Bordetella

The genus Bordetella comprises Gram-negative beta-proteobacteria including three species which are human pathogens; Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica. B. pertussis, and occasionally B. parapertussis, can cause whooping cough in human infants. B. pertussis only infects humans while B. parapertussis infects both humans and sheep. Phylogenetic analyses have shown that B. parapertussis strains isolated from humans are distinct from those isolated from sheep, and there is little or no transmission between the two reservoirs (sheep and human). B. bronchiseptica colonize the respiratory tracts of a range of mammals, but can also persist in the environment for extended periods. It causes chronic and often asymptomatic respiratory infections in a wide range of animals, but only occasionally in humans. A related species, B. avium causes disease (bordetellosis) in commercially grown turkeys, but is also found in a variety of healthy wild and domesticated birds.

Data downloads

[Genome Research Limited]

The Sanger Institute has been funded by the Wellcome Trust to sequence the genomes of Bordetella pertussis strain Tohama I, B. parapertussis strain 12822 and B. bronchiseptica strain RB50 in collaboration with Duncan Maskell and Andrew Preston of the Centre for Veterinary Science, Dept. of Clinical Veterinary medicine, The University of Cambridge. The sequences and analysis are described in: Parkhill et al (2003) Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica. Nature Genetics 35 32-40 (DOI: 10.1038/Ng1227), and have been submitted to EMBL/GenBank with the accession numbers: BX470248 (B. pertussis), BX470249 (B. parapertussis) and BX470250 (B. bronchiseptica).

The three sequenced Bordetella strains have been deposited with the ATCC and NCTC under the following accession numbers: Bordetella parapertussis 12822: ATCC BAA-587, NCTC 13253 Bordetella bronchiseptica RB50: ATCC BAA-588, NCTC 13252 Bordetella pertussis Tohama I: ATCC BAA-589, NCTC 13251

We have have also sequenced three closely related isolates of a B. pertussis bacteriophage that exhibited a novel mechanism of host range variation. The mechanism is described in Liu et al, Science 295, 2091 (2002), and the bacteriophage sequences were published in Liu et al, J Bacteriol 186, 1503-1517 (2004), and are available from Genbank/EMBL as: AY029185, AY526908 and AY526909.

We have also been funded by the USDA to sequence the genome of Bordetella avium strain 197N in collaboration with Louise Temple of the Department of Biology, James Madison University

Bordetella bronchiseptica complex sequencing

The Sanger Institute has completed a project to sequence further strains within the Bordetella bronchiseptica complex to investigate the evolutionary history and host tropism differences within this group of organsims. The strain selection is based on the MLST tree published in Diavatopoulos et al, PLoS Pathog. 1, e45 (2005).

This work is being done in collaboration with Dr. Craig Cummings and Prof. David Relman (Relman Lab, Dept. of Microbiology and Immunology, SUMC, Stanford University), Prof. Eric Harvill (Centre for Infectious Disease Dynamics, Penn State University), Dr. Andrew Preston (Univeristy of Bristol), Dr. Frits Mooi (Laboratory for Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, Bilthoven, Netherlands) and Dr. Jeff F. Miller (Department of Micribiology, Immunology and Molecular Genetics, UCLA).

The strains selected for sequencing included :

B. bronchiseptica strain 253 (ST27)

B. pertussis strain 18323 (ST24)

B. parapertussis (ovine) Bpp5 (ST16)

B. bronchiseptica strain MO149 (ST15)

B. bronchiseptica strain D445

B. bronchiseptica Bbr77

B. bronchiseptica strain 1289

This project is funded by the Wellcome Trust.

Published Genome Data

The fully sequenced and annotated reference genomes are available from EMBL/GenBank as follows :-

BX470248 (B. pertussis Tohama I)

BX470249 (B. parapertussis 12822)

BX470250 (B. bronchiseptica RB50).

AM167904 (B. avium strain 197N)

HE965805 (B. pertussis strain 18323 (ST24))

HE983626 (B. bronchiseptica strain 1289)

HE965806 (B. bronchiseptica strain 253 (ST27))

HE983628 (B. bronchiseptica strain Bbr77)

HE983627 (B. bronchiseptica strain D445)

HE965807 (B. bronchiseptica strain MO149 (ST15))

HE965803 (B. parapertussis strain Bpp5 (ST16) chromosome)

HE965804 (B. parapertussis strain Bpp5 (ST16) plasmid)

Shotgun and assembly data from these projects are available from our FTP site.

