Dr Louise Aigrain | Staff Scientist

Louise focusses her research on testing and optimising new DNA library preparation methods to support our Faculties, DNA sequencing pipelines, as well as external collaborators. Her main goal is to push the performances of Next Generation Sequencing platforms and to design novel bespoke protocols for challenging samples or projects.

Current work:

I joined the Sanger Institute as a staff Scientist in 2014. My role is to evaluate and optimise new DNA library preparation methods with the view to transfer them to our Sequencing Pipelines, and to design new bespoke protocols to support our Faculties and collaborators. Some of my current projects involve the development of a new library protocol for ancient DNA specifically for the HiSeqX platform, beta testing of the Oxford Nanopore Technology and assessing the efficiency of any new DNA library preparation kits.

Previous experience:

Before joining the Sanger Institute, I spent 3 years as a Post-Doctoral Research Assistant at the University of Oxford studying the dynamics of DNA polymerase I (PolI) using single molecules fluorescence microscopy both in vitro and in vivo. Previous to this, I completed a PhD in Biochemistry and Molecular Biology focussing on the structure-function relationship of the NADPH-Cytochrome P450 reductase (CPR) at the University Paris-Sud. These studies established and illustrated the role of domain motion both in PolI and CPR for their fidelity and efficiency. In order to perform in vivo observations, I was also involved in the development of a novel method to internalise labelled biomolecules in living micro-organisms.

This expertise in Biophysics and Biochemistry has equipped me with the relevant tools to tackle NGS technologies in the wet lab, as well as on the data analysis and hardware fronts.

Publications

  • Real-time single-molecule studies of the motions of DNA polymerase fingers illuminate DNA synthesis mechanisms.

    Evans GW, Hohlbein J, Craggs T, Aigrain L and Kapanidis AN

    Nucleic acids research 2015;43;12;5998-6008

  • Internalization and observation of fluorescent biomolecules in living microorganisms via electroporation.

    Aigrain L, Sustarsic M, Crawford R, Plochowietz A and Kapanidis AN

    Journal of visualized experiments : JoVE 2015;96

  • Long-lived intracellular single-molecule fluorescence using electroporated molecules.

    Crawford R, Torella JP, Aigrain L, Plochowietz A, Gryte K et al.

    Biophysical journal 2013;105;11;2439-50

  • Conformational landscapes of DNA polymerase I and mutator derivatives establish fidelity checkpoints for nucleotide insertion.

    Hohlbein J, Aigrain L, Craggs TD, Bermek O, Potapova O et al.

    Nature communications 2013;4;2131

  • Dynamic control of electron transfers in diflavin reductases.

    Aigrain L, Fatemi F, Frances O, Lescop E and Truan G

    International journal of molecular sciences 2012;13;11;15012-41

  • Role of the interface between the FMN and FAD domains in the control of redox potential and electronic transfer of NADPH-cytochrome P450 reductase.

    Aigrain L, Pompon D and Truan G

    The Biochemical journal 2011;435;1;197-206

  • Structure of the open conformation of a functional chimeric NADPH cytochrome P450 reductase.

    Aigrain L, Pompon D, Moréra S and Truan G

    EMBO reports 2009;10;7;742-7

  • Real-time single-molecule studies of the motions of DNA polymerase fingers illuminate DNA synthesis mechanisms.

    Evans GW, Hohlbein J, Craggs T, Aigrain L and Kapanidis AN

    Nucleic acids research 2015;43;12;5998-6008

  • Internalization and observation of fluorescent biomolecules in living microorganisms via electroporation.

    Aigrain L, Sustarsic M, Crawford R, Plochowietz A and Kapanidis AN

    Journal of visualized experiments : JoVE 2015;96

  • Optimized delivery of fluorescently labeled proteins in live bacteria using electroporation.

    Sustarsic M, Plochowietz A, Aigrain L, Yuzenkova Y, Zenkin N and Kapanidis A

    Histochemistry and cell biology 2014;142;1;113-24

  • Long-lived intracellular single-molecule fluorescence using electroporated molecules.

    Crawford R, Torella JP, Aigrain L, Plochowietz A, Gryte K et al.

    Biophysical journal 2013;105;11;2439-50

  • Conformational landscapes of DNA polymerase I and mutator derivatives establish fidelity checkpoints for nucleotide insertion.

    Hohlbein J, Aigrain L, Craggs TD, Bermek O, Potapova O et al.

    Nature communications 2013;4;2131

  • Dynamic control of electron transfers in diflavin reductases.

    Aigrain L, Fatemi F, Frances O, Lescop E and Truan G

    International journal of molecular sciences 2012;13;11;15012-41

  • The closed and compact domain organization of the 70-kDa human cytochrome P450 reductase in its oxidized state as revealed by NMR.

    Vincent B, Morellet N, Fatemi F, Aigrain L, Truan G et al.

    Journal of molecular biology 2012;420;4-5;296-309

  • Role of the interface between the FMN and FAD domains in the control of redox potential and electronic transfer of NADPH-cytochrome P450 reductase.

    Aigrain L, Pompon D and Truan G

    The Biochemical journal 2011;435;1;197-206

  • Structure of the open conformation of a functional chimeric NADPH cytochrome P450 reductase.

    Aigrain L, Pompon D, Moréra S and Truan G

    EMBO reports 2009;10;7;742-7

  • Cloning, purification, crystallization and preliminary X-ray analysis of a chimeric NADPH-cytochrome P450 reductase.

    Aigrain L, Pompon D, Truan G and Moréra S

    Acta crystallographica. Section F, Structural biology and crystallization communications 2009;65;Pt 3;210-2

Aigrain, Louise
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Louise's Timeline
2015

Staff Scientist – R&D Sequencing team, Wellcome Trust Sanger Institute.

2011

Post-doctoral research assistant in Biophysics – Gene Machines Group, University of Oxford.

2010

Ph. D. in Biochemistry and Molecular Biology, Laboratory of Membrane Protein Engineering – French National Centre of Scientific Research (CNRS) & University Paris-Sud 11.

2007

Master’s degree in Bioorganic and Bioinorganic Chemistry & “Magistère” in Molecular Physical Chemistry – University of Paris-Sud 11.

2005

Bachelor’s degree in Physical Chemistry – University of Paris-Sud 11.