Thierry Voet's group focuses on developing methods that characterise the DNA and RNA in a single cell to enable the exploration of DNA-mutation, the genetic differences between cells in a person's body and the relation of this diversity to disease.
We are developing methods to determine all classes of genetic variants in the DNA of a single cell, as well as the RNA molecules the cell transcribes, using next-generation sequencing. We are applying our methods to study mutagenesis (the mechanism and rate of genetic mutation), somatic genetic heterogeneity (the degree of genetic difference between individual cells in a person's body) and their effect on disease. Using our methods, we are probing for the extent, nature and biology of cellular heterogeneity in health and disease.
Concurrent whole-genome haplotyping and copy-number profiling of single cells.
American journal of human genetics 2015;96;6;894-912
Combined Single-Cell Functional and Gene Expression Analysis Resolves Heterogeneity within Stem Cell Populations.
Cell stem cell 2015;16;6;712-24
G&T-seq: parallel sequencing of single-cell genomes and transcriptomes.
Nature methods 2015;12;6;519-22
SNES makes sense? Single-cell exome sequencing evolves.
Genome biology 2015;16;86
Analysis of the genetic phylogeny of multifocal prostate cancer identifies multiple independent clonal expansions in neoplastic and morphologically normal prostate tissue.
Nature genetics 2015;47;4;367-372
Spatial and temporal diversity in genomic instability processes defines lung cancer evolution.
Science (New York, N.Y.) 2014;346;6206;251-6
Single cell analysis of cancer genomes.
Current opinion in genetics & development 2014;24;82-91
Single cell segmental aneuploidy detection is compromised by S phase.
Molecular cytogenetics 2014;7;46
Single cell genomics: advances and future perspectives.
PLoS genetics 2014;10;1;e1004126
Mosaic copy number variation in human neurons.
Science (New York, N.Y.) 2013;342;6158;632-7
Single-cell paired-end genome sequencing reveals structural variation per cell cycle.
Nucleic acids research 2013;41;12;6119-38
Genome-wide copy number profiling of single cells in S-phase reveals DNA-replication domains.
Nucleic acids research 2013;41;6;e66
Next-generation sequencing of disseminated tumor cells.
Frontiers in oncology 2013;3;320
Preimplantation genetic diagnosis guided by single-cell genomics.
Genome medicine 2013;5;8;71
Alzheimer's disease: A protective mutation.
Microarray analysis of copy number variation in single cells.
Nature protocols 2012;7;2;281-310
Single-cell copy number variation detection.
Genome biology 2011;12;8;R80
Breakage-fusion-bridge cycles leading to inv dup del occur in human cleavage stage embryos.
Human mutation 2011;32;7;783-93
The human cleavage stage embryo is a cradle of chromosomal rearrangements.
Cytogenetic and genome research 2011;133;2-4;160-8
Chromosome instability is common in human cleavage-stage embryos.
Nature medicine 2009;15;5;577-83