Bordetella diversity project

Sample

Strain

Run Accession

L452L452ERR023405
L453L453ERR023409
L535L535ERR023410
L570L570ERR023411
L454L454ERR023412
L467L467ERR023413
L468L468ERR023414
L481L481ERR023415
L491L491ERR023416
L504L504ERR023406
L508L508ERR023407
L518L518ERR023408
10701070ERR361869
10801080ERR030033
10801080ERR361870
PT3764PT3764ERR030034
PT3764PT3764ERR361871
PT3778PT3778ERR030035
PT3778PT3778ERR361872
1153011530ERR030036
1153011530ERR361873
PT1487PT1487ERR030037
PT1487PT1487ERR361874
PT1659PT1659ERR361875
PT1659PT1659ERR030038
PT1877PT1877ERR030039
PT1877PT1877ERR361876
PT1879PT1879ERR030040
PT1879PT1879ERR361877
PT2019PT2019ERR030030
PT2019PT2019ERR361878
PT2400PT2400ERR030031
PT2400PT2400ERR361879
PT2689PT2689ERR361880
PT2689PT2689ERR030032
B0402_2B0402ERR361821
B0497_2B0497ERR361831
B3175_2B3175ERR361830
B2973_2B2973ERR361829
B0592_2B0592ERR361828
B1223_2B1223ERR361827
B1197_2B1197ERR361826
B1195_2B1195ERR361825
B0904_2B0904ERR361824
B1836_2B1836ERR361823
B3190_2B3190ERR361822
B3189_2B3189ERR361832
B2030_2B2030ERR361833
B3197_2B3197ERR361843
F156_2F156ERR361842
F039_2F039ERR361841
B3200_2B3200ERR361840
B3199_2B3199ERR361839
A639_2A639ERR361838
A020_2A020ERR361837
E189_2E189ERR361836
F366_2F366ERR361835
F243_2F243ERR361834
F208_2F208ERR361844
L702_2L702ERR361845
L682_2L682ERR361855
L601_2L601ERR361854
E503_2E503ERR361853
L681_2L681ERR361852
L676_2L676ERR361851
L675_2L675ERR361850
L673_2L673ERR361849
L672_2L672ERR361848
L559_2L559ERR361847
45680_245680ERR361846
A63_2A63ERR361856
B1374_2B1374ERR361857
B1878_2B1878ERR361867
RU140_2RU140ERR361866
RU171_2RU171ERR361865
B3188_2B3188ERR361864
B3178_2B3178ERR361863
B3195_2B3195ERR361862
B3193_2B3193ERR361861
B1900_2B1900ERR361860
B3191_2B3191ERR361859
B3202_2B3202ERR361858
B3198_2B3198ERR361868
IS5840_2IS5840ERR019587
IS8205_2IS8205ERR019589
2035970_22035970ERR019588
2146970_22146970ERR019598
1326620_21326620ERR019597
939980_2939980ERR019594
126690_2126690ERR019593
606770_2606770ERR019592
1758970_21758970ERR019590
3690970_23690970ERR019601
273980_2273980ERR019602
PT2019_2PT2019ERR019585
B0935_rptB0935ERR029291
B0908_rptB0908ERR029295
B0934_rptB0934ERR029296
B1173_rptB1173ERR029297
B0922_rptB0922ERR029298
B0907_rptB0907ERR029299
B1178_rptB1178ERR029300
B0930_rptB0930ERR029301
B1141_rptB1141ERR029302
B1134_rptB1134ERR029292
B1153_rptB1153ERR029293
B0919_rptB0919ERR029294
B1161_rptB1161ERR029304
B0917_rptB0917ERR029308
B1137_rptB1137ERR029309
B0913_rptB0913ERR029310
BP70_rptBP70ERR029311
BP22_rptBP22ERR029312
BP182_rptBP182ERR029313
BP120_rptBP120ERR029314
BP128_rptBP128ERR029315
BP121_rptBP121ERR029305
BP31_rptBP31ERR029306
BP56_rptBP56ERR029307
BP119_rptBP119ERR029317
BP143_rptBP143ERR029320
BP227_rptBP227ERR029321
BP230_rptBP230ERR029322
BP279_rptBP279ERR029323
BP310_rptBP310ERR029324
BP317_rptBP317ERR029325
BP6_rptBP6ERR029326
BP2_rptBP2ERR029327
BP7_rptBP7ERR029318
BP101_rptBP101ERR029319
PRCB637_rptPRCB637ERR029329
PRCB649_rptPRCB649ERR029333
PRCB521_rptPRCB521ERR029334
PRCB450_rptPRCB450ERR029335
PRCB640_rptPRCB640ERR029336
DK6_rptBp1504ERR029337
DK7_rptBp542ERR029338
DK8_rptBp2700ERR029339
DK10_rptBpH15814ERR029340
DK11_rptBpH29061ERR029330
DK12_rptBpT24287ERR029331
DK13_rptBpM29255ERR029332
DK14_rptBpH21652ERR029981
DK15_rptBpH31243ERR029985
Bp02-001_rptBp02-001ERR029986
Bp02-002_rptBp02-002ERR029987
Bp02-005_rptBp02-005ERR029988
Bp02-012_rptBp02-012ERR029989
Bp02-016_rptBp02-016ERR029990
Bp02-019_rptBp02-019ERR029991
Bp02-027_rptBp02-027ERR029992
Bp02-035_rptBp02-035ERR029982
Bp02-044_rptBp02-044ERR029983
Bp02-045_rptBp02-045ERR029984
bp5640_rptbp5640ERR028821
bp5822_rptbp5822ERR028825
bp3092_rptbp3092ERR028826
bp890_rptbp890ERR028827
bp4834_rptbp4834ERR028828
bp1773_rptbp1773ERR028829
bp5766_rptbp5766ERR028830
bp6334_rptbp6334ERR028831
