Auditory Brainstem Response (ABR) measurement for Alg13tm1a(KOMP)WtsiMGP Select

Results Summary

  • ABR thresholds normal
  • Click-ABR waveforms normal

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean threshold is plotted (+/- population standard deviation). The green shaded area indicates the median threshold (and 2.5% to 97.5% reference range) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Superimposed waveforms recorded at 20dB and 50dB sensation level were plotted. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and were plotted as a function of dB sensation level. Means are plotted (over a 95% reference range for each parameter).

ABR Thresholds Summary

ABR Thresholds for Alg13<tm1a(KOMP)Wtsi> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MCWY29.2b M01007886 Alg13/Alg13(locked) Female 14.3 25 25 20 25 35 50
MCWY29.2d M01007888 Alg13/Alg13(locked) Female 14.3 30 35 20 25 35 45
MCWY31.1c M01010691 Alg13/Alg13(locked) Female 13.9 25 30 15 15 30 50
MCWY31.1d M01010692 Alg13/Alg13(locked) Female 13.9 25 35 20 25 30 45
Alg13/Alg13 Average 14.1 26.25 31.25 18.75 22.5 32.5 47.5
Alg13/Alg13 Standard Deviation 0.2 2.17 4.15 2.17 4.33 2.5 2.5
Alg13/Alg13 Standard Error 0.1 1.08 2.07 1.08 2.17 1.25 1.25
MCWY38.1b M01067269 Alg13/Y(locked) Male 14.1 25 30 10 15 25 30
MCWY38.2c M01112985 Alg13/Y(locked) Male 13.6 30 40 25 30 40 70
MCWY39.1a M01070347 Alg13/Y(locked) Male 13.6 25 25 15 20 30 40
MCWY39.1c M01070349 Alg13/Y(locked) Male 13.6 30 35 15 30 25 40
Alg13/Y Average 13.73 27.5 32.5 16.25 23.75 30 45
Alg13/Y Standard Deviation 0.22 2.5 5.59 5.45 6.5 6.12 15
Alg13/Y Standard Error 0.11 1.25 2.8 2.72 3.25 3.06 7.5
Controls
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MCUB58.3e M01041137 +/+(locked) Female 14.1 25 30 10 15 30 55
MCUB58.3g M01041139 +/+(locked) Female 14.1 30 30 20 20 25 45
MCWC64.2g M01007896 +/+(locked) Female 14.3 20 25 15 15 30 40
MCWC64.2i M01007898 +/+(locked) Female 14.3 25 35 15 20 30 50
MEBY21.2g M01057833 +/+(locked) Female 14.2 20 35 20 15 30 35
MEBY21.2h M01057834 +/+(locked) Female 14.2 25 35 20 25 35 45
MEBY21.2i M01057835 +/+(locked) Female 14.2 25 30 15 20 30 25
MEDY27.1b M01057486 +/+(locked) Female 14.2 25 35 15 25 35 40
MAXZ48.1b M01039879 +/+(locked) Male 14.1 30 30 15 20 35 45
MAXZ48.1c M01039880 +/+(locked) Male 14.1 25 25 15 15 30 45
MCUB64.1d M01114597 +/+(locked) Male 14.3 20 45 30 20 25 40
MCUB65.1b M01114587 +/+(locked) Male 14.3 25 30 10 20 30 40
MCZN91.1a M01074052 +/+(locked) Male 14.2 20 40 20 10 25 30
MCZN91.1c M01074054 +/+(locked) Male 14.2 30 45 15 30 30 35
+/+ Average 14.2 24.64 33.57 16.79 19.29 30 40.71
+/+ Standard Deviation 0.08 3.52 6.1 4.86 4.95 3.27 7.53
+/+ Standard Error 0.02 .94 1.63 1.3 1.32 .87 2.01

Waveform Overlay 20dB SL

Waveform Overlay 50dB SL

Input Output Functions