Auditory Brainstem Response (ABR) measurement for Spns2tm1a(KOMP)WtsiMouse GP

Results Summary

  • ABR thresholds elevated across all frequencies tested
  • Severe Impairment

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean threshold is plotted (+/- population standard deviation). The green shaded area indicates the median threshold (and 2.5% to 97.5% reference range) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Superimposed waveforms recorded at 20dB and 50dB sensation level were plotted. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and were plotted as a function of dB sensation level. Means are plotted (over a 95% reference range for each parameter).

ABR Thresholds Summary

ABR Thresholds for Spns2<tm1a(KOMP)Wtsi> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MBNZ55.3b M00351774 Spns2/Spns2(locked) Female 13.6 90 95 95 95 95 95
MBNZ55.3c M00351775 Spns2/Spns2(locked) Female 13.6 95 85 80 95 95 95
MBNZ16.1a M00180555 Spns2/Spns2(locked) Male 14.1 95 95 95 90 95 95
MBNZ16.1b M00180556 Spns2/Spns2(locked) Male 14.1 95 95 95 95 95 95
MBNZ21.1a M00180614 Spns2/Spns2(locked) Male 13.9 80 80 80 95 95 95
MBNZ21.2c M00194278 Spns2/Spns2(locked) Male 13.8 95 95 95 95 95 95
Spns2/Spns2 Average 13.85 91.67 90.83 90 94.17 95 95
Spns2/Spns2 Standard Deviation 0.21 5.53 6.07 7.07 1.86 0 0
Spns2/Spns2 Standard Error 0.08 2.26 2.48 2.89 .76 0 0
MBNZ21.1e M00180618 Spns2:failed(locked) Female 13.9 95 90 85 95 95 95
MBNZ18.2b M00188213 Spns2:failed(locked) Male 14.1 20 45 15 20 20 30
Spns2:failed Average 14.0 57.5 67.5 50 57.5 57.5 62.5
Spns2:failed Standard Deviation 0.1 37.5 22.5 35 37.5 37.5 32.5
Spns2:failed Standard Error 0.07 26.52 15.91 24.75 26.52 26.52 22.98
Controls
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MBCY145.3d M00532528 +/+(locked) Female 14.2 25 35 10 20 25 35
MBCY145.3e M00532529 +/+(locked) Female 14.2 25 35 15 20 25 30
MBDZ51.2e M00351274 +/+(locked) Female 13.6 20 25 10 15 25 25
MBDZ51.2f M00351275 +/+(locked) Female 13.6 20 25 10 15 20 25
MBGX97.1c M00375559 +/+(locked) Female 14.1 20 35 15 15 25 30
MBGX97.1f M00375562 +/+(locked) Female 14.1 25 35 15 20 25 40
MBHE14.1d M00180648 +/+(locked) Female 14.1 20 35 10 15 25 40
MBNY15.1g M00177967 +/+(locked) Female 13.9 25 35 15 10 40 45
MBNY15.1i M00177969 +/+(locked) Female 13.9 25 30 25 20 35 35
MBNY22.1h M00178068 +/+(locked) Female 14.1 20 35 15 15 30 40
MBNY23.1h M00178076 +/+(locked) Female 14.1 25 35 20 15 35 30
MBNZ16.1f M00180560 +/+(locked) Female 14.1 20 25 15 15 30 30
MAKR88.1a M00369124 +/+(locked) Male 14.3 25 35 10 15 30 40
MAKR89.1b M00369127 +/+(locked) Male 14.5 30 35 20 25 30 40
MALA17.2b M00194356 +/+(locked) Male 14.4 20 30 10 10 20 35
MANH12.2e M00190572 +/+(locked) Male 14.3 20 30 15 25 25 35
MANH12.2f M00190573 +/+(locked) Male 14.3 20 35 20 10 30 35
MAOA10.2d M00191891 +/+(locked) Male 14.2 25 40 15 30 30 35
MAOA12.1a M00191899 +/+(locked) Male 14.1 20 40 15 30 25 35
MBNZ18.1a M00176614 +/+(locked) Male 14.2 20 30 10 15 20 30
MBNZ21.2a M00194276 +/+(locked) Male 13.8 20 30 15 15 20 30
MBNZ22.1b M00180633 +/+(locked) Male 13.8 25 30 15 15 25 30
MBNZ22.1c M00180634 +/+(locked) Male 13.8 20 20 10 10 20 30
+/+ Average 14.07 22.39 32.17 14.35 17.17 26.74 33.91
+/+ Standard Deviation 0.23 2.9 4.85 3.98 5.68 5.24 5.1
+/+ Standard Error 0.05 .6 1.01 .83 1.18 1.09 1.06