bp5783_rptbp5783ERR028832
bp5776_rptbp5776ERR028822
bp1535_rptbp1535ERR028823
bp6632_rptbp6632ERR028824
Cz_rptCzERR028834
Hav_rptHavERR028837
FR145_rptFR145ERR028838
FR287_rptFR287ERR028839
FR743_rptFR743ERR028840
FR1003_rptFR1003ERR028841
FR1139_rptFR1139ERR028842
FR3080_rptFR3080ERR028843
FR3207_rptFR3207ERR028844
FR3407_rptFR3407ERR028835
FR3713_rptFR3713ERR028836
L452_rptL452ERR028846
L453_rptL453ERR028850
L454_rptL454ERR028851
L467_rptL467ERR028852
L468_rptL468ERR028853
L481_rptL481ERR028854
L491_rptL491ERR028855
L504_rptL504ERR028856
L508_rptL508ERR028857
L518_rptL518ERR028847
L535_rptL535ERR028848
L570_rptL570ERR028849
L573_rptL573ERR028859
L580_rptL580ERR028863
L581_rptL581ERR028864
L582_rptL582ERR028865
L584_rptL584ERR028866
L590_rptL590ERR028867
L595_rptL595ERR028868
L599_rptL599ERR028869
L604_rptL604ERR028870
L609_rptL609ERR028860
L610_rptL610ERR028861
L614_rptL614ERR028862
L617_rptL617ERR028872
L619_rptL619ERR028876
L638_rptL638ERR028877
L644_rptL644ERR028878
L646_rptL646ERR028879
L649_rptL649ERR028880
L655_rptL655ERR028881
L687_rptL687ERR028882
L695_rptL695ERR028883
L708_rptL708ERR028873
L709_rptL709ERR028874
L711_rptL711ERR028875
PT3783_rptPT3783ERR028885
BP92001_rptBP92001ERR028888
BP93001_rptBP93001ERR028889
BP94016_rptBP94016ERR028890
BP94025_rptBP94025ERR028891
BP94035_rptBP94035ERR028892
BP95003_rptBP95003ERR028893
BP95004_rptBP95004ERR028894
BP96004_rptBP96004ERR028895
BP96006_rptBP96006ERR028886
BP96013_rptBP96013ERR028887
BP105_rptBP105ERR031081
BP106_rptBP106ERR031082
BP312_rptBP312ERR031083
BP601_rptBP601ERR031084
BP706_rptBP706ERR031085
BP824_rptBP824ERR031086
BP6901_rptBP6901ERR031087
KKK22_rptKKK22ERR031088
KKK1277_rptKKK1277ERR031089
19773_rpt1977-3ERR029994
PRCB7H_rptPRCB7-HERR029995
PRCB2_rptPRCB2ERR029996
PRCB118_rptPRCB118ERR029997
PRCB179_rptPRCB179ERR029998
PRCB272_rptPRCB272ERR029999
PRCB352_rptPRCB352ERR030000
PRCB406_rptPRCB406ERR030001
KKK133_rptKKK1330ERR030002
A818_rptA818ERR030017
B159_rptB159ERR030021
B200_rptB200ERR030022
B202_rptB202ERR030023
B203_rptB203ERR030024
B215_rptB215ERR030025
B225_rptB225ERR030026
B316_rptB316ERR030027
B385_rptB385ERR030028
B453_rptB453ERR030018
B490_rptB490ERR030019
B733_rptB733ERR030020
C139_rptC139ERR030004
C455_rptC455ERR030008
C467_rptC467ERR030009
C477_rptC477ERR030010
C670_rptC670ERR030011
C855_rptC855ERR030012
C900_rptC900ERR030013
D075_rptD075ERR030014
D300_rptD300ERR030015
D492_rptD492ERR030005
D898_rptD898ERR030006
D909_rptD909ERR030007
B0403_rptB0403ERR031091
B0441_rptB0441ERR031095
B0442_rptB0442ERR031096
B0496_rptB0496ERR031097
B0583_rptB0583ERR031098
B0585_rptB0585ERR031099
B0881_rptB0881ERR031100
B0887_rptB0887ERR031101
B1865_rptB1865ERR031102
A214_rptA214ERR031092
E495_rptE495ERR031093
B0949_rptB0949ERR031094
Bp02-46_rptUnknownERR037419
Bp03-030_rptUnknownERR037423
Bp03-040_rptUnknownERR037424
Bp06-089_rptUnknownERR037425
Bp06-090_rptUnknownERR037426
Bp06-246_rptUnknownERR037427
Bp06-274_rptUnknownERR037428
Bp06-371_rptUnknownERR037429
Bp06-401_rptUnknownERR037430
UK1_rptUnknownERR037420
UK2_rptUnknownERR037421
UK3_rptUnknownERR037422
UK4_rptUnknownERR037396
UK5_rptUnknownERR037400
UK6_rptUnknownERR037401
UK8_rptUnknownERR037402
UK9_rptUnknownERR037403
UK10_rptUnknownERR037404
UK11_rptUnknownERR037405
UK14_rptUnknownERR037406
UK15_rptUnknownERR037407
UK16_rptUnknownERR037397
UK17_rptUnknownERR037398
UK18_rptUnknownERR037399
UK20_rptUnknownERR037408
UK21_rptUnknownERR037411
UK22_rptUnknownERR037412
UK23_rptUnknownERR037413
UK24_rptUnknownERR037414
bp4376_rptUnknownERR037415
bp4389_rptUnknownERR037416
bp4397_rptUnknownERR037417
bp4396_rptUnknownERR037418
bp4398_rptUnknownERR037409
bp4307_rptUnknownERR037410
  • The missing link: Bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic Bordetellae.

