Phenotyping Data for N6amt2 (MFTF) Associated with 'hematopoietic system' (MP:0005397)

Haematology (CBC): Haematology V2 (Ketamine / Xylazine)
Haematology (week 16) [Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning]: Non-fasted mice are terminally anaesthetised with ketamine/xylazine, and blood is collected into EDTA coated tubes via the retro-orbital sinus. Whole blood is analysed for the following parameters: White and red blood cell counts, mean corpuscular volume, haemoglobin, erythrocyte indices (haematocrit, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, red blood cell distribution width), platelet counts and mean platelet volume. All blood samples are analysed with an automatic haematology analyser using volume impedance principle (scilVet Animal Blood Counter, RAB 015 A Ind.F, 01.09.03).
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Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Hom
Male
Hom
population
parameter
Graph MP Annotation
White Blood Cell Count
Red Blood Cell Count
Mean Corpuscular Volume
Haemoglobin
Haematocrit
Mean Corpuscular Haemoglobin
Mean Corpuscular Haemoglobin Conc.
Red Blood Cell Distribution Width
Platelet Count
Mean Platelet Volume
Haematology (CBC): PBL flow cytometry assay 6 week Salmonella Challenge
Haematology analysis is performed at 6 weeks of age (Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning): Non-fasted unanaesthetised mice are restrained and blood is collected into EDTA coated tubes via the tail. Whole blood is analysed for the following parameters: White and red blood cell counts, mean corpuscular volume, haemoglobin, erythrocyte indices (haematocrit, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, red blood cell distribution width), platelet counts and mean platelet volume. All blood samples are analysed with an automatic haematology analyser using volume impedance principle (scilVet Animal Blood Counter, RAB 015 A Ind.F, 01.09.03).
view legend
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Hom
Male
Hom
population
parameter
Graph MP Annotation
White Blood Cell Count
Red Blood Cell Count
Mean Corpuscular Volume
Haemoglobin
Haematocrit
Mean Corpuscular Haemoglobin
Mean Corpuscular Haemoglobin Conc.
Red Blood Cell Distribution Width
Platelet Count
Mean Platelet Volume
Peripheral Blood Leukocytes: PBL flow cytometry assay 6 week LSR II whole blood
Peripheral blood leukocyte assay is performed at 6 weeks of age (Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning): Non-fasted unanaesthetised mice are restrained and blood is collected into EDTA coated tubes via the tail. Whole blood is stained with a titrated cocktail of antibodies including CD45, TCRab, TCRgd, CD161, CD4, CD8, CD19, CD25, CD44 and CD62L. Samples are fixed and red blood cells lysed prior to acquisition on a BD LSR II flow cytometer after running automated compensation using compbeads and BD FACSDiva software. Data is analysed using FlowJo after singlet doublet discrimination, a time gate is used to exclude HTS issues and leukocytes identified with a SSC and CD45 gate. Total T cells, alpha beta T cells, CD4+ alpha beta T cells, CD8+ alpha beta T cells, gamma delta T cells, NKT cells, NK cells and B cells are reported as percentage of leukocytes. CD4+ CD25+ regulatory alpha beta T cells, CD4+ CD44hi CD62Llo alpha beta T cells and CD8+ CD44hi CD62Llo alpha beta T cells are reported as percentage of parent. Using the white blood cell count obtained from the haematology analysis absolute cell counts are derived for each population and reported as cells/ul.
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Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Hom
Male
Hom
population
parameter
Graph MP Annotation
Total T cell percentage
Total T cell number
CD4+ ab T cell %
CD4+ ab Treg cell %
CD4+ ab Treg cell number
CD4+ ab Teff cell %
CD4+ ab Teff cell number
CD8+ ab T cell %
CD8+ ab Teff cell %
CD8+ ab Teff cell number
gd T cell %
gd T cell number
NKT cell percentage
NKT cell number
NK cell percentage
NK cell number
B cell percentage
B cell number
Peripheral Blood Leukocytes: PBL flow cytometry assay 16 week LSR II 2 panel whole blood
Peripheral blood leukocyte assay is performed at 16 weeks of age (Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning): Non-fasted mice are terminally anaesthetised with ketamine/xylazine, and blood is collected into EDTA coated tubes via the retro-orbital sinus. Whole blood is stained with two titrated cocktails of antibodies. Panel 1 containing CD45, TCRab, TCRgd, CD161, CD4, CD8, CD25, CD44, CD62L and KLRG1. Panel 2 containing CD45, CD19, IgD, Ly6B, Ly6G, Ly6C, CD11b and I-A/I-E. Samples are fixed and red blood cells lysed prior to acquisition on a BD LSR II flow cytometer after running automated compensation using compbeads and BD FACSDiva software. Data is analysed using FlowJo after singlet doublet discrimination, a time gate is used to exclude HTS issues and leukocytes identified with a SSC and CD45 gate. Total T cells, alpha beta T cells, CD4+ alpha beta T cells, CD8+ alpha beta T cells, gamma delta T cells, NKT cells, NK cells, B cells, monocytes, granulocytes and eosinophils are reported as percentage of leukocytes. CD4+ CD25+ regulatory alpha beta T cells, CD4+ CD44hi CD62Llo alpha beta T cells, CD4+ KLRG1+ alpha beta T cells, CD8+ CD44hi CD62Llo alpha beta T cells, CD8+ KLRG1+ alpha beta T cells, KLRG1+ NK cells, IgD+ B cells, Ly6Chi I-A/I-Elo monocytes and Ly6Clo I-A/I-Elo monocytes are reported as percentage of parent. Using the white blood cell count obtained from the haematology analysis absolute cell counts are derived for each population and reported as cells/ul.
view legend
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Hom
Male
Hom
population
parameter
Graph MP Annotation
Total T cell percentage
Total T cell number
CD4+ ab Treg cell %
CD4+ ab Treg cell number
CD4+ ab Teff cell %
CD4+ ab Teff cell number
CD8+ ab Teff cell %
CD8+ ab Teff cell number
gd T cell %
gd T cell number
NKT cell percentage
NKT cell number
NK cell percentage
NK cell number
B cell percentage
B cell number
IgD+ B cell %
IgD+ B cell number
Neutrophil percentage
Neutrophil number
Eosinophil percentage
Eosinophil number
Monocyte percentage
Monocyte number