Adult LacZ Expression Data for Mis18bp1 (MBLK)

Overview

Adult expression (≥ 6 weeks): The lacZ reporter gene within the targeting vector was used to determine the wholemount expression profile of the targeted gene. Mice were fixed by cardiac perfusion using 4% paraformaldehyde (PFA). Following dissection, the tissues to be stained were fixed an additional 30 min in 4% PFA before being rinsed in phosphate buffered saline and transferred to lacZ staining solution, containing 0.1% X-gal, for up to 48 hours. After an additional overnight post-staining fixation in 4% PFA, tissues were cleared with 50% glycerol then transferred to 70% glycerol for long-term storage.

Display Criteria

A tick-list of 41 tissues and organs was scored for the presence (green tick) or absence (red cross) of lacZ expression.

  • The term 'Ambiguous' was assigned when there was uncertainty as to the validity of the observed pattern e.g. staining may be very faint, background or an artefact due to trapping.
  • The term 'No Data' was assigned if the stained entity was not available to assess.

By default, up to 27 standard images may be taken using a Leica MZ16A microscope and Imagic software. These images were only collected when X-gal staining was present.

If information or images that you are interested in are not presented, please send details to MGPenquiries@sanger.ac.uk and, on a limited, first come first served basis, we will process your request.

Tick-List
Colony Name Mis18bp1 Knockout First EPD0103_2_B05 Mis18bp1 Knockout First EPD0103_2_B05
Genotype Mis18bp1/+ Mis18bp1/+
Gender Male Female
Age In Weeks 7.5 9.1
Adrenal Gland absent absent
Bone ambiguous absent
Brain absent absent
Brown Adipose Tissue absent absent
Cartilage ambiguous present
Colon present present
Eye absent absent
Gall Bladder No data No data
Heart absent absent
Kidney absent absent
Large Intestine absent absent
Liver absent absent
Lung absent absent
Mammary Gland absent absent
Mesenteric Lymph Node absent present
Nasal Epithelia No data No data
Oesophagus absent absent
Oral Epithelia absent absent
Ovaries No data present
Oviduct No data absent
Pancreas absent absent
Parathyroid No data No data
Peripheral Nervous System absent absent
Peyers Patch present present
Pituitary Gland No data No data
Prostate absent No data
Skeletal Muscle absent absent
Skin absent ambiguous
Small Intestine absent absent
Spinal Cord absent absent
Spleen absent absent
Stomach absent absent
Testis present No data
Thymus ambiguous present
Thyroid No data No data
Trachea absent absent
Urinary System absent absent
Uterus No data absent
Vas Deferens absent No data
Vascular System absent absent
White Adipose Tissue absent absent
Images
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Heterozygous
  • Tissue: Peyer's patch
    Description: -
    Genotype: Heterozygous
  • Tissue: Testis
    Description: -
    Genotype: Heterozygous
  • Tissue: Thymus
    Description: -
    Genotype: Heterozygous
Background LacZ Staining in Adult

The adult wholemount LacZ expression protocol has been refined to minimize background staining however, it can still occur. We have systematically documented the sites and intensities of this background stain in male and female wildtype C57BL/6 (C57BL/6J-Tyr<c-Brd>; C57BL/6NTac/Den) mice. The major locations where background staining may be observed are:

  • Nasal cavity
  • Thyroid gland
  • Glandular stomach
  • Kidney
  • Male genitals

Additional background staining is observed occasionally in:

  • Rib cage
  • Thymus
  • Mesenteric lymph nodes
  • Ovaries

Examples of the pattern and level of background stain are presented below.

  • Tissue: Nasal cavity
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Nasal cavity
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Kidney
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Kidney
    Description: -
    Genotype: Wildtype
  • Tissue: Urogenital system
    Description: -
    Genotype: Wildtype
  • Tissue: Testis
    Description: -
    Genotype: Wildtype
  • Tissue: Rib cage
    Description: -
    Genotype: Wildtype
  • Tissue: Thymus
    Description: -
    Genotype: Wildtype
  • Tissue: Mesenteric lymph node
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype