Phenotyping Data for Ankrd13a (MHKL) Associated with 'homeostasis/metabolism' (MP:0005376)

Indirect Calorimetry: Standard Protocol
Indirect Calorimetry (week 12, male mice prioritised) [Mice were fed on High Fat Diet (21.4% crude fat content, Western RD, 829100, Special Diets Services) from 4 weeks of age]: Respiratory metabolic function is assessed using the LabMaster system (TSE-systems). Mice are housed individually in calorimetry cages for a period of approximately 21 hours including a 5 hour light phase acclimatisation period. This is followed consecutively by a 12 hour dark (19:30 - 07:30) and minimum 3 hour light phase. Cumulative food intake, activity (beam breaks), volume of oxygen consumed and volume of carbon dioxide produced are all measured. From this data the respiratory exchange ratio and energy expenditure are derived. Total VO2, VCO2, activity and energy expenditure refers to an average during the total time period. Water bottles are weighed before and after the experiment to assess water consumption.
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Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Male
Hom
population
parameter
Graph MP Annotation
Respiratory Exchange Ratio
VCO2
VO2
Energy Expenditure
Energy Expenditure/Animal
Glucose Tolerance (ip): Standard Protocol
ipGTT (week 13) [Mice were fed on High Fat Diet (21.4% crude fat content, Western RD, 829100, Special Diets Services) from 4 weeks of age]: Mice are fasted overnight (maximum of 16 hours) before the glucose tolerance test. A fasting blood sample is taken before a bolus of glucose is administered by intra-peritoneal injection. Blood samples are tested for glucose concentration (Accu-Check Aviva, Roche) at 15, 30, 60 and 120 minutes following the glucose administration, and data presented as plasma glucose concentration.
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Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Hom
Male
Hom
population
parameter
Graph MP Annotation
ip-GTT
Fasted Plasma Glucose
Stress Induced Hyperthermia: Core Temperature
Core Temperature (week 14) [Mice were fed on High Fat Diet (21.4% crude fat content, Western RD, 829100, Special Diets Services) from 4 weeks of age]: Rectal core body temperature is measured using a TH-5 thermometer with a RET-3 probe (Viking Medical).
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Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Hom
Male
Hom
population
parameter
Graph MP Annotation
Core Temperature
Plasma Chemistry: Fasted Plasma Chemistry (Avertin)
Fasted Plasma Chemistry Metabolic Panel (week 15) [Mice were fed on High Fat Diet (21.4% crude fat content, Western RD, 829100, Special Diets Services) from 4 weeks of age]: Mice are fasted overnight (maximum 16 hours) then terminally anaesthetised with avertin, after which blood is collected into Lithium/Heparin coated tubes via the retro-orbital sinus. The resulting plasma is analysed for glucose, triglycerides, cholesterol, high density lipoproteins, low density lipoproteins, non-esterified free fatty acids (NEFAC) and glycerol on an Olympus AU400. For a subset of genes, fructosamine is analysed using the AU400 and plasma insulin concentration is measured by ELISA and/or Mesoscale Discovery (MSD) array technology.
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Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Hom
Male
Hom
population
parameter
Graph MP Annotation
Glucose
Triglycerides
Cholesterol
High Density Lipoprotein
Low Density Lipoprotein
NEFAC
Glycerol
Insulin (ELISA)
Plasma Chemistry: Plasma Chemistry (Avertin)
Plasma Chemistry Panel (week 14) (Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning): Non-fasted mice are terminally anaesthetised with avertin, and blood is collected into Lithium/Heparin coated tubes via the retro-orbital sinus. Plasma is analysed for the following parameters on an Olympus AU400:
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Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Hom
Male
Hom
population
parameter
Graph MP Annotation
Sodium
Potassium
Chloride
Glucose
Triglycerides
Cholesterol
High Density Lipoprotein
Low Density Lipoprotein
NEFAC
Glycerol
Amylase
Alanine Aminotransferase
Alkaline Phosphatase
Creatine Kinase
Aspartate Aminotransferase
Total Bilirubin
Total Protein
Albumin
Creatinine
Urea
Calcium
Magnesium
Iron
Phosphate
Lactate Dehydrogenase
Uric Acid