Matthew Mayho, PhD | Staff Scientist

Mayho, Matthew

Matt works within the DNA Pipelines Research and Development group where he helps to develop and implement novel protocols used by the operational sequencing teams. Matt’s skills and experience range from NGS library preparation and automation to troubleshooting sequencing quality issues.

I am currently working with the Sample Management team helping to improve their DNA quantification processes. This work is very collaborative and involves working closely with the team as well as automation specialists. We need to look at basic scientific principles such as the linearity of the fluorescent assay, and also the finer details of how well lab equipment is working and also changes to the LIMS system. Other recent projects I’ve been involved with have included developing a stranded mRNA-seq process for our Illumina Bespoke team and evaluating new and innovative pieces of laboratory automation equipment.
Since 2011 I have managed the sequencing side of the TraDIS project (transposon-directed insertion-site sequencing). TraDIS samples have proved challenging both in terms of library making and sequencing. Solving these issues has meant working closely with the pathogen researchers who generate the samples, as well as the sequencing technology specialists here at the Sanger Institute and Illumina.

Publications

  • High-throughput analysis of gene essentiality and sporulation in Clostridium difficile.

    Dembek M, Barquist L, Boinett CJ, Cain AK, Mayho M et al.

    mBio 2015;6;2;e02383

  • A conditional piggyBac transposition system for genetic screening in mice identifies oncogenic networks in pancreatic cancer.

    Rad R, Rad L, Wang W, Strong A, Ponstingl H et al.

    Nature genetics 2015;47;1;47-56

  • Generation of a Tn5 transposon library in Haemophilus parasuis and analysis by transposon-directed insertion-site sequencing (TraDIS).

    Luan SL, Chaudhuri RR, Peters SE, Mayho M, Weinert LA et al.

    Veterinary microbiology 2013;166;3-4;558-66

  • Evaluation and optimisation of preparative semi-automated electrophoresis systems for Illumina library preparation.

    Quail MA, Gu Y, Swerdlow H and Mayho M

    Electrophoresis 2012;33;23;3521-8

  • Comparative genomics of the emerging human pathogen Photorhabdus asymbiotica with the insect pathogen Photorhabdus luminescens.

    Wilkinson P, Waterfield NR, Crossman L, Corton C, Sanchez-Contreras M et al.

    BMC genomics 2009;10;302

  • Post-transcriptional control of nuclear-encoded cytochrome oxidase subunits in Trypanosoma brucei: evidence for genome-wide conservation of life-cycle stage-specific regulatory elements.

    Mayho M, Fenn K, Craddy P, Crosthwaite S and Matthews K

    Nucleic acids research 2006;34;18;5312-24

  • High-throughput analysis of gene essentiality and sporulation in Clostridium difficile.

    Dembek M, Barquist L, Boinett CJ, Cain AK, Mayho M et al.

    mBio 2015;6;2;e02383

  • A conditional piggyBac transposition system for genetic screening in mice identifies oncogenic networks in pancreatic cancer.

    Rad R, Rad L, Wang W, Strong A, Ponstingl H et al.

    Nature genetics 2015;47;1;47-56

  • Generation of a Tn5 transposon library in Haemophilus parasuis and analysis by transposon-directed insertion-site sequencing (TraDIS).

    Luan SL, Chaudhuri RR, Peters SE, Mayho M, Weinert LA et al.

    Veterinary microbiology 2013;166;3-4;558-66

  • Evaluation and optimisation of preparative semi-automated electrophoresis systems for Illumina library preparation.

    Quail MA, Gu Y, Swerdlow H and Mayho M

    Electrophoresis 2012;33;23;3521-8

  • Comparative genomics of the emerging human pathogen Photorhabdus asymbiotica with the insect pathogen Photorhabdus luminescens.

    Wilkinson P, Waterfield NR, Crossman L, Corton C, Sanchez-Contreras M et al.

    BMC genomics 2009;10;302

  • Post-transcriptional control of nuclear-encoded cytochrome oxidase subunits in Trypanosoma brucei: evidence for genome-wide conservation of life-cycle stage-specific regulatory elements.

    Mayho M, Fenn K, Craddy P, Crosthwaite S and Matthews K

    Nucleic acids research 2006;34;18;5312-24

Mayho, Matthew
Matthew's Timeline
2013

Becomes a development specialist with a focus on developing and troubleshooting protocols directly used by the operational teams

2011

Joined the Sequencing R&D team as a Staff Scientist

2007

Joined the Wellcome Trust Sanger Institute as an Advanced Research Assistant

2006

PhD University of Manchester ‘The cytochrome oxidase complex (COX) as a model for developmental gene expression in African trypanosomes’

2002

BSc Biological Sciences, University of Salford