Auditory Brainstem Response (ABR) measurement for Aff3tm1a(EUCOMM)WtsiMGP Select

Results Summary

  • In 5 out of 10 homs:
  • ABR thresholds elevated at high frequencies (24-30kHz)
  • Possible new mutation affecting hearing, unlinked to the targetted allele

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean threshold is plotted (+/- population standard deviation). The green shaded area indicates the median threshold (and 2.5% to 97.5% reference range) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Superimposed waveforms recorded at 20dB and 50dB sensation level were plotted. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and were plotted as a function of dB sensation level. Means are plotted (over a 95% reference range for each parameter).

ABR Thresholds Summary

ABR Thresholds for Aff3<tm1a(EUCOMM)Wtsi> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MCND45.2a M00993274 Aff3/+(locked) Male 14.2 25 35 15 15 30 45
MCND47.2a M00993278 Aff3/+(locked) Male 14.1 25 30 5 15 35 35
MCND47.2b M00993279 Aff3/+(locked) Male 14.1 25 30 15 20 30 45
MCND47.2c M00993280 Aff3/+(locked) Male 14.1 20 40 20 20 25 35
Aff3/+ Average 14.13 23.75 33.75 13.75 17.5 30 40
Aff3/+ Standard Deviation 0.04 2.17 4.15 5.45 2.5 3.54 5
Aff3/+ Standard Error 0.02 1.08 2.07 2.72 1.25 1.77 2.5
MCND51.3f M01087550 Aff3/Aff3(locked) Female 13.8 40 35 20 35 95 95
MCND51.3h M01087552 Aff3/Aff3(locked) Female 13.8 35 50 30 30 35 45
MCND51.4c M01127510 Aff3/Aff3(locked) Female 14.3 40 45 25 35 90 90
MCND51.4d M01127511 Aff3/Aff3(locked) Female 14.3 55 50 45 45 55 60
MCND51.4e M01127512 Aff3/Aff3(locked) Female 14.3 25 35 20 20 30 30
MCND49.1c M01024260 Aff3/Aff3(locked) Male 14.3 30 45 25 25 35 45
MCND49.3a M01087975 Aff3/Aff3(locked) Male 14.1 30 35 25 30 85 95
MCND58.2a M01257339 Aff3/Aff3(locked) Male 14.2 35 50 35 35 85 95
MCND58.2c M01257341 Aff3/Aff3(locked) Male 14.2 30 40 25 25 30 30
MCND58.2d M01257342 Aff3/Aff3(locked) Male 14.2 25 30 20 20 35 45
Aff3/Aff3 Average 14.15 34.5 41.5 27 30 57.5 63
Aff3/Aff3 Standard Deviation 0.19 8.5 7.09 7.48 7.42 26.48 26.38
Aff3/Aff3 Standard Error 0.06 2.69 2.24 2.37 2.35 8.37 8.34
Controls
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MCEC67.3c M01095069 +/+(locked) Female 14.1 25 30 20 15 30 40
MCEC67.3d M01095070 +/+(locked) Female 14.1 25 30 20 15 25 30
MCRQ85.2g M01264804 +/+(locked) Female 14.2 25 35 15 20 25 35
MCRQ85.2j M01264807 +/+(locked) Female 14.2 25 25 15 25 30 45
MCSC52.2c M01127542 +/+(locked) Female 14.2 35 30 25 25 30 55
MCSC52.2d M01127543 +/+(locked) Female 14.2 30 30 15 30 30 40
MCVN47.2b M01049597 +/+(locked) Female 14.1 25 40 20 20 30 50
MCVN47.2e M01049600 +/+(locked) Female 14.1 25 40 15 20 30 40
MDFF35.1c M01181943 +/+(locked) Female 13.6 25 35 15 25 30 35
MDFF36.1e M01181663 +/+(locked) Female 13.8 30 30 15 20 25 40
MDLQ22.1f M01073292 +/+(locked) Female 14.3 20 35 15 15 25 35
MDLQ22.1i M01073295 +/+(locked) Female 14.3 25 25 15 20 25 30
MAST79.1c M01096634 +/+(locked) Male 14.1 25 35 20 15 25 50
MAST79.1d M01096635 +/+(locked) Male 14.1 25 35 20 20 25 45
MDAH36.1a M00997932 +/+(locked) Male 13.8 25 30 15 10 20 35
MDAH36.1b M00997933 +/+(locked) Male 13.8 20 30 20 20 35 35
MDEF23.2a M01024841 +/+(locked) Male 13.9 25 40 15 10 35 40
MDEF23.2c M01024843 +/+(locked) Male 13.9 30 40 20 20 35 40
MECU17.1a M01060339 +/+(locked) Male 13.9 30 40 15 25 40 45
MECU17.1b M01060340 +/+(locked) Male 13.9 25 35 20 20 30 35
MEGB20.1a M01266135 +/+(locked) Male 14.2 25 35 15 25 30 40
MELE21.1c M01266113 +/+(locked) Male 14.2 25 35 20 25 30 30
+/+ Average 14.05 25.91 33.64 17.5 20 29.09 39.55
+/+ Standard Deviation 0.19 3.25 4.57 2.92 5 4.43 6.56
+/+ Standard Error 0.04 .69 .97 .62 1.07 .94 1.4

Waveform Overlay 20dB SL

Waveform Overlay 50dB SL