Dr Anneliese Speak | Senior Staff Scientist

Speak, Anneliese

I am interested in how alterations in genes cause developmental or functional abnormalities in the immune system with a current focus on cancer immunology.

My work aims to further uncover the interaction between the immune system and developing tumours, with a long-term view to enable this interaction to be manipulated for the development of new therapies. This involves polychromatic flow cytometry (analysis and sorting), DNA and RNA sequencing plus in vitro and in vivo models to understand the function of genes in the immune system.

Previously while at the Sanger Institute, I managed a small team responsible for high-throughput immunophenotyping of knockout mice as part of mouse pipelines and was involved with the The Infection and Immunity Immunophenotyping (3i) consortium.

As a research associate at the University of Southern California I was investigating the link between viral infections, autophagy and the development of asthma.

Prior to this my first postdoctoral position and my DPhil studies were performed at Oxford University. Here I studied the impact of lysosomal storage in Niemman-Pick type C disease patients on the development and function of Natural Killer cells and invariant Natural Killer T cells. During this time I performed biomarker studies on blood samples from various lysosomal storage disorder patients from around the world. Using various biochemical techniques my DPhil studies involved invariant Natural Killer T cell ligands.

Publications

  • Disruption of the potassium channel regulatory subunit KCNE2 causes iron-deficient anemia.

    Salsbury G, Cambridge EL, McIntyre Z, Arends MJ, Karp NA et al.

    Experimental hematology 2014;42;12;1053-8.e1

  • Relative acidic compartment volume as a lysosomal storage disorder-associated biomarker.

    te Vruchte D, Speak AO, Wallom KL, Al Eisa N, Smith DA et al.

    The Journal of clinical investigation 2014;124;3;1320-8

  • Altered distribution and function of natural killer cells in murine and human Niemann-Pick disease type C1.

    Speak AO, Te Vruchte D, Davis LC, Morgan AJ, Smith DA et al.

    Blood 2014;123;1;51-60

  • Invariant natural killer T cells are not affected by lysosomal storage in patients with Niemann-Pick disease type C.

    Speak AO, Platt N, Salio M, te Vruchte D, Smith DA et al.

    European journal of immunology 2012;42;7;1886-92

  • CD8α⁺β⁻ and CD8α⁺β⁺ plasmacytoid dendritic cells induce Foxp3⁺ regulatory T cells and prevent the induction of airway hyper-reactivity.

    Lombardi V, Speak AO, Kerzerho J, Szely N and Akbari O

    Mucosal immunology 2012;5;4;432-43

  • Activation of invariant NKT cells by toll-like receptor 9-stimulated dendritic cells requires type I interferon and charged glycosphingolipids.

    Paget C, Mallevaey T, Speak AO, Torres D, Fontaine J et al.

    Immunity 2007;27;4;597-609

  • Implications for invariant natural killer T cell ligands due to the restricted presence of isoglobotrihexosylceramide in mammals.

    Speak AO, Salio M, Neville DC, Fontaine J, Priestman DA et al.

    Proceedings of the National Academy of Sciences of the United States of America 2007;104;14;5971-6

  • Normal development and function of invariant natural killer T cells in mice with isoglobotrihexosylceramide (iGb3) deficiency.

    Porubsky S, Speak AO, Luckow B, Cerundolo V, Platt FM and Gröne HJ

    Proceedings of the National Academy of Sciences of the United States of America 2007;104;14;5977-82

  • The AMP-activated protein kinase beta 1 subunit modulates erythrocyte integrity.

    Cambridge EL, McIntyre Z, Clare S, Arends MJ, Goulding D et al.

    Experimental hematology 2017;45;64-68.e5

  • Synthetic lethality between PAXX and XLF in mammalian development.

    Balmus G, Barros AC, Wijnhoven PW, Lescale C, Hasse HL et al.

    Genes & development 2016;30;19;2152-2157

  • Infection Susceptibility in Gastric Intrinsic Factor (Vitamin B12)-Defective Mice Is Subject to Maternal Influences.

    Mottram L, Speak AO, Selek RM, Cambridge EL, McIntyre Z et al.

    mBio 2016;7;3

  • Alkaline ceramidase 1 is essential for mammalian skin homeostasis and regulating whole body energy expenditure.

    Liakath-Ali K, Vancollie VE, Lelliott CJ, Speak AO, Lafont D et al.

    The Journal of pathology 2016

  • T cell fate and clonality inference from single-cell transcriptomes.

    Stubbington MJT, Lönnberg T, Proserpio V, Clare S, Speak AO et al.

    Nature methods 2016;13;4;329-332

  • Immune dysfunction in Niemann-Pick disease type C.

    Platt N, Speak AO, Colaco A, Gray J, Smith DA et al.

    Journal of neurochemistry 2016;136 Suppl 1;74-80

  • Gray platelet syndrome: proinflammatory megakaryocytes and α-granule loss cause myelofibrosis and confer metastasis resistance in mice.

    Guerrero JA, Bennett C, van der Weyden L, McKinney H, Chin M et al.

    Blood 2014;124;24;3624-35

  • Disruption of the potassium channel regulatory subunit KCNE2 causes iron-deficient anemia.

    Salsbury G, Cambridge EL, McIntyre Z, Arends MJ, Karp NA et al.

    Experimental hematology 2014;42;12;1053-8.e1

  • Complete humanization of the mouse immunoglobulin loci enables efficient therapeutic antibody discovery.

    Lee EC, Liang Q, Ali H, Bayliss L, Beasley A et al.

    Nature biotechnology 2014;32;4;356-63

  • Relative acidic compartment volume as a lysosomal storage disorder-associated biomarker.

    te Vruchte D, Speak AO, Wallom KL, Al Eisa N, Smith DA et al.

