Adult LacZ Expression Data for Spns2 (MCSR)

Overview

Adult expression (≥ 6 weeks): The lacZ reporter gene within the targeting vector was used to determine the wholemount expression profile of the targeted gene. Mice were fixed by cardiac perfusion using 4% paraformaldehyde (PFA). Following dissection, the tissues to be stained were fixed an additional 30 min in 4% PFA before being rinsed in phosphate buffered saline and transferred to lacZ staining solution, containing 0.1% X-gal, for up to 48 hours. After an additional overnight post-staining fixation in 4% PFA, tissues were cleared with 50% glycerol then transferred to 70% glycerol for long-term storage.

Display Criteria

A tick-list of 41 tissues and organs was scored for the presence (green tick) or absence (red cross) of lacZ expression.

  • The term 'Ambiguous' was assigned when there was uncertainty as to the validity of the observed pattern e.g. staining may be very faint, background or an artefact due to trapping.
  • The term 'No Data' was assigned if the stained entity was not available to assess.

By default, up to 27 standard images may be taken using a Leica MZ16A microscope and Imagic software. These images were only collected when X-gal staining was present.

If information or images that you are interested in are not presented, please send details to MGPenquiries@sanger.ac.uk and, on a limited, first come first served basis, we will process your request.

Tick-List
Colony Name Spns2 tm1b(KOMP)Wtsi Spns2 tm1b(KOMP)Wtsi Spns2 tm1b(KOMP)Wtsi Spns2 tm1b(KOMP)Wtsi Spns2 tm1b(KOMP)Wtsi Spns2 tm1b(KOMP)Wtsi Spns2 tm1b(KOMP)Wtsi
Genotype Spns2(b)/+ Spns2(b)/+ Spns2(b)/Spns2(b) Spns2(b)/+ Spns2(b)/+ Spns2(b)/+ Spns2(b)/+
Gender Male Female Female Male Female Male Female
Age In Weeks 8.8 8.8 11.1 7.1 3.8 6.7 6.7
Adrenal Gland absent absent absent absent absent absent absent
Bone present present present present present present present
Brain present present present present present present present
Brown Adipose Tissue absent absent absent absent absent absent absent
Cartilage absent absent ambiguous absent absent absent absent
Colon ambiguous absent present ambiguous absent ambiguous ambiguous
Eye present present present present present present present
Gall Bladder absent absent present absent absent absent absent
Heart absent absent present absent absent absent absent
Kidney present present present present present present present
Large Intestine ambiguous absent present ambiguous absent ambiguous ambiguous
Liver present present present present absent present present
Lung present present present present absent present present
Mammary Gland absent present present ambiguous present ambiguous present
Mesenteric Lymph Node present present present present present present present
Nasal Epithelia No data No data No data No data No data No data No data
Oesophagus present present present present present present present
Oral Epithelia present present present present present present present
Ovaries No data present present No data absent No data present
Oviduct No data present present No data present No data present
Pancreas present present present present absent present present
Parathyroid absent absent present present absent absent absent
Peripheral Nervous System present present present present present present present
Peyers Patch present present present present present present present
Pituitary Gland absent absent absent absent No data absent absent
Prostate ambiguous No data No data absent No data absent No data
Skeletal Muscle absent absent absent absent absent absent absent
Skin present present present present present present present
Small Intestine ambiguous absent present present absent ambiguous ambiguous
Spinal Cord present present present present present present present
Spleen present present present present absent present present
Stomach present present present present present present present
Testis present No data No data present No data present No data
Thymus present present present ambiguous absent absent absent
Thyroid ambiguous ambiguous present present absent absent ambiguous
Trachea absent absent present present absent absent absent
Urinary System present present present present present present present
Uterus No data present present No data absent No data present
Vas Deferens absent No data No data absent No data absent No data
Vascular System absent absent absent absent absent absent absent
White Adipose Tissue absent absent absent absent absent absent absent
Images
  • Tissue: Bladder
    Description: -
    Genotype: Heterozygous
  • Tissue: Brain
    Description: Longitudinal section
    Genotype: Heterozygous
  • Tissue: Brain
    Description: Lateral view
    Genotype: Heterozygous
  • Tissue: Hind limb
    Description: -
    Genotype: Heterozygous
  • Tissue: Kidney
    Description: Longitudinal section
    Genotype: Heterozygous
  • Tissue: Liver and gall bladder
    Description: -
    Genotype: Heterozygous
  • Tissue: Mesenteric lymph node
    Description: -
    Genotype: Heterozygous
  • Tissue: Mesenteric lymph node
    Description: -
    Genotype: Heterozygous
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Heterozygous
  • Tissue: Peyer's patch
    Description: -
    Genotype: Heterozygous
  • Tissue: Rib cage
    Description: -
    Genotype: Heterozygous
  • Tissue: Skin
    Description: -
    Genotype: Heterozygous
  • Tissue: Skin
    Description: -
    Genotype: Heterozygous
  • Tissue: Skull
    Description: -
    Genotype: Heterozygous
  • Tissue: Spleen
    Description: Cross-section
    Genotype: Heterozygous
  • Tissue: Stomach
    Description: -
    Genotype: Heterozygous
  • Tissue: Tail
    Description: -
    Genotype: Heterozygous
  • Tissue: Testis
    Description: -
    Genotype: Heterozygous
  • Tissue: Thymus
    Description: -
    Genotype: Heterozygous
  • Tissue: Urogenital system
    Description: -
    Genotype: Heterozygous
Background LacZ Staining in Adult

The adult wholemount LacZ expression protocol has been refined to minimize background staining however, it can still occur. We have systematically documented the sites and intensities of this background stain in male and female wildtype C57BL/6 (C57BL/6J-Tyr<c-Brd>; C57BL/6NTac/Den) mice. The major locations where background staining may be observed are:

  • Nasal cavity
  • Thyroid gland
  • Glandular stomach
  • Kidney
  • Male genitals

Additional background staining is observed occasionally in:

  • Rib cage
  • Thymus
  • Mesenteric lymph nodes
  • Ovaries

Examples of the pattern and level of background stain are presented below.

  • Tissue: Nasal cavity
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Nasal cavity
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Kidney
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Kidney
    Description: -
    Genotype: Wildtype
  • Tissue: Urogenital system
    Description: -
    Genotype: Wildtype
  • Tissue: Testis
    Description: -
    Genotype: Wildtype
  • Tissue: Rib cage
    Description: -
    Genotype: Wildtype
  • Tissue: Thymus
    Description: -
    Genotype: Wildtype
  • Tissue: Mesenteric lymph node
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype