Phenotyping Data for Fam63a (MDFC) Associated with 'immune system' (MP:0005387)

Salmonella Challenge: Standard Protocol
For each mutant line, 8 male mutant mice plus 8 male wildtype control mice are infected i.v. with 0.2ml Salmonella Typhimurium TETc at 6 weeks of age and are monitored over 28 days. On day 14 post infection, 4 of the mutant mice and 4 wildtype control mice are schedule 1 killed. The spleen, liver and caecal contents are extracted and bacteria enumerated by serial dilution and plating onto agar plates. In addition 1 lobe of liver and 1/3 of the spleen will be fixed in formaldehyde for histology. On day 28 post infection the remaining mice are bled under terminal anaesthesia via cardiac puncture. Organs are removed and processed in the same way as describe for day 14 post infection. Blood serum is tested for anti TETc specific antibodies by ELISA assay (IgG, IgG1 and IgG2a). Mice are weighed daily throughout the infection.
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Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Male
Hom
population
parameter
Graph MP Annotation
Spleen view graph
Liver view graph
Ceacal contents view graph
Citrobacter Challenge: Standard Protocol - significant parameters
For each mutant line, 8 female mutant mice plus 8 female wildtype control mice are infected orally with 0.2ml Citrobacter rodentium lux 109 at 6 weeks of age and are monitored over 28 days. Every 2 to 3 days faeces is collected from each mouse and the amount of bacteria in the faeces is enumerated by serial dilutions and plating onto agar plates. Every 3 to 4 days the mice may be imaged under a bioluminescence camera for 5 minutes. At day 14 post infection, 4 mutant and 4 wildtypes are schedule 1 killed. The spleen, liver, caecal patch, caecum, caecal contents and the last 6 cm of colon are extracted and the amount of bacteria in the tissue/contents is enumerated by serial dilution and plating onto agar plates. In addition, the 6 cm of colon is weighed to look for signs of gross hyperplasia, and the last 0.5 cm of most distal colon is removed and fixed for histology. At day 28 post infection the remaining mice are schedule 1 killed and the day 14 post infection procedures repeated. Mice are weighed daily throughout the infection.
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Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Hom
population
parameter
Graph MP Annotation
Bacterial Shedding view graph
Ceacal Patch view graph
Ceacum view graph
Ceacal Contents view graph
Colon view graph MP:0002412 - increased susceptibility to bacterial infection
Spleen view graph
Liver view graph
Colon Weight view graph MP:0002412 - increased susceptibility to bacterial infection
Tissue Biobank: Standard Protocol
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Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter population
parameter
Graph MP Annotation
Tissue Biobank