    The Journal of clinical investigation 2014;124;3;1320-8

  • Altered distribution and function of natural killer cells in murine and human Niemann-Pick disease type C1.

    Speak AO, Te Vruchte D, Davis LC, Morgan AJ, Smith DA et al.

    Blood 2014;123;1;51-60

  • Impact of temporal variation on design and analysis of mouse knockout phenotyping studies.

    Karp NA, Speak AO, White JK, Adams DJ, Hrabé de Angelis M et al.

    PloS one 2014;9;10;e111239

  • Programmed cell death ligand 2 regulates TH9 differentiation and induction of chronic airway hyperreactivity.

    Kerzerho J, Maazi H, Speak AO, Szely N, Lombardi V et al.

    The Journal of allergy and clinical immunology 2013;131;4;1048-57, 1057.e1-2

  • Lack of PD-L1 expression by iNKT cells improves the course of influenza A infection.

    Maazi H, Singh AK, Speak AO, Lombardi V, Lam J et al.

    PloS one 2013;8;3;e59599

  • Globosides but not isoglobosides can impact the development of invariant NKT cells and their interaction with dendritic cells.

    Porubsky S, Speak AO, Salio M, Jennemann R, Bonrouhi M et al.

    Journal of immunology (Baltimore, Md. : 1950) 2012;189;6;3007-17

  • CD8α⁺β⁻ and CD8α⁺β⁺ plasmacytoid dendritic cells induce Foxp3⁺ regulatory T cells and prevent the induction of airway hyper-reactivity.

    Lombardi V, Speak AO, Kerzerho J, Szely N and Akbari O

    Mucosal immunology 2012;5;4;432-43

  • Invariant natural killer T cells are not affected by lysosomal storage in patients with Niemann-Pick disease type C.

    Speak AO, Platt N, Salio M, te Vruchte D, Smith DA et al.

    European journal of immunology 2012;42;7;1886-92

  • A sensitive and specific LC-MS/MS method for rapid diagnosis of Niemann-Pick C1 disease from human plasma.

    Jiang X, Sidhu R, Porter FD, Yanjanin NM, Speak AO et al.

    Journal of lipid research 2011;52;7;1435-45

  • Diverse endogenous antigens for mouse NKT cells: self-antigens that are not glycosphingolipids.

    Pei B, Speak AO, Shepherd D, Butters T, Cerundolo V et al.

    Journal of immunology (Baltimore, Md. : 1950) 2011;186;3;1348-60

  • Invariant NKT cells in adrenoleukodystrophy patients and mice.

    Gautron AS, Giquel B, Beaudoin L, Autrusseau E, Speak A et al.

    Journal of neuroimmunology 2010;229;1-2;204-11

  • Invariant NKT cells reduce the immunosuppressive activity of influenza A virus-induced myeloid-derived suppressor cells in mice and humans.

    De Santo C, Salio M, Masri SH, Lee LY, Dong T et al.

    The Journal of clinical investigation 2008;118;12;4036-48

  • [Glycosphingolipids Gb3 and iGb3. In vivo roles in hemolytic-uremic syndrome and iNKT cell function].

    Porubsky S, Luckow B, Bonrouhi M, Speak A, Cerundolo V et al.

    Der Pathologe 2008;29 Suppl 2;297-302

  • Glycosphingolipid depletion in PC12 cells using iminosugars protects neuronal membranes from anti-ganglioside antibody mediated injury.

    Townson KH, Speak AO, Greenshields KN, Goodyear CS, Willison HJ and Platt FM

    Journal of neuroimmunology 2008;203;1;33-8

  • CD1d presentation of glycolipids.

    Speak AO, Cerundolo V and Platt FM

    Immunology and cell biology 2008;86;7;588-97

  • Beneficial effects of substrate reduction therapy in a mouse model of GM1 gangliosidosis.

    Elliot-Smith E, Speak AO, Lloyd-Evans E, Smith DA, van der Spoel AC et al.

    Molecular genetics and metabolism 2008;94;2;204-11

  • Modulation of human natural killer T cell ligands on TLR-mediated antigen-presenting cell activation.

    Salio M, Speak AO, Shepherd D, Polzella P, Illarionov PA et al.

    Proceedings of the National Academy of Sciences of the United States of America 2007;104;51;20490-5

  • Activation of invariant NKT cells by toll-like receptor 9-stimulated dendritic cells requires type I interferon and charged glycosphingolipids.

    Paget C, Mallevaey T, Speak AO, Torres D, Fontaine J et al.

    Immunity 2007;27;4;597-609

  • Implications for invariant natural killer T cell ligands due to the restricted presence of isoglobotrihexosylceramide in mammals.

    Speak AO, Salio M, Neville DC, Fontaine J, Priestman DA et al.

    Proceedings of the National Academy of Sciences of the United States of America 2007;104;14;5971-6

  • Normal development and function of invariant natural killer T cells in mice with isoglobotrihexosylceramide (iGb3) deficiency.

    Porubsky S, Speak AO, Luckow B, Cerundolo V, Platt FM and Gröne HJ

    Proceedings of the National Academy of Sciences of the United States of America 2007;104;14;5977-82

Speak, Anneliese
Anneliese's Timeline
2015

Moved to Experimental Cancer Genetics group

2012

Started at the Sanger Institute in the Mouse Genetics Project

2011

Moved to the University of Southern California as a Research associate

2007

Obtained DPhil in Biochemistry from University of Oxford

Started Postdoctoral work at University of Oxford

2004

Obtained MBioChem from University of Oxford