Phenotyping Data for Fbxo47 (MDTZ) Associated with 'homeostasis/metabolism' (MP:0005376)

Indirect Calorimetry: Standard Protocol
Indirect Calorimetry (week 12, male mice prioritised) [Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning]: Respiratory metabolic function is assessed using the LabMaster system (TSE-systems). Mice are housed individually in calorimetry cages for a period of approximately 21 hours including a 5 hour light phase acclimatisation period. This is followed consecutively by a 12 hour dark (19:30 - 07:30) and minimum 3 hour light phase. Cumulative food intake, activity (beam breaks), volume of oxygen consumed and volume of carbon dioxide produced are all measured. From this data the respiratory exchange ratio and energy expenditure are derived. Total VO2, VCO2, activity and energy expenditure refers to an average during the total time period. Water bottles are weighed before and after the experiment to assess water consumption.
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Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Male
Het
Male
Hom
population
parameter
Graph MP Annotation
Respiratory Exchange Ratio
VCO2
VO2
Energy Expenditure
Energy Expenditure/Animal
Glucose Tolerance (ip): IPGTT No Maltose Interference
ipGTT (week 13) [Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning]: Mice are fasted overnight (maximum of 18 hours) before the glucose tolerance test. A fasting blood sample is taken before a bolus of glucose is administered by intra-peritoneal injection. Blood samples are tested for glucose concentration (Accu-Check Aviva, Roche) at 15, 30, 60 and 120 minutes following the glucose administration, using strips modified such that there is no interference with maltose. Data are presented as plasma glucose concentration.
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Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Female
Hom
Male
Het
Male
Hom
population
parameter
Graph MP Annotation
ip-GTT
Fasted Plasma Glucose
Plasma Chemistry: Plasma Chemistry (Ketamine / Xylazine)
Comprehensive Clinical Chemistry Panel (week 16) [Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning]: Non-fasted mice are terminally anaesthetised with ketamine/xylazine, and blood is collected into Lithium/Heparin coated tubes via the retro-orbital sinus. Plasma is analysed for the following parameters on an Olympus AU400:
Electrolytes: Sodium, potassium, chloride
Non-Fasted Metabolic Panel: Glucose, fructosamine, triglycerides, cholesterol, high density lipoprotein, low density lipoprotein, non-esterified free fatty acids (NEFAC) and glycerol
Pancreatic Enzyme: Amylase
Liver/Muscle Panel: Alanine aminotransferase, alkaline phosphatase, creatine kinase, aspartate aminotransferase, total bilirubin
Protein Parameters: Total protein, albumin
Kidney Panel: Creatinine, urea
Minerals and Iron: Calcium, magnesium, iron, phosphate For a subset of genes, plasma lactate dehydrogenase and uric acid are analysed.
Plasma insulin concentration is measured by Mesoscale Discovery (MSD) array technology.
view legend
Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Female
Hom
Male
Het
Male
Hom
population
parameter
Graph MP Annotation
Sodium
Potassium
Chloride
Glucose
Fructosamine
Triglycerides
Cholesterol
High Density Lipoprotein
Low Density Lipoprotein
NEFAC
Glycerol
Insulin (MSD)
Total Thyroxine
Amylase
Alanine Aminotransferase
Alkaline Phosphatase
Creatine Kinase
Aspartate Aminotransferase
Total Bilirubin
Total Protein
Albumin
Creatinine
Urea
Calcium
Magnesium
Iron
Phosphate