ERR035459
619s0992-96ERR160942
619s100DI9033-508ERR026730
619s100DI9033-508ERR035460
619s100DI9033-508ERR160943
619s001ATCC15682ERR028479
619s001ATCC15682ERR033174
619s001ATCC15682ERR069576
619s002P6ERR028483
619s002P6ERR033178
619s002P6ERR069577
619s003ATCC23343ERR028484
619s003ATCC23343ERR033179
619s003ATCC23343ERR069578
619s004EY1ERR028485
619s004EY1ERR033180
619s004EY1ERR069579
619s005EY2ERR028486
619s005EY2ERR033181
619s005EY2ERR069580
619s006EY3ERR028487
619s006EY3ERR033182
619s006EY3ERR069581
619s007EY4ERR028488
619s007EY4ERR033183
619s007EY4ERR069582
619s008EY5ERR028489
619s008EY5ERR033184
619s008EY5ERR069583
619s009EY6ERR028490
619s009EY6ERR033185
619s009EY6ERR069584
619s010EY7ERR028480
619s010EY7ERR033175
619s010EY7ERR069585
619s011EY8ERR028481
619s011EY8ERR033176
619s012BP4ERR028482
619s012BP4ERR033177
619s013BP6ERR033161
619s013BP6ERR026250
619s014BP8ERR026254
619s014BP8ERR033165
619s015BP10ERR026255
619s015BP10ERR033166
619s016BP11ERR033167
619s016BP11ERR026256
619s017BP17ERR026257
619s017BP17ERR033168
619s018BP20ERR033169
619s018BP20ERR026258
619s019BP33ERR033170
619s019BP33ERR026259
619s101Bps708aERR033171
619s101Bps708aERR026260
619s101Bps708aERR160944
619s102BpsHAERR033172
619s102BpsHAERR026261
619s102BpsHAERR160945
619s103BpsBRIERR026251
619s103BpsBRIERR033162
619s103BpsBRIERR160946
619s104BpsE8ERR026252
619s104BpsE8ERR033163
619s104BpsE8ERR160947
619s105BpsE25ERR026253
619s105BpsE25ERR033164
619s105BpsE25ERR160948
3921gUnknownERR069586
  • Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei.

    Holden MT, Titball RW, Peacock SJ, Cerdeño-Tárraga AM, Atkins T, Crossman LC, Pitt T, Churcher C, Mungall K, Bentley SD, Sebaihia M, Thomson NR, Bason N, Beacham IR, Brooks K, Brown KA, Brown NF, Challis GL, Cherevach I, Chillingworth T, Cronin A, Crossett B, Davis P, DeShazer D, Feltwell T, Fraser A, Hance Z, Hauser H, Holroyd S, Jagels K, Keith KE, Maddison M, Moule S, Price C, Quail MA, Rabbinowitsch E, Rutherford K, Sanders M, Simmonds M, Songsivilai S, Stevens K, Tumapa S, Vesaratchavest M, Whitehead S, Yeats C, Barrell BG, Oyston PC and Parkhill J

    Proceedings of the National Academy of Sciences of the United States of America 2004;101;39;14240-5

Data Use Statement

This sequencing centre plans on publishing the completed and annotated sequences in a peer-reviewed journal as soon as possible. Permission of the principal investigator should be obtained before publishing analyses of the sequence/open reading frames/genes on a chromosome or genome scale. See our data sharing policy.

Sequencing enquiries

Please address all sequencing enquiries to: pathinfo@sanger.ac.uk

* quick link - http://q.sanger.ac.uk/q000hcue