    Gross R, Guzman CA, Sebaihia M, dos Santos VA, Pieper DH, Koebnik R, Lechner M, Bartels D, Buhrmester J, Choudhuri JV, Ebensen T, Gaigalat L, Herrmann S, Khachane AN, Larisch C, Link S, Linke B, Meyer F, Mormann S, Nakunst D, Rückert C, Schneiker-Bekel S, Schulze K, Vorhölter FJ, Yevsa T, Engle JT, Goldman WE, Pühler A, Göbel UB, Goesmann A, Blöcker H, Kaiser O and Martinez-Arias R

    BMC genomics 2008;9;449

  • Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction.

    Sebaihia M, Preston A, Maskell DJ, Kuzmiak H, Connell TD, King ND, Orndorff PE, Miyamoto DM, Thomson NR, Harris D, Goble A, Lord A, Murphy L, Quail MA, Rutter S, Squares R, Squares S, Woodward J, Parkhill J and Temple LM

    Journal of bacteriology 2006;188;16;6002-15

  • Genomic and genetic analysis of Bordetella bacteriophages encoding reverse transcriptase-mediated tropism-switching cassettes.

    Liu M, Gingery M, Doulatov SR, Liu Y, Hodes A, Baker S, Davis P, Simmonds M, Churcher C, Mungall K, Quail MA, Preston A, Harvill ET, Maskell DJ, Eiserling FA, Parkhill J and Miller JF

    Journal of bacteriology 2004;186;5;1503-17

  • Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica.

    Parkhill J, Sebaihia M, Preston A, Murphy LD, Thomson N, Harris DE, Holden MT, Churcher CM, Bentley SD, Mungall KL, Cerdeño-Tárraga AM, Temple L, James K, Harris B, Quail MA, Achtman M, Atkin R, Baker S, Basham D, Bason N, Cherevach I, Chillingworth T, Collins M, Cronin A, Davis P, Doggett J, Feltwell T, Goble A, Hamlin N, Hauser H, Holroyd S, Jagels K, Leather S, Moule S, Norberczak H, O'Neil S, Ormond D, Price C, Rabbinowitsch E, Rutter S, Sanders M, Saunders D, Seeger K, Sharp S, Simmonds M, Skelton J, Squares R, Squares S, Stevens K, Unwin L, Whitehead S, Barrell BG and Maskell DJ

    Nature genetics 2003;35;1;32-40

Data Use Statement

This sequencing centre plans on publishing the completed and annotated sequences in a peer-reviewed journal as soon as possible. Permission of the principal investigator should be obtained before publishing analyses of the sequence/open reading frames/genes on a chromosome or genome scale. See our data sharing policy.

Sequencing enquiries

Please address all sequencing enquiries to: pathinfo@sanger.ac.uk

* quick link - http://q.sanger.ac.uk/9tln41nk