Phenotyping Data for Cdh23 (MBTS) Associated with 'homeostasis/metabolism' (MP:0005376)

Plasma Chemistry: Fasted Plasma Chemistry (Ketamine / Xylazine) - significant parameters
Fasted Plasma Chemistry Metabolic Panel (week 15) [Mice were fed on High Fat Diet (21.4% crude fat content, Western RD, 829100, Special Diets Services) from 4 weeks of age]: Mice are fasted overnight (maximum 16 hours) then terminally anaesthetised with ketamine/xylazine, after which blood is collected into Lithium/Heparin coated tubes via the retro-orbital sinus. The resulting plasma is analysed for glucose, triglycerides, cholesterol, high density lipoproteins, low density lipoproteins, non-esterified free fatty acids (NEFAC) and glycerol on an Olympus AU400. For a subset of genes, fructosamine is analysed using the AU400 and plasma insulin concentration is measured by ELISA and/or Mesoscale Discovery (MSD) array technology.
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Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Female
Hom
Male
Het
Male
Hom
population
parameter
Graph MP Annotation
Glucose view graph MP:0005559 - increased circulating glucose level
Triglycerides
Cholesterol view graph MP:0005179 - decreased circulating cholesterol level
High Density Lipoprotein view graph MP:0000186 - decreased circulating HDL cholesterol level
Low Density Lipoprotein
NEFAC view graph MP:0002702 - decreased circulating free fatty acid level
Glycerol view graph MP:0003442 - decreased circulating glycerol level
Plasma Chemistry: Plasma Chemistry (Ketamine / Xylazine) - significant parameters
Plasma Chemistry panel (week 14) (Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning): Non-fasted mice are terminally anaesthetised with ketamine/xylazine and blood is collected into Lithium/Heparin coated tubes via the retro-orbital sinus. Plasma is analysed for the following parameters on an Olympus AU400:
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Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Female
Hom
Male
Het
Male
Hom
population
parameter
Graph MP Annotation
Sodium
Potassium
Chloride
Glucose
Triglycerides
Cholesterol view graph MP:0005179 - decreased circulating cholesterol level
High Density Lipoprotein view graph MP:0000186 - decreased circulating HDL cholesterol level
Low Density Lipoprotein view graph MP:0000183 - decreased circulating LDL cholesterol level
NEFAC
Glycerol
Amylase view graph MP:0008805 - decreased circulating amylase level
Alanine Aminotransferase
Alkaline Phosphatase
Creatine Kinase
Aspartate Aminotransferase
Total Bilirubin
Total Protein
Albumin
Creatinine
Urea
Calcium
Magnesium
Iron
Phosphate
Lactate Dehydrogenase
Uric Acid
Indirect Calorimetry: Standard Protocol - significant parameters
Indirect Calorimetry (week 12, male mice prioritised) [Mice were fed on High Fat Diet (21.4% crude fat content, Western RD, 829100, Special Diets Services) from 4 weeks of age]: Respiratory metabolic function is assessed using the LabMaster system (TSE-systems). Mice are housed individually in calorimetry cages for a period of approximately 21 hours including a 5 hour light phase acclimatisation period. This is followed consecutively by a 12 hour dark (19:30 - 07:30) and minimum 3 hour light phase. Cumulative food intake, activity (beam breaks), volume of oxygen consumed and volume of carbon dioxide produced are all measured. From this data the respiratory exchange ratio and energy expenditure are derived. Water bottles are weighed before and after the experiment to assess water consumption.
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Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Female
Hom
Male
Het
Male
Hom
population
parameter
Graph MP Annotation
Respiratory Exchange Ratio view graph MP:0010378 - increased respiratory quotient
VCO2 view graph MP:0008963 - increased carbon dioxide production
VO2 view graph MP:0005289 - increased oxygen consumption
Energy Expenditure view graph MP:0004889 - increased energy expenditure
Energy Expenditure/Animal
Glucose Tolerance (ip): Standard Protocol - significant parameters
ipGTT (week 13) [Mice were fed on High Fat Diet (21.4% crude fat content, Western RD, 829100, Special Diets Services) from 4 weeks of age]: Mice are fasted overnight (maximum of 16 hours) before the glucose tolerance test. A fasting blood sample is taken before a bolus of glucose is administered by intra-peritoneal injection. Blood samples are tested for glucose concentration (Accu-Check Aviva, Roche) at 15, 30, 60 and 120 minutes following the glucose administration, and data presented as plasma glucose concentration.
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Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Female
Hom
Male
Het
Male
Hom
population
parameter
Graph MP Annotation
ip-GTT view graph MP:0005292 - improved glucose tolerance
Fasted Plasma Glucose
Stress Induced Hyperthermia: Core Temperature
Core Temperature (week 14) [Mice were fed on High Fat Diet (21.4% crude fat content, Western RD, 829100, Special Diets Services) from 4 weeks of age]: Rectal core body temperature is measured using a TH-5 thermometer with a RET-3 probe (Viking Medical).
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Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Female
Hom
Male
Het
Male
Hom
population
parameter
Graph MP Annotation
Core Temperature