Auditory Brainstem Response (ABR) measurement for Amfrtm1a(KOMP)WtsiMouse GP

Results Summary

  • ABR thresholds normal
  • Click-ABR waveforms normal

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean threshold is plotted (+/- population standard deviation). The green shaded area indicates the median threshold (and 2.5% to 97.5% reference range) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Superimposed waveforms recorded at 20dB and 50dB sensation level were plotted. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and were plotted as a function of dB sensation level. Means are plotted (over a 95% reference range for each parameter).

ABR Thresholds Summary

ABR Thresholds for Amfr<tm1a(KOMP)Wtsi> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MBPL51.2j M00438065 Amfr/+(locked) Female 14.2 20 30 10 15 25 30
MBPL36.1a M00303370 Amfr/+(locked) Male 14.2 25 35 20 25 25 30
MBPL36.1b M00303371 Amfr/+(locked) Male 14.2 25 30 10 25 35 35
MBPL37.1a M00306777 Amfr/+(locked) Male 13.9 25 35 15 20 30 35
MBPL37.1b M00306778 Amfr/+(locked) Male 13.9 25 35 15 20 30 40
Amfr/+ Average 14.08 24 33 14 21 29 34
Amfr/+ Standard Deviation 0.15 2 2.45 3.74 3.74 3.74 3.74
Amfr/+ Standard Error 0.07 .89 1.1 1.67 1.67 1.67 1.67
MBPL51.2i M00438064 Amfr/Amfr(locked) Female 14.2 25 30 20 20 30 45
MBPL51.2k M00438066 Amfr/Amfr(locked) Female 14.2 20 35 15 15 30 35
MBPL51.2l M00438067 Amfr/Amfr(locked) Female 14.2 20 30 10 20 25 30
MBPL54.1e M00456900 Amfr/Amfr(locked) Female 13.9 25 25 15 15 25 35
MBPL54.1f M00457362 Amfr/Amfr(locked) Female 13.9 20 25 15 15 30 40
MBPL54.1g M00457363 Amfr/Amfr(locked) Female 13.9 20 35 15 15 35 40
MBPL51.1b M00405968 Amfr/Amfr(locked) Male 14.1 20 25 10 15 20 25
MBPL51.1d M00405970 Amfr/Amfr(locked) Male 14.1 25 30 10 20 35 30
MBPL51.1f M00405972 Amfr/Amfr(locked) Male 14.1 25 35 15 20 25 35
MBPL54.1a M00456896 Amfr/Amfr(locked) Male 13.9 20 30 15 20 30 30
Amfr/Amfr Average 14.05 22 30 14 17.5 28.5 34.5
Amfr/Amfr Standard Deviation 0.13 2.45 3.87 3 2.5 4.5 5.68
Amfr/Amfr Standard Error 0.04 .77 1.22 .95 .79 1.42 1.8
Controls
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MALR24.3c M00445260 +/+(locked) Female 13.8 20 35 15 25 25 25
MALR24.3d M00445261 +/+(locked) Female 13.8 25 30 15 15 25 30
MALR30.3e M00458248 +/+(locked) Female 14.1 25 30 15 15 20 35
MALR30.3f M00458249 +/+(locked) Female 14.1 25 30 5 25 30 40
MBDZ52.1d M00318816 +/+(locked) Female 14.1 25 40 15 25 30 35
MBDZ52.1e M00318817 +/+(locked) Female 14.1 25 35 20 20 30 30
MBFD63.2e M00338901 +/+(locked) Female 13.9 20 30 5 10 20 20
MBFD66.3b M00338947 +/+(locked) Female 13.8 20 30 15 10 25 30
MBPZ45.1f M00311511 +/+(locked) Female 13.9 25 30 10 20 25 35
MBPZ45.1i M00311514 +/+(locked) Female 13.9 25 35 15 25 30 35
MAHU28.2a M00316942 +/+(locked) Male 14.3 25 40 15 20 30 40
MAHU48.2a M00323682 +/+(locked) Male 14.3 25 30 15 15 25 40
MAKT55.2a M00405977 +/+(locked) Male 14.2 20 35 15 25 20 30
MAKT55.2c M00405979 +/+(locked) Male 14.2 10 35 10 20 25 30
MBLT24.1a M00302765 +/+(locked) Male 14.5 25 40 25 25 35 35
MBLT24.1b M00302766 +/+(locked) Male 14.5 25 40 25 25 30 30
MBRS49.4a M00454480 +/+(locked) Male 14.1 20 35 20 20 25 40
MBRS49.4b M00454481 +/+(locked) Male 14.1 20 30 10 15 20 30
MCFC17.1b M00340778 +/+(locked) Male 13.9 20 30 5 10 20 30
MCFC17.1d M00340780 +/+(locked) Male 13.9 20 35 15 15 25 35
+/+ Average 14.08 22.25 33.75 14.25 19 25.75 32.75
+/+ Standard Deviation 0.21 3.7 3.83 5.54 5.39 4.26 5.12
+/+ Standard Error 0.05 .83 .86 1.24 1.2 .95 1.14