Plasma Chemistry Data for Cdh23 (MBTS)
Fasted Plasma Chemistry (Ketamine / Xylazine) - significant parameters
Fasted Plasma Chemistry Metabolic Panel (week 15) [Mice were fed on High Fat Diet (21.4% crude fat content, Western RD, 829100, Special Diets Services) from 4 weeks of age]: Mice are fasted overnight (maximum 16 hours) then terminally anaesthetised with ketamine/xylazine, after which blood is collected into Lithium/Heparin coated tubes via the retro-orbital sinus. The resulting plasma is analysed for glucose, triglycerides, cholesterol, high density lipoproteins, low density lipoproteins, non-esterified free fatty acids (NEFAC) and glycerol on an Olympus AU400. For a subset of genes, fructosamine is analysed using the AU400 and plasma insulin concentration is measured by ELISA and/or Mesoscale Discovery (MSD) array technology.
view legend
| Legend - hover over each heading to get a more complete description | ||||||||
| Test complete and data/resources are available | Test is complete and the data are considered interesting | Preliminary indication of an interesting phenotype | ||||||
| Test is complete but the data are not considered interesting | Test not performed or applicable | Test pending | ||||||
| Test abandoned | ||||||||
| Parameter | Female Het |
Female Hom |
Male Het |
Male Hom |
population parameter |
Graph | MP Annotation |
|---|---|---|---|---|---|---|---|
| Glucose | view graph | MP:0005559 - increased circulating glucose level | |||||
| Triglycerides | |||||||
| Cholesterol | view graph | MP:0005179 - decreased circulating cholesterol level | |||||
| High Density Lipoprotein | view graph | MP:0000186 - decreased circulating HDL cholesterol level | |||||
| Low Density Lipoprotein | |||||||
| NEFAC | view graph | MP:0002702 - decreased circulating free fatty acid level | |||||
| Glycerol | view graph | MP:0003442 - decreased circulating glycerol level |
Plasma Chemistry (Ketamine / Xylazine) - significant parameters
Plasma Chemistry panel (week 14) (Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning): Non-fasted mice are terminally anaesthetised with ketamine/xylazine and blood is collected into Lithium/Heparin coated tubes via the retro-orbital sinus. Plasma is analysed for the following parameters on an Olympus AU400:
view legend
| Legend - hover over each heading to get a more complete description | ||||||||
| Test complete and data/resources are available | Test is complete and the data are considered interesting | Preliminary indication of an interesting phenotype | ||||||
| Test is complete but the data are not considered interesting | Test not performed or applicable | Test pending | ||||||
| Test abandoned | ||||||||
| Parameter | Female Het |
Female Hom |
Male Het |
Male Hom |
population parameter |
Graph | MP Annotation |
|---|---|---|---|---|---|---|---|
| Sodium | |||||||
| Potassium | |||||||
| Chloride | |||||||
| Glucose | |||||||
| Triglycerides | |||||||
| Cholesterol | view graph | MP:0005179 - decreased circulating cholesterol level | |||||
| High Density Lipoprotein | view graph | MP:0000186 - decreased circulating HDL cholesterol level | |||||
| Low Density Lipoprotein | view graph | MP:0000183 - decreased circulating LDL cholesterol level | |||||
| NEFAC | |||||||
| Glycerol | |||||||
| Amylase | view graph | MP:0008805 - decreased circulating amylase level | |||||
| Alanine Aminotransferase | |||||||
| Alkaline Phosphatase | |||||||
| Creatine Kinase | |||||||
| Aspartate Aminotransferase | |||||||
| Total Bilirubin | |||||||
| Total Protein | |||||||
| Albumin | |||||||
| Creatinine | |||||||
| Urea | |||||||
| Calcium | |||||||
| Magnesium | |||||||
| Iron | |||||||
| Phosphate | |||||||
| Lactate Dehydrogenase | |||||||
| Uric Acid |
