The Sanger Institute has been funded by the Wellcome Trust to sequence the genomes of Bordetella pertussis strain Tohama I, B. parapertussis strain 12822 and B. bronchiseptica strain RB50 in collaboration with Duncan Maskell and Andrew Preston of the Centre for Veterinary Science, Dept. of Clinical Veterinary medicine, The University of Cambridge. The sequences and analysis are described in: Parkhill et al (2003) Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica. Nature Genetics 35 32-40 (DOI: 10.1038/Ng1227), and have been submitted to EMBL/GenBank with the accession numbers: BX470248 (B. pertussis), BX470249 (B. parapertussis) and BX470250 (B. bronchiseptica).

The three sequenced Bordetella strains have been deposited with the ATCC and NCTC under the following accession numbers: Bordetella parapertussis 12822: ATCC BAA-587, NCTC 13253 Bordetella bronchiseptica RB50: ATCC BAA-588, NCTC 13252 Bordetella pertussis Tohama I: ATCC BAA-589, NCTC 13251

We have have also sequenced three closely related isolates of a B. pertussis bacteriophage that exhibited a novel mechanism of host range variation. The mechanism is described in Liu et al, Science 295, 2091 (2002), and the bacteriophage sequences were published in Liu et al, J Bacteriol 186, 1503-1517 (2004), and are available from Genbank/EMBL as: AY029185, AY526908 and AY526909.

We have also been funded by the USDA to sequence the genome of Bordetella avium strain 197N in collaboration with Louise Temple of the Department of Biology, James Madison University

Bordetella bronchiseptica complex sequencing

The Sanger Institute has initiated a project to sequence further strains within the Bordetella bronchiseptica complex to investigate the evolutionary history and host tropism differences within this group of organsims. The strain selection is based on the MLST tree published in Diavatopoulos et al, PLoS Pathog. 1, e45 (2005).

This work is being done in collaboration with Dr. Craig Cummings and Prof. David Relman (Relman Lab, Dept. of Microbiology and Immunology, SUMC, Stanford University), Prof. Eric Harvill (Centre for Infectious Disease Dynamics, Penn State University), Dr. Andrew Preston (Univeristy of Bristol), Dr. Frits Mooi (Laboratory for Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, Bilthoven, Netherlands) and Dr. Jeff F. Miller (Department of Micribiology, Immunology and Molecular Genetics, UCLA).

The strains currently selected for sequencing are : B. bronchiseptica strain 253 (ST27) B. pertussis strain 18323 (ST24) B. parapertussis (ovine) Bpp5 (ST16) B. bronchiseptica MO149 (ST15)

Further strains are likely to be selected from within the tree in the near future.

This project is funded by the Wellcome Trust.

Published Genome Data

The fully sequenced and annotated reference genomes are available from EMBL/GenBank as follows :-

BX470248 (B. pertussis Tohama I)

BX470249 (B. parapertussis12822)

BX470250 (B. bronchiseptica RB50).

AM167904 (Bordetella avium strain 197N)

Shotgun and assembly data from these projects are available from our FTP site.

• The missing link: Bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic Bordetellae.

  Gross R, Guzman CA, Sebaihia M, dos Santos VA, Pieper DH, Koebnik R, Lechner M, Bartels D, Buhrmester J, Choudhuri JV, Ebensen T, Gaigalat L, Herrmann S, Khachane AN, Larisch C, Link S, Linke B, Meyer F, Mormann S, Nakunst D, Rückert C, Schneiker-Bekel S, Schulze K, Vorhölter FJ, Yevsa T, Engle JT, Goldman WE, Pühler A, Göbel UB, Goesmann A, Blöcker H, Kaiser O and Martinez-Arias R

  BMC genomics2008;9;449

  PUBMED: 18826580; PMC: 2572626; DOI: 10.1186/1471-2164-9-449

• Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction.

  Sebaihia M, Preston A, Maskell DJ, Kuzmiak H, Connell TD, King ND, Orndorff PE, Miyamoto DM, Thomson NR, Harris D, Goble A, Lord A, Murphy L, Quail MA, Rutter S, Squares R, Squares S, Woodward J, Parkhill J and Temple LM

  Journal of bacteriology2006;188;16;6002-15

  PUBMED: 16885469; PMC: 1540077; DOI: 10.1128/JB.01927-05

• Genomic and genetic analysis of Bordetella bacteriophages encoding reverse transcriptase-mediated tropism-switching cassettes.

  Liu M, Gingery M, Doulatov SR, Liu Y, Hodes A, Baker S, Davis P, Simmonds M, Churcher C, Mungall K, Quail MA, Preston A, Harvill ET, Maskell DJ, Eiserling FA, Parkhill J and Miller JF

  Journal of bacteriology 2004;186;5;1503-17

  PUBMED: 14973019; PMC: 344406

• Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica.

  Parkhill J, Sebaihia M, Preston A, Murphy LD, Thomson N, Harris DE, Holden MT, Churcher CM, Bentley SD, Mungall KL, Cerdeño-Tárraga AM, Temple L, James K, Harris B, Quail MA, Achtman M, Atkin R, Baker S, Basham D, Bason N, Cherevach I, Chillingworth T, Collins M, Cronin A, Davis P, Doggett J, Feltwell T, Goble A, Hamlin N, Hauser H, Holroyd S, Jagels K, Leather S, Moule S, Norberczak H, O'Neil S, Ormond D, Price C, Rabbinowitsch E, Rutter S, Sanders M, Saunders D, Seeger K, Sharp S, Simmonds M, Skelton J, Squares R, Squares S, Stevens K, Unwin L, Whitehead S, Barrell BG and Maskell DJ

  Nature genetics 2003;35;1;32-40

  PUBMED: 12910271; DOI: 10.1038/ng1227