Adult LacZ Expression Data for Nsun2 (MBKW)

Overview

Adult expression (≥ 6 weeks): The lacZ reporter gene within the targeting vector was used to determine the wholemount expression profile of the targeted gene. Mice were fixed by cardiac perfusion using 4% paraformaldehyde (PFA). Following dissection, the tissues to be stained were fixed an additional 30 min in 4% PFA before being rinsed in phosphate buffered saline and transferred to lacZ staining solution, containing 0.1% X-gal, for up to 48 hours. After an additional overnight post-staining fixation in 4% PFA, tissues were cleared with 50% glycerol then transferred to 70% glycerol for long-term storage.

Display Criteria

A tick-list of 41 tissues and organs was scored for the presence (green tick) or absence (red cross) of lacZ expression.

  • The term 'Ambiguous' was assigned when there was uncertainty as to the validity of the observed pattern e.g. staining may be very faint, background or an artefact due to trapping.
  • The term 'No Data' was assigned if the stained entity was not available to assess.

By default, up to 27 standard images may be taken using a Leica MZ16A microscope and Imagic software. These images were only collected when X-gal staining was present.

If information or images that you are interested in are not presented, please send details to MGPenquiries@sanger.ac.uk and, on a limited, first come first served basis, we will process your request.

Tick-List
Colony Name Nsun2 Knockout First EPD0105_2_F10 Nsun2 Knockout First EPD0105_2_F10 Nsun2 Knockout First EPD0105_2_F10 Nsun2 Knockout First EPD0105_2_F10 Nsun2 Knockout First EPD0105_2_F10 Nsun2 Knockout First EPD0105_2_F10
Genotype Nsun2/+ Nsun2/+ Nsun2/+ Nsun2/+ Nsun2/+ Nsun2/+
Gender Male Female Male Male Female Female
Age In Weeks 11.8 11.8 7.7 7.6 7.7 7.7
Adrenal Gland absent present present present present present
Bone ambiguous present present present present present
Brain present present present present present present
Brown Adipose Tissue absent absent absent absent absent ambiguous
Cartilage absent present present present present present
Colon absent present present present present present
Eye absent present present present present present
Gall Bladder No data No data present present ambiguous ambiguous
Heart absent present present present present present
Kidney present present present present present present
Large Intestine absent present present present present present
Liver absent present present present ambiguous ambiguous
Lung absent present present present present present
Mammary Gland absent ambiguous No data absent ambiguous ambiguous
Mesenteric Lymph Node absent present present present present present
Nasal Epithelia absent present present present present present
Oesophagus present present present present present present
Oral Epithelia absent present present present present present
Ovaries No data present No data No data present present
Oviduct No data present No data No data present present
Pancreas present present present present present present
Parathyroid No data No data present present present present
Peripheral Nervous System absent present present present present present
Peyers Patch absent ambiguous present present present present
Pituitary Gland No data No data No data present present No data
Prostate present No data present present No data No data
Skeletal Muscle absent absent absent absent absent absent
Skin present present present present present present
Small Intestine absent present present present present present
Spinal Cord present present present present present present
Spleen absent ambiguous present present present present
Stomach present present present present present present
Testis absent No data present present No data No data
Thymus absent present present present present present
Thyroid No data No data present present present present
Trachea absent present present present present present
Urinary System present present present present present present
Uterus No data present No data No data present present
Vas Deferens absent No data present present No data No data
Vascular System absent absent absent absent absent absent
White Adipose Tissue absent absent absent absent absent absent
Images
  • Tissue: Bladder
    Description: -
    Genotype: Heterozygous
  • Tissue: Brain
    Description: Longitudinal section
    Genotype: Heterozygous
  • Tissue: Brain
    Description: Lateral view
    Genotype: Heterozygous
  • Tissue: Eye
    Description: -
    Genotype: Heterozygous
  • Tissue: Heart
    Description: -
    Genotype: Heterozygous
  • Tissue: Inguinal fat pad
    Description: -
    Genotype: Heterozygous
  • Tissue: Kidney
    Description: Longitudinal section
    Genotype: Heterozygous
  • Tissue: Liver and gall bladder
    Description: -
    Genotype: Heterozygous
  • Tissue: Mesenteric lymph node
    Description: -
    Genotype: Heterozygous
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Heterozygous
  • Tissue: Peyer's patch
    Description: -
    Genotype: Heterozygous
  • Tissue: Skin
    Description: -
    Genotype: Heterozygous
  • Tissue: Skull
    Description: -
    Genotype: Heterozygous
  • Tissue: Stomach
    Description: -
    Genotype: Heterozygous
  • Tissue: Tail
    Description: Cross-section
    Genotype: Heterozygous
  • Tissue: Testis
    Description: -
    Genotype: Heterozygous
Background LacZ Staining in Adult

The adult wholemount LacZ expression protocol has been refined to minimize background staining however, it can still occur. We have systematically documented the sites and intensities of this background stain in male and female wildtype C57BL/6 (C57BL/6J-Tyr<c-Brd>; C57BL/6NTac/Den) mice. The major locations where background staining may be observed are:

  • Nasal cavity
  • Thyroid gland
  • Glandular stomach
  • Kidney
  • Male genitals

Additional background staining is observed occasionally in:

  • Rib cage
  • Thymus
  • Mesenteric lymph nodes
  • Ovaries

Examples of the pattern and level of background stain are presented below.

  • Tissue: Nasal cavity
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Nasal cavity
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Kidney
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Kidney
    Description: -
    Genotype: Wildtype
  • Tissue: Urogenital system
    Description: -
    Genotype: Wildtype
  • Tissue: Testis
    Description: -
    Genotype: Wildtype
  • Tissue: Rib cage
    Description: -
    Genotype: Wildtype
  • Tissue: Thymus
    Description: -
    Genotype: Wildtype
  • Tissue: Mesenteric lymph node
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype