Dr Cecile Crosnier | Senior Staff Scientist

Crosnier, Cecile

Cecile’s main interest is the identification of new interactions between proteins displayed at the cellular surface of pathogens and of their hosts. Her primary focus has been on two human parasitic diseases most prevalent in developing countries, malaria and schistosomiasis. The identification of interactions involved in parasite invasion of their host could help in the rational design of new vaccines and therapies.

I joined the Wright faculty lab in 2007 and have mostly been working on identifying new receptor-ligand interactions between pathogens and their host. To identify targets that can be easily accessible to therapeutics, we have been focusing on pathogen and host proteins that are displayed at the cell surface. My role is to compile, express and test large libraries of recombinant proteins that represent the cell surface repertoire of various pathogens.

I first focused on cell surface proteins displayed at the surface of merozoites, the blood-stage form of the Plasmodium falciparum parasite that causes malaria. Using a technique to detect low affinity extracellular interactions, I screened this panel of parasite proteins against a library of proteins present at the surface of the human red blood cell. Using this approach, I identified some new host-pathogen interactions, including the one between the P. falciparum RH5 and human basigin, which is essential for the invasion of P. falciparum into the human erythrocyte. RH5 has now become a primary target in the development of an effective vaccine against malaria.

More recently, I have begun a new project looking at extracellular proteins of Schistosoma mansoni, the parasite that causes bilharzia, for which no vaccine is available despite being the second most common parasitic disease. We have compiled a library proteins mostly expressed at the schistomula stage, which occurs shortly after the parasite has penetrated the human host’s skin. Using a systematic screening approach, we hope to identify new vaccine targets.

Publications

  • A PfRH5-based vaccine is efficacious against heterologous strain blood-stage Plasmodium falciparum infection in aotus monkeys.

    Douglas AD, Baldeviano GC, Lucas CM, Lugo-Roman LA, Crosnier C et al.

    Cell host & microbe 2015;17;1;130-9

  • New antigens for a multicomponent blood-stage malaria vaccine.

    Osier FH, Mackinnon MJ, Crosnier C, Fegan G, Kamuyu G et al.

    Science translational medicine 2014;6;247;247ra102

  • A library of functional recombinant cell-surface and secreted P. falciparum merozoite proteins.

    Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K et al.

    Molecular & cellular proteomics : MCP 2013;12;12;3976-86

  • Semaphorin-7A is an erythrocyte receptor for P. falciparum merozoite-specific TRAP homolog, MTRAP.

    Bartholdson SJ, Bustamante LY, Crosnier C, Johnson S, Lea S et al.

    PLoS pathogens 2012;8;11;e1003031

  • A full-length recombinant Plasmodium falciparum PfRH5 protein induces inhibitory antibodies that are effective across common PfRH5 genetic variants.

    Bustamante LY, Bartholdson SJ, Crosnier C, Campos MG, Wanaguru M et al.

    Vaccine 2013;31;2;373-9

  • Biochemical and functional analysis of two Plasmodium falciparum blood-stage 6-cys proteins: P12 and P41.

    Taechalertpaisarn T, Crosnier C, Bartholdson SJ, Hodder AN, Thompson J et al.

    PloS one 2012;7;7;e41937

  • Basigin is a receptor essential for erythrocyte invasion by Plasmodium falciparum.

    Crosnier C, Bustamante LY, Bartholdson SJ, Bei AK, Theron M et al.

    Nature 2011;480;7378;534-7

  • A rapid and scalable method for selecting recombinant mouse monoclonal antibodies.

    Crosnier C, Staudt N and Wright GJ

    BMC biology 2010;8;76

  • Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.

    Crosnier C, Vargesson N, Gschmeissner S, Ariza-McNaughton L, Morrison A and Lewis J

    Development (Cambridge, England) 2005;132;5;1093-104

  • Mutations in JAGGED1 gene are predominantly sporadic in Alagille syndrome.

    Crosnier C, Driancourt C, Raynaud N, Dhorne-Pollet S, Pollet N et al.

    Gastroenterology 1999;116;5;1141-8

  • New antigens for a multicomponent blood-stage malaria vaccine.

    Osier FH, Mackinnon MJ, Crosnier C, Fegan G, Kamuyu G et al.

    Science translational medicine 2014;6;247;247ra102

  • Naturally acquired antibodies specific for Plasmodium falciparum reticulocyte-binding protein homologue 5 inhibit parasite growth and predict protection from malaria.

    Tran TM, Ongoiba A, Coursen J, Crosnier C, Diouf A et al.

    The Journal of infectious diseases 2014;209;5;789-98

  • Cloning of recombinant monoclonal antibodies from hybridomas in a single mammalian expression plasmid.

    Müller-Sienerth N, Crosnier C, Wright GJ and Staudt N

    Methods in molecular biology (Clifton, N.J.) 2014;1131;229-40

  • Neutralization of Plasmodium falciparum merozoites by antibodies against PfRH5.

    Douglas AD, Williams AR, Knuepfer E, Illingworth JJ, Furze JM et al.

