Phenotyping Data for Cdh23 (MBTS) Associated with 'behavior/neurological' (MP:0005386)

Modified SHIRPA: Modified SHIRPA v3 - significant parameters
Modified SHIRPA (week 9) (Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning): Mice are assessed for gross behavioural abnormalities using a modified SHIRPA screen.
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Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Hom
Male
Hom
population
parameter
Graph MP Annotation
Body Position view graph MP:0001399 - hyperactivity
Gait (inc. Ataxia) view graph MP:0001408 - stereotypic behavior
Tail Elevation view graph MP:0005507 - tail dragging
Startle Response view graph MP:0006358 - absent pinna reflex
Trunk Curl view graph MP:0001512 - trunk curl
Limb Grasping view graph MP:0001513 - limb grasping
Pinna touch reflex view graph MP:0001491 - unresponsive to tactile stimuli
Contact Righting Reflex view graph MP:0001523 - impaired righting response
Open Field: Open Field (20 minutes) - significant parameters
Open Field (week 9) (Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning): Anxiety and exploratory drive are assessed over 20 minutes using open field apparatus (PhenoMaster ActiMot; TSE Systems)
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Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Female
Hom
Male
Het
Male
Hom
population
parameter
Graph MP Annotation
Total Distance Travelled Whole Arena
Whole Arena Resting Time
Whole Arena Average Speed
Total Rears view graph MP:0002574 - increased vertical activity
Total Distance Travelled - Periphery
Total Rest Time at Periphery
Total Time at Periphery
Average Speed at Periphery
Total Distance Travelled - Centre
Total Resting Time in Centre
Total Time in Centre
Average Speed at Centre
Latency To Enter Centre
Number Entries to Centre
% Distance Moved in Centre - Total view graph MP:0004924 - abnormal behavior
% Time Spent in Centre - Total
Hot Plate: Standard Protocol
Hotplate (week 12) (Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning): Mice are assessed for thermal pain perception using a hotplate (TSE systems). Each mouse is placed on the plate heated to 52?C, and the latency to the first response, and response type, are recorded. Mice that do not respond after 30 seconds are removed.
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Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Female
Hom
Male
Het
Male
Hom
population
parameter
Graph MP Annotation
Latency to respond
Eye Morphology: Standard Protocol
Eye Morphology (week 13) (Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning): Mice are assessed for gross morphological changes to the eye using a slit lamp (Zeiss SL130) and ophthalmoscope (Heine Omega 500). The eye is examined both undilated and dilated (tropicamide and/or neosynephrine). Images on the slit lamp are collected using a Leica DFC420. Images of the fundus are collected by using a topical endoscope (BERCI Tele-Otoscope with HOPKINS straight forward 0?, diameter 3mm, Halogen cold light fountain light source) and camera (Nikon D40x with Nikon AF 85mm F1.8D AF Nikkor lens).
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Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Female
Hom
Male
Het
Male
Hom
population
parameter
Graph MP Annotation
Pupil Light Response
Rotarod: Standard Protocol - significant parameters
Rotarod (week 10) (Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning): Mice are assessed for motor activity and coordination by rotarod (Bioseb). Three 5 minute trials on a rod constantly accelerating from 4-40rpm are performed with a 15 minute inter-trial interval, and the latency and reason for ending each trial is recorded. The trial is ended when the mouse either falls off the rod, jumps off the rod, or performs a passive rotation (the mouse grasps the surface of the rod and rides around the rod instead of walking on it).
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Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Hom
Male
Hom
population
parameter
Graph MP Annotation
Latency to Fall Trial 1 view graph MP:0001405 - impaired coordination
Latency to Fall Trial 2 view graph MP:0001405 - impaired coordination
Latency to Fall Trial 3 view graph MP:0001405 - impaired coordination
Grip Strength: Standard Protocol - significant parameters
Grip Strength (week 9) (Mice were fed on Mouse Breeder Diet (5021, Labdiet) from weaning): Neuromuscular function and muscle strength are assessed with a Bioseb grip strength meter. Three trials each of fore paws and all paws are performed immediately following each other.
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Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Female
Hom
Male
Het
Male
Hom
population
parameter
Graph MP Annotation
Grip Strength Fore Paws Trial 1 view graph MP:0010053 - decreased grip strength
Grip Strength Fore Paws Trial 2 view graph MP:0010053 - decreased grip strength
Grip Strength Fore Paws Trial 3 view graph MP:0010053 - decreased grip strength
Grip Strength All Paws Trial 1 view graph MP:0010053 - decreased grip strength
Grip Strength All Paws Trial 2 view graph MP:0010053 - decreased grip strength
Grip Strength All Paws Trial 3 view graph MP:0010053 - decreased grip strength
Indirect Calorimetry: Standard Protocol - significant parameters
Indirect Calorimetry (week 12, male mice prioritised) [Mice were fed on High Fat Diet (21.4% crude fat content, Western RD, 829100, Special Diets Services) from 4 weeks of age]: Respiratory metabolic function is assessed using the LabMaster system (TSE-systems). Mice are housed individually in calorimetry cages for a period of approximately 21 hours including a 5 hour light phase acclimatisation period. This is followed consecutively by a 12 hour dark (19:30 - 07:30) and minimum 3 hour light phase. Cumulative food intake, activity (beam breaks), volume of oxygen consumed and volume of carbon dioxide produced are all measured. From this data the respiratory exchange ratio and energy expenditure are derived. Water bottles are weighed before and after the experiment to assess water consumption.
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Legend - hover over each heading to get a more complete description
Test complete and data/resources are available Test is complete and the data are considered interesting Preliminary indication of an interesting phenotype
Test is complete but the data are not considered interesting Test not performed or applicable Test pending
Test abandoned
Parameter Female
Het
Female
Hom
Male
Het
Male
Hom
population
parameter
Graph MP Annotation
Cumulative Food Intake view graph MP:0003909 - increased eating behavior
Water Intake
X-Activity Total view graph MP:0001399 - hyperactivity
X-Activity Ambulatory view graph MP:0001399 - hyperactivity