Adult LacZ Expression Data for Dusp3 (MBVF)

Overview

Adult expression (≥ 6 weeks): The lacZ reporter gene within the targeting vector was used to determine the wholemount expression profile of the targeted gene. Mice were fixed by cardiac perfusion using 4% paraformaldehyde (PFA). Following dissection, the tissues to be stained were fixed an additional 30 min in 4% PFA before being rinsed in phosphate buffered saline and transferred to lacZ staining solution, containing 0.1% X-gal, for up to 48 hours. After an additional overnight post-staining fixation in 4% PFA, tissues were cleared with 50% glycerol then transferred to 70% glycerol for long-term storage.

Display Criteria

A tick-list of 41 tissues and organs was scored for the presence (green tick) or absence (red cross) of lacZ expression.

  • The term 'Ambiguous' was assigned when there was uncertainty as to the validity of the observed pattern e.g. staining may be very faint, background or an artefact due to trapping.
  • The term 'No Data' was assigned if the stained entity was not available to assess.

By default, up to 27 standard images may be taken using a Leica MZ16A microscope and Imagic software. These images were only collected when X-gal staining was present.

If information or images that you are interested in are not presented, please send details to MGPenquiries@sanger.ac.uk and, on a limited, first come first served basis, we will process your request.

Tick-List
Colony Name Dusp3 Knockout 2 EPD0090_4_H11 Dusp3 Knockout 2 EPD0090_4_H11 Dusp3 Knockout 2 EPD0090_4_H11 Dusp3 Knockout 2 EPD0090_4_H11
Genotype Dusp3/+ Dusp3/+ Dusp3/+ Dusp3/+
Gender Male Female Male Female
Age In Weeks 7.5 7.5 12.2 12.2
Adrenal Gland absent absent absent absent
Bone ambiguous ambiguous absent absent
Brain present present present present
Brown Adipose Tissue absent absent absent absent
Cartilage absent absent absent absent
Colon present present absent absent
Eye absent absent absent absent
Gall Bladder No data No data absent absent
Heart present present present present
Kidney present present present present
Large Intestine present present absent absent
Liver absent absent absent absent
Lung present present absent absent
Mammary Gland ambiguous ambiguous absent absent
Mesenteric Lymph Node present present present present
Nasal Epithelia ambiguous ambiguous No data No data
Oesophagus absent absent absent absent
Oral Epithelia absent absent absent absent
Ovaries No data absent No data ambiguous
Oviduct No data absent No data absent
Pancreas ambiguous present absent absent
Parathyroid No data No data ambiguous ambiguous
Peripheral Nervous System absent absent absent absent
Peyers Patch absent absent absent absent
Pituitary Gland No data No data ambiguous No data
Prostate present No data absent No data
Skeletal Muscle absent absent absent absent
Skin absent absent absent absent
Small Intestine present present present absent
Spinal Cord present present present present
Spleen present present absent absent
Stomach present present present present
Testis present No data present No data
Thymus absent absent absent absent
Thyroid No data No data absent ambiguous
Trachea absent ambiguous absent absent
Urinary System ambiguous absent absent absent
Uterus No data absent No data absent
Vas Deferens present No data absent No data
Vascular System present present present present
White Adipose Tissue absent absent absent absent
Images
  • Tissue: Brain
    Description: Medial view
    Genotype: Heterozygous
  • Tissue: Brain
    Description: Lateral view
    Genotype: Heterozygous
  • Tissue: Heart
    Description: -
    Genotype: Heterozygous
  • Tissue: Hind limb
    Description: -
    Genotype: Heterozygous
  • Tissue: Lung
    Description: -
    Genotype: Heterozygous
  • Tissue: Rib cage
    Description: -
    Genotype: Heterozygous
  • Tissue: Skull
    Description: Internal view
    Genotype: Heterozygous
  • Tissue: Spleen
    Description: -
    Genotype: Heterozygous
  • Tissue: Stomach
    Description: -
    Genotype: Heterozygous
  • Tissue: Tail
    Description: Cross-section
    Genotype: Heterozygous
  • Tissue: Testis
    Description: -
    Genotype: Heterozygous
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Heterozygous
Background LacZ Staining in Adult

The adult wholemount LacZ expression protocol has been refined to minimize background staining however, it can still occur. We have systematically documented the sites and intensities of this background stain in male and female wildtype C57BL/6 (C57BL/6J-Tyr<c-Brd>; C57BL/6NTac/Den) mice. The major locations where background staining may be observed are:

  • Nasal cavity
  • Thyroid gland
  • Glandular stomach
  • Kidney
  • Male genitals

Additional background staining is observed occasionally in:

  • Rib cage
  • Thymus
  • Mesenteric lymph nodes
  • Ovaries

Examples of the pattern and level of background stain are presented below.

  • Tissue: Nasal cavity
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Nasal cavity
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Kidney
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Kidney
    Description: -
    Genotype: Wildtype
  • Tissue: Urogenital system
    Description: -
    Genotype: Wildtype
  • Tissue: Testis
    Description: -
    Genotype: Wildtype
  • Tissue: Rib cage
    Description: -
    Genotype: Wildtype
  • Tissue: Thymus
    Description: -
    Genotype: Wildtype
  • Tissue: Mesenteric lymph node
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype