Quail, Michael Andrew
Senior Staff Scientist within the DNA pipelines Research and Development Group with extensive knowledge, experience and skills in NGS (Illumina, PacBio, Ion Torrent, Oxford Nanopore), library preparation, molecular biology and automation.
My role at Sanger is to enable our DNA pipelines to meet the requirements of the Institute and the scientists within it.
I am involved in,
Evaluation and optimisation of existing and emerging sequencing technologies.
Evaulation and optimisation of library preparation protcols. Developing applications and optimising to achieve cost savings, improved performance, and to enable them to be performed reliably within our high throughput sequencing teams.
Evaluation and optimisation of ancilliary equipment including liquid handling automation, to enable more efficient sample handling and processing.
Improved protocols for the illumina genome analyzer sequencing system.
Current protocols in human genetics 2009;Chapter 18;Unit 18.2
SASI-Seq: sample assurance Spike-Ins, and highly differentiating 384 barcoding for Illumina sequencing.
BMC genomics 2014;15;110
Direct sequencing of small genomes on the Pacific Biosciences RS without library preparation.
G&T-seq: parallel sequencing of single-cell genomes and transcriptomes.
Nature methods 2015;12;6;519-22
A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers.
BMC genomics 2012;13;341
Optimal enzymes for amplifying sequencing libraries.
Nature methods 2011;9;1;10-1
Optimizing Illumina next-generation sequencing library preparation for extremely AT-biased genomes.
BMC genomics 2012;13;1
A large genome center's improvements to the Illumina sequencing system.
Nature methods 2008;5;12;1005-10
Amplification-free Illumina sequencing-library preparation facilitates improved mapping and assembly of (G+C)-biased genomes.
Nature methods 2009;6;4;291-5