    Journal of immunology (Baltimore, Md. : 1950) 2014;192;1;245-58

  • A library of functional recombinant cell-surface and secreted P. falciparum merozoite proteins.

    Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K et al.

    Molecular & cellular proteomics : MCP 2013;12;12;3976-86

  • Biochemical analysis of the Plasmodium falciparum erythrocyte-binding antigen-175 (EBA175)-glycophorin-A interaction: implications for vaccine design.

    Wanaguru M, Crosnier C, Johnson S, Rayner JC and Wright GJ

    The Journal of biological chemistry 2013;288;45;32106-17

  • Identifying novel Plasmodium falciparum erythrocyte invasion receptors using systematic extracellular protein interaction screens.

    Bartholdson SJ, Crosnier C, Bustamante LY, Rayner JC and Wright GJ

    Cellular microbiology 2013;15;8;1304-12

  • A full-length recombinant Plasmodium falciparum PfRH5 protein induces inhibitory antibodies that are effective across common PfRH5 genetic variants.

    Bustamante LY, Bartholdson SJ, Crosnier C, Campos MG, Wanaguru M et al.

    Vaccine 2013;31;2;373-9

  • Biochemical and functional analysis of two Plasmodium falciparum blood-stage 6-cys proteins: P12 and P41.

    Taechalertpaisarn T, Crosnier C, Bartholdson SJ, Hodder AN, Thompson J et al.

    PloS one 2012;7;7;e41937

  • Semaphorin-7A is an erythrocyte receptor for P. falciparum merozoite-specific TRAP homolog, MTRAP.

    Bartholdson SJ, Bustamante LY, Crosnier C, Johnson S, Lea S et al.

    PLoS pathogens 2012;8;11;e1003031

  • Basigin is a receptor essential for erythrocyte invasion by Plasmodium falciparum.

    Crosnier C, Bustamante LY, Bartholdson SJ, Bei AK, Theron M et al.

    Nature 2011;480;7378;534-7

  • A rapid and scalable method for selecting recombinant mouse monoclonal antibodies.

    Crosnier C, Staudt N and Wright GJ

    BMC biology 2010;8;76

  • Endothelial signals modulate hepatocyte apicobasal polarization in zebrafish.

    Sakaguchi TF, Sadler KC, Crosnier C and Stainier DY

    Current biology : CB 2008;18;20;1565-71

  • Biological function of mutant forms of JAGGED1 proteins in Alagille syndrome: inhibitory effect on Notch signaling.

    Boyer-Di Ponio J, Wright-Crosnier C, Groyer-Picard MT, Driancourt C, Beau I et al.

    Human molecular genetics 2007;16;22;2683-92

  • Fgf10 regulates hepatopancreatic ductal system patterning and differentiation.

    Dong PD, Munson CA, Norton W, Crosnier C, Pan X et al.

    Nature genetics 2007;39;3;397-402

  • Organizing cell renewal in the intestine: stem cells, signals and combinatorial control.

    Crosnier C, Stamataki D and Lewis J

    Nature reviews. Genetics 2006;7;5;349-59

  • Expression of mutant JAGGED1 alleles in patients with Alagille syndrome.

    Boyer J, Crosnier C, Driancourt C, Raynaud N, Gonzales M et al.

    Human genetics 2005;116;6;445-53

  • Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.

    Crosnier C, Vargesson N, Gschmeissner S, Ariza-McNaughton L, Morrison A and Lewis J

    Development (Cambridge, England) 2005;132;5;1093-104

  • Alagille syndrome. The widening spectrum of arteriohepatic dysplasia.

    Crosnier C, Lykavieris P, Meunier-Rotival M and Hadchouel M

    Clinics in liver disease 2000;4;4;765-78

  • JAGGED1 gene expression during human embryogenesis elucidates the wide phenotypic spectrum of Alagille syndrome.

    Crosnier C, Attié-Bitach T, Encha-Razavi F, Audollent S, Soudy F et al.

    Hepatology (Baltimore, Md.) 2000;32;3;574-81

  • Mutations in JAGGED1 gene are predominantly sporadic in Alagille syndrome.

    Crosnier C, Driancourt C, Raynaud N, Dhorne-Pollet S, Pollet N et al.

    Gastroenterology 1999;116;5;1141-8

Crosnier, Cecile
Cecile's Timeline
2014

Begins another project focused on the identification of novel vaccine targets for the bilharzia parasite, Schistosoma mansoni

2008

Starts a research project looking for new extracellular protein interactions between the human red blood cell and the malaria parasite, Plasmodium falciparum

2007

Joins the Wellcome Trust Sanger Institute in the Cell Surface Signalling laboratory “Development of a new method for selecting recombinant mouse monoclonal antibodies”

2001

Post-doctoral fellow in the Vertebrate Development Laboratory, Cancer Research UK London Research Institute “Role of the Notch signalling pathway in intestinal cell differentiation”

PhD in Human Genetics “The role of the JAGGED1 gene in Alagille syndrome”, Universite Paris 7 Denis Diderot and Hopital de Bicetre

1997

Graduate degree in Human Genetics, Universite Paris 7 Denis Diderot