I manually improve the genome sequence of helminths as part of the Parasite Genomics Group - improving assemblies at the sequence level using software tools to close gaps and resolve mis-assemblies, and through the structural and functional curation of predicted genes models.
I joined the Sanger Institute as a finisher working on the Human Genome Project then other large genomes including zebrafish, mouse, pig, and tomato. In later years, I worked on finishing more problematic regions, alongside the coordination of projects from a broad range of sequencing collaborations.
The genome and life-stage specific transcriptomes of Globodera pallida elucidate key aspects of plant parasitism by a cyst nematode.
Genome biology 2014;15;3;R43
The genome and transcriptome of Haemonchus contortus, a key model parasite for drug and vaccine discovery.
Genome biology 2013;14;8;R88
The genomes of four tapeworm species reveal adaptations to parasitism.
The zebrafish reference genome sequence and its relationship to the human genome.
The tomato genome sequence provides insights into fleshy fruit evolution.
Chromosomal rearrangements maintain a polymorphic supergene controlling butterfly mimicry.
Genomic libraries: I. Construction and screening of fosmid genomic libraries.
Methods in molecular biology (Clifton, N.J.) 2011;772;37-58
Genomic libraries: II. Subcloning, sequencing, and assembling large-insert genomic DNA clones.
Methods in molecular biology (Clifton, N.J.) 2011;772;59-81
Characterization of a hotspot for mimicry: assembly of a butterfly wing transcriptome to genomic sequence at the HmYb/Sb locus.
Molecular ecology 2010;19 Suppl 1;240-54
The genomic sequence and analysis of the swine major histocompatibility complex.
The DNA sequence and biological annotation of human chromosome 1.
The DNA sequence and analysis of human chromosome 13.