Adult LacZ Expression Data for Ltbp1 (MBSE)

Overview

Adult expression (≥ 6 weeks): The lacZ reporter gene within the targeting vector was used to determine the wholemount expression profile of the targeted gene. Mice were fixed by cardiac perfusion using 4% paraformaldehyde (PFA). Following dissection, the tissues to be stained were fixed an additional 30 min in 4% PFA before being rinsed in phosphate buffered saline and transferred to lacZ staining solution, containing 0.1% X-gal, for up to 48 hours. After an additional overnight post-staining fixation in 4% PFA, tissues were cleared with 50% glycerol then transferred to 70% glycerol for long-term storage.

Display Criteria

A tick-list of 41 tissues and organs was scored for the presence (green tick) or absence (red cross) of lacZ expression.

  • The term 'Ambiguous' was assigned when there was uncertainty as to the validity of the observed pattern e.g. staining may be very faint, background or an artefact due to trapping.
  • The term 'No Data' was assigned if the stained entity was not available to assess.

By default, up to 27 standard images may be taken using a Leica MZ16A microscope and Imagic software. These images were only collected when X-gal staining was present.

If information or images that you are interested in are not presented, please send details to MGPenquiries@sanger.ac.uk and, on a limited, first come first served basis, we will process your request.

Tick-List
Colony Name Ltbp1 Knockout First EPD0127_1_A03(1) Ltbp1 Knockout First EPD0127_1_A03(1) Ltbp1 Knockout First EPD0127_1_A03(1)
Genotype Ltbp1/+ Ltbp1/+ Ltbp1/+
Gender Male Female Female
Age In Weeks 10.1 6.6 8.9
Adrenal Gland absent absent No data
Bone absent absent absent
Brain absent absent absent
Brown Adipose Tissue absent absent absent
Cartilage absent absent absent
Colon absent absent ambiguous
Eye absent absent absent
Gall Bladder present absent present
Heart present ambiguous present
Kidney ambiguous ambiguous present
Large Intestine absent absent ambiguous
Liver absent present absent
Lung absent absent present
Mammary Gland absent absent absent
Mesenteric Lymph Node absent absent No data
Nasal Epithelia No data No data No data
Oesophagus absent absent absent
Oral Epithelia absent absent absent
Ovaries No data present present
Oviduct No data present present
Pancreas absent absent absent
Parathyroid absent absent absent
Peripheral Nervous System absent absent absent
Peyers Patch absent absent absent
Pituitary Gland absent absent absent
Prostate absent No data No data
Skeletal Muscle absent absent absent
Skin absent absent absent
Small Intestine ambiguous absent present
Spinal Cord absent absent absent
Spleen absent absent absent
Stomach present present present
Testis present No data No data
Thymus absent absent absent
Thyroid absent ambiguous present
Trachea present absent present
Urinary System present ambiguous present
Uterus No data absent present
Vas Deferens absent No data No data
Vascular System present present present
White Adipose Tissue absent absent absent
Images
  • Tissue: Bladder
    Description: -
    Genotype: Heterozygous
  • Tissue: Brain
    Description: -
    Genotype: Heterozygous
  • Tissue: Brain
    Description: Lateral view
    Genotype: Heterozygous
  • Tissue: Heart
    Description: -
    Genotype: Heterozygous
  • Tissue: Hind limb
    Description: -
    Genotype: Heterozygous
  • Tissue: Liver and gall bladder
    Description: -
    Genotype: Heterozygous
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Heterozygous
  • Tissue: Small intestine
    Description: -
    Genotype: Heterozygous
  • Tissue: Stomach
    Description: -
    Genotype: Heterozygous
  • Tissue: Tail
    Description: Cross-section
    Genotype: Heterozygous
  • Tissue: Testis
    Description: -
    Genotype: Heterozygous
  • Tissue: Trachea and thyroid
    Description: Dorsal view
    Genotype: Heterozygous
Background LacZ Staining in Adult

The adult wholemount LacZ expression protocol has been refined to minimize background staining however, it can still occur. We have systematically documented the sites and intensities of this background stain in male and female wildtype C57BL/6 (C57BL/6J-Tyr<c-Brd>; C57BL/6NTac/Den) mice. The major locations where background staining may be observed are:

  • Nasal cavity
  • Thyroid gland
  • Glandular stomach
  • Kidney
  • Male genitals

Additional background staining is observed occasionally in:

  • Rib cage
  • Thymus
  • Mesenteric lymph nodes
  • Ovaries

Examples of the pattern and level of background stain are presented below.

  • Tissue: Nasal cavity
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Nasal cavity
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Kidney
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Kidney
    Description: -
    Genotype: Wildtype
  • Tissue: Urogenital system
    Description: -
    Genotype: Wildtype
  • Tissue: Testis
    Description: -
    Genotype: Wildtype
  • Tissue: Rib cage
    Description: -
    Genotype: Wildtype
  • Tissue: Thymus
    Description: -
    Genotype: Wildtype
  • Tissue: Mesenteric lymph node
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype