Auditory Brainstem Response (ABR) measurement for Fbxo7tm1a(EUCOMM)WtsiMGP Select

Results Summary

  • ABR thresholds normal
  • Click-ABR waveforms normal

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean threshold is plotted (+/- population standard deviation). The green shaded area indicates the median threshold (and 2.5% to 97.5% reference range) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Superimposed waveforms recorded at 20dB and 50dB sensation level were plotted. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and were plotted as a function of dB sensation level. Means are plotted (over a 95% reference range for each parameter).

ABR Thresholds Summary

ABR Thresholds for Fbxo7<tm1a(EUCOMM)Wtsi> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MEBV22.1a M01112244 Fbxo7/Fbxo7(locked) Male 13.8 25 25 15 25 25 30
MEBV22.1b M01112245 Fbxo7/Fbxo7(locked) Male 13.8 25 35 20 25 30 35
MEBV22.1c M01112246 Fbxo7/Fbxo7(locked) Male 13.8 30 30 20 30 40 45
MEBV23.1b M01156919 Fbxo7/Fbxo7(locked) Male 13.6 20 20 20 10 25 25
Fbxo7/Fbxo7 Average 13.75 25 27.5 18.75 22.5 30 33.75
Fbxo7/Fbxo7 Standard Deviation 0.09 3.54 5.59 2.17 7.5 6.12 7.4
Fbxo7/Fbxo7 Standard Error 0.04 1.77 2.8 1.08 3.75 3.06 3.7
Controls
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MEQF14.1f M01158624 +/+(locked) Female 13.9 25 30 15 20 35 40
MEQF15.1d M01158603 +/+(locked) Female 13.6 25 30 10 10 30 40
MCKW56.3a M01106664 +/+(locked) Male 13.8 25 30 25 20 40 45
MCKW56.3b M01106665 +/+(locked) Male 13.8 25 30 15 15 40 50
MCRP43.3b M01156212 +/+(locked) Male 13.8 25 30 20 15 25 40
MCRP43.3d M01156214 +/+(locked) Male 13.8 25 30 15 25 35 45
MDKB26.2b M01035530 +/+(locked) Male 13.6 25 35 20 25 30 45
MDKB26.2c M01035531 +/+(locked) Male 13.6 25 35 15 25 30 35
MDKB26.2k M01035539 +/+(locked) Male 13.6 30 30 25 25 35 45
MDVQ23.2a M01192647 +/+(locked) Male 13.8 30 35 25 30 35 40
MDVQ23.2d M01192650 +/+(locked) Male 13.8 25 25 35 25 30 40
+/+ Average 13.74 25.91 30.91 20 21.36 33.18 42.27
+/+ Standard Deviation 0.11 1.93 2.87 6.74 5.68 4.41 3.91
+/+ Standard Error 0.03 .58 .87 2.03 1.71 1.33 1.18

Waveform Overlay 20dB SL

Waveform Overlay 50dB SL