Auditory Brainstem Response (ABR) measurement for 2310007B03Riktm1a(KOMP)WtsiMouse GP

Results Summary

  • ABR thresholds normal
  • Click-ABR waveforms normal
  • Possible new mutation affecting hearing, unlinked to the targetted allele

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean threshold is plotted (+/- population standard deviation). The green shaded area indicates the median threshold (and 2.5% to 97.5% reference range) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Superimposed waveforms recorded at 20dB and 50dB sensation level were plotted. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and were plotted as a function of dB sensation level. Means are plotted (over a 95% reference range for each parameter).

ABR Thresholds Summary

ABR Thresholds for 2310007B03Rik<tm1a(KOMP)Wtsi> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MCBX16.1b M00438493 2310007B03Rik/2310007B03Rik(locked) Female 14.2 25 35 15 20 25 35
MCBX16.1e M00438496 2310007B03Rik/2310007B03Rik(locked) Female 14.2 60 90 70 65 75 70
MCBX16.1f M00438497 2310007B03Rik/2310007B03Rik(locked) Female 14.2 60 85 70 65 60 60
MCBX16.2c M00461449 2310007B03Rik/2310007B03Rik(locked) Female 13.9 20 40 20 20 35 40
MCBX16.2d M00461450 2310007B03Rik/2310007B03Rik(locked) Female 13.9 50 90 75 70 75 55
MCBX16.2e M00461451 2310007B03Rik/2310007B03Rik(locked) Female 13.9 25 30 10 15 25 60
MCBX16.2f M00461452 2310007B03Rik/2310007B03Rik(locked) Female 13.9 55 95 80 70 70 70
MCBX23.1b M00527816 2310007B03Rik/2310007B03Rik(locked) Female 13.9 25 25 10 20 30 40
MCBX11.2b M00438463 2310007B03Rik/2310007B03Rik(locked) Male 14.2 45 80 70 40 40 45
MCBX24.2a M00559730 2310007B03Rik/2310007B03Rik(locked) Male 14.1 25 30 15 15 20 30
MCBX25.1a M00527821 2310007B03Rik/2310007B03Rik(locked) Male 14.2 25 30 15 20 25 55
MCBX25.1b M00527822 2310007B03Rik/2310007B03Rik(locked) Male 14.2 25 35 20 25 35 40
MCBX25.1c M00527823 2310007B03Rik/2310007B03Rik(locked) Male 14.2 25 45 25 25 30 40
2310007B03Rik/2310007B03Rik Average 14.08 35.77 54.62 38.08 36.15 41.92 49.23
2310007B03Rik/2310007B03Rik Standard Deviation 0.14 14.92 26.99 27.98 21.76 19.66 12.69
2310007B03Rik/2310007B03Rik Standard Error 0.04 4.14 7.49 7.76 6.04 5.45 3.52
Controls
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MAYP20.2d M00528633 +/+(locked) Female 13.8 25 30 20 25 30 30
MAYP20.2e M00528634 +/+(locked) Female 13.8 25 35 20 25 30 35
MBPH10.2g M00438457 +/+(locked) Female 14.2 20 30 10 20 30 35
MBPH10.2h M00438458 +/+(locked) Female 14.2 25 40 15 20 30 55
MBPH10.2i M00438459 +/+(locked) Female 14.2 25 30 15 15 30 45
MYFZ13.2d M00465327 +/+(locked) Female 14.1 25 35 15 25 35 50
MYFZ13.2e M00465328 +/+(locked) Female 14.1 20 40 20 20 30 40
MBFJ13.2a M00532217 +/+(locked) Male 13.9 20 25 15 10 20 35
MBFJ13.2c M00532219 +/+(locked) Male 13.9 25 30 15 20 20 30
MCBX12.2b M00438471 +/+(locked) Male 14.1 20 25 15 20 25 30
MCJP25.3b M00612201 +/+(locked) Male 14.2 25 25 10 10 20 25
MCJP25.3c M00612202 +/+(locked) Male 14.2 25 30 20 15 25 30
MCPL17.1e M00566255 +/+(locked) Male 14.1 25 30 10 20 25 35
MCPL21.1a M00566238 +/+(locked) Male 14.1 20 30 20 20 25 35
MCPL21.1c M00566240 +/+(locked) Male 14.1 20 30 15 15 25 30
+/+ Average 14.07 23 31 15.67 18.67 26.67 36
+/+ Standard Deviation 0.14 2.45 4.55 3.59 4.64 4.35 8
+/+ Standard Error 0.04 .63 1.17 .93 1.2 1.12 2.07