Dr Theo Sanderson | Postdoctoral Fellow

Sanderson, Theo

I work on several projects in malaria genetics, both at the bench and in silico, in three different parasite species. But these projects share one goal: to use new technologies to modify malaria parasite genomes at high scale, and thereby generate insights into the large portion of the Plasmodium genome that remains uncharacterised experimentally.

Plasmodium berghei

As part of the PlasmoGEM team I build the systems that assign knock-out phenotypes to genes based on barcode-sequencing data. We have now assigned blood-stage phenotypes to 2,578 P. berghei genes, and I am developing new approaches to understand the sexual stages of the lifecycle and the journey of the parasite through the mosquito and into the liver of the next human host.

Plasmodium knowlesi

P. knowlesi is a zoonotic parasite which infects humans in South East Asia. It is also a valuable model which is more genetically tractable than P. falciparum and allows culture in human erythrocytes. I was involved in the discovery that this species is made up of two sympatric groups with highly dimorphic genomes (it has since been shown that these represent species adapted to two different monkey hosts). I have brought human-adapted P. knowlesi into the PlasmoGEM programme by developing new vectors and optimising transfection approaches. We are now using this system to study the localisation of putative invasion genes at scale.

Plasmodium falciparum

During my PhD I felt that there was a need for a database to record the hundreds of gene disruption attempts scattered across the literature. PhenoPlasm is the result, with curated data for 369 P. falciparum genes, as well as data for other species derived from a variety of sources. I hope that this will be a valuable resource for the P. falciparum community.

Publications

  • Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes.

    Bushell E, Gomes AR, Sanderson T, Anar B, Girling G et al.

    Cell 2017;170;2;260-272.e8

  • PhenoPlasm: a database of disruption phenotypes for malaria parasite genes.

    Sanderson T and Rayner JC

    Wellcome open research 2017;2;45

  • Plasmodium knowlesi genome sequences from clinical isolates reveal extensive genomic dimorphism.

    Pinheiro MM, Ahmed MA, Millar SB, Sanderson T, Otto TD et al.

    PloS one 2015;10;4;e0121303

  • How synthetic biology will reconsider natural bioluminescence and its applications.

    Reeve B, Sanderson T, Ellis T and Freemont P

    Advances in biochemical engineering/biotechnology 2014;145;3-30

Sanderson, Theo
Theo's Timeline
2015

Post-doc at Sanger Institute

2011

Began a PhD at the Wellcome Trust Sanger Institute

2008

Began undergraduate study at the University of Cambridge