Adult LacZ Expression Data for Spns2 (MBNZ)

Overview

Adult expression (≥ 6 weeks): The lacZ reporter gene within the targeting vector was used to determine the wholemount expression profile of the targeted gene. Mice were fixed by cardiac perfusion using 4% paraformaldehyde (PFA). Following dissection, the tissues to be stained were fixed an additional 30 min in 4% PFA before being rinsed in phosphate buffered saline and transferred to lacZ staining solution, containing 0.1% X-gal, for up to 48 hours. After an additional overnight post-staining fixation in 4% PFA, tissues were cleared with 50% glycerol then transferred to 70% glycerol for long-term storage.

Display Criteria

A tick-list of 41 tissues and organs was scored for the presence (green tick) or absence (red cross) of lacZ expression.

  • The term 'Ambiguous' was assigned when there was uncertainty as to the validity of the observed pattern e.g. staining may be very faint, background or an artefact due to trapping.
  • The term 'No Data' was assigned if the stained entity was not available to assess.

By default, up to 27 standard images may be taken using a Leica MZ16A microscope and Imagic software. These images were only collected when X-gal staining was present.

If information or images that you are interested in are not presented, please send details to MGPenquiries@sanger.ac.uk and, on a limited, first come first served basis, we will process your request.

Tick-List
Colony Name Spns2 Knockout First EPD0090_5_B04 Spns2 Knockout First EPD0090_5_B04 Spns2 Knockout First EPD0090_5_B04 Spns2 Knockout First EPD0090_5_B04 Spns2 Knockout First EPD0090_5_B04
Genotype Spns2/+ Spns2/+ Spns2/+ Spns2/+ Spns2/+
Gender Male Female Male Male Female
Age In Weeks 8.5 8.5 10.4 6.8 6.8
Adrenal Gland absent absent absent absent absent
Bone present present present present present
Brain present present present present present
Brown Adipose Tissue absent absent absent absent absent
Cartilage absent absent absent absent absent
Colon absent absent absent absent absent
Eye present absent present present absent
Gall Bladder No data No data absent absent absent
Heart absent absent absent absent absent
Kidney present present present present present
Large Intestine absent absent absent absent absent
Liver present present present present present
Lung absent absent absent absent absent
Mammary Gland absent absent absent absent absent
Mesenteric Lymph Node present present present present present
Nasal Epithelia absent absent No data No data No data
Oesophagus present present present present present
Oral Epithelia present present present present present
Ovaries No data absent No data No data absent
Oviduct No data present No data No data ambiguous
Pancreas absent absent absent absent absent
Parathyroid No data No data absent absent absent
Peripheral Nervous System absent absent ambiguous absent absent
Peyers Patch present present present absent absent
Pituitary Gland No data No data absent absent absent
Prostate absent No data absent absent No data
Skeletal Muscle absent absent absent absent absent
Skin present present present present present
Small Intestine absent absent absent absent absent
Spinal Cord present present present present present
Spleen absent absent present absent absent
Stomach present present present present present
Testis present No data present present No data
Thymus absent absent absent absent absent
Thyroid No data No data ambiguous absent absent
Trachea absent absent absent absent absent
Urinary System present present absent present present
Uterus No data No data No data No data absent
Vas Deferens present No data absent absent No data
Vascular System absent absent absent absent absent
White Adipose Tissue absent absent absent absent absent
Images
  • Tissue: Bladder
    Description: -
    Genotype: Heterozygous
  • Tissue: Brain
    Description: Lateral view
    Genotype: Heterozygous
  • Tissue: Brain
    Description: Longitudinal section
    Genotype: Heterozygous
  • Tissue: Eye
    Description: -
    Genotype: Heterozygous
  • Tissue: Inguinal fat pad
    Description: -
    Genotype: Heterozygous
  • Tissue: Kidney
    Description: Longitudinal section
    Genotype: Heterozygous
  • Tissue: Knee joint
    Description: -
    Genotype: Heterozygous
  • Tissue: Liver and gall bladder
    Description: -
    Genotype: Heterozygous
  • Tissue: Pinna
    Description: -
    Genotype: Heterozygous
  • Tissue: Skin
    Description: -
    Genotype: Heterozygous
  • Tissue: Stomach
    Description: -
    Genotype: Heterozygous
  • Tissue: Tail
    Description: Cross-section
    Genotype: Heterozygous
  • Tissue: Testis
    Description: -
    Genotype: Heterozygous
  • Tissue: Testis
    Description: -
    Genotype: Heterozygous
  • Tissue: Urogenital system
    Description: -
    Genotype: Heterozygous
Background LacZ Staining in Adult

The adult wholemount LacZ expression protocol has been refined to minimize background staining however, it can still occur. We have systematically documented the sites and intensities of this background stain in male and female wildtype C57BL/6 (C57BL/6J-Tyr<c-Brd>; C57BL/6NTac/Den) mice. The major locations where background staining may be observed are:

  • Nasal cavity
  • Thyroid gland
  • Glandular stomach
  • Kidney
  • Male genitals

Additional background staining is observed occasionally in:

  • Rib cage
  • Thymus
  • Mesenteric lymph nodes
  • Ovaries

Examples of the pattern and level of background stain are presented below.

  • Tissue: Nasal cavity
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Nasal cavity
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Kidney
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Kidney
    Description: -
    Genotype: Wildtype
  • Tissue: Urogenital system
    Description: -
    Genotype: Wildtype
  • Tissue: Testis
    Description: -
    Genotype: Wildtype
  • Tissue: Rib cage
    Description: -
    Genotype: Wildtype
  • Tissue: Thymus
    Description: -
    Genotype: Wildtype
  • Tissue: Mesenteric lymph node
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype