Auditory Brainstem Response (ABR) measurement for Fam107btm1a(KOMP)WtsiMouse GP

Results Summary

  • ABR thresholds elevated at mid-range frequencies (12-18kHz)
  • Mild Impairment
  • Click ABR waveform shape abnormal

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean threshold is plotted (+/- population standard deviation). The green shaded area indicates the median threshold (and 2.5% to 97.5% reference range) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Superimposed waveforms recorded at 20dB and 50dB sensation level were plotted. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and were plotted as a function of dB sensation level. Means are plotted (over a 95% reference range for each parameter).

ABR Thresholds Summary

ABR Thresholds for Fam107b<tm1a(KOMP)Wtsi> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MBNT39.1e M00251300 3110001A13Rik/3110001A13Rik(locked) Female 14.4 45 40 45 35 40 40
MBNT39.1f M00251301 3110001A13Rik/3110001A13Rik(locked) Female 14.4 30 45 40 45 40 40
MBNT40.1g M00251313 3110001A13Rik/3110001A13Rik(locked) Female 14.4 30 40 20 30 35 45
MBNT40.1h M00251314 3110001A13Rik/3110001A13Rik(locked) Female 14.4 45 45 40 45 40 40
MBNT47.1a M00326303 3110001A13Rik/3110001A13Rik(locked) Male 13.9 35 40 30 25 45 55
MBNT47.1b M00326304 3110001A13Rik/3110001A13Rik(locked) Male 13.9 40 35 30 50 35 40
MBNT47.1c M00326305 3110001A13Rik/3110001A13Rik(locked) Male 13.9 40 30 25 35 50 45
MBNT47.1d M00326306 3110001A13Rik/3110001A13Rik(locked) Male 13.9 40 40 50 40 40 50
3110001A13Rik/3110001A13Rik Average 14.15 38.13 39.38 35 38.13 40.63 44.38
3110001A13Rik/3110001A13Rik Standard Deviation 0.25 5.56 4.64 9.68 7.88 4.64 5.27
3110001A13Rik/3110001A13Rik Standard Error 0.09 1.96 1.64 3.42 2.79 1.64 1.86
Controls
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MBCU33.2f M00257518 +/+(locked) Female 14.2 25 30 15 25 20 30
MBCU33.2g M00257519 +/+(locked) Female 14.2 20 35 20 15 30 30
MBCU39.2g M00257528 +/+(locked) Female 14.4 20 35 20 20 25 30
MBCU39.2h M00257529 +/+(locked) Female 14.4 20 30 15 20 30 45
MBEJ14.2h M00286604 +/+(locked) Female 13.9 25 35 10 10 30 65
MBEJ19.1c M00286585 +/+(locked) Female 13.9 25 35 15 20 30 45
MAHU28.2a M00316942 +/+(locked) Male 14.3 25 40 15 20 30 40
MAHU48.2a M00323682 +/+(locked) Male 14.3 25 30 15 15 25 40
MBSR21.1d M00285218 +/+(locked) Male 14.3 20 40 10 20 25 25
MBSR21.1e M00285219 +/+(locked) Male 14.3 20 35 10 20 25 25
+/+ Average 14.22 22.5 34.5 14.5 18.5 27 37.5
+/+ Standard Deviation 0.17 2.5 3.5 3.5 3.91 3.32 11.67
+/+ Standard Error 0.05 .79 1.11 1.11 1.23 1.05 3.69

Waveform Overlay 20dB SL

Waveform Overlay 50dB SL