Adult LacZ Expression Data for Cenpj (MBKA)

Overview

Adult expression (≥ 6 weeks): The lacZ reporter gene within the targeting vector was used to determine the wholemount expression profile of the targeted gene. Mice were fixed by cardiac perfusion using 4% paraformaldehyde (PFA). Following dissection, the tissues to be stained were fixed an additional 30 min in 4% PFA before being rinsed in phosphate buffered saline and transferred to lacZ staining solution, containing 0.1% X-gal, for up to 48 hours. After an additional overnight post-staining fixation in 4% PFA, tissues were cleared with 50% glycerol then transferred to 70% glycerol for long-term storage.

Display Criteria

A tick-list of 41 tissues and organs was scored for the presence (green tick) or absence (red cross) of lacZ expression.

  • The term 'Ambiguous' was assigned when there was uncertainty as to the validity of the observed pattern e.g. staining may be very faint, background or an artefact due to trapping.
  • The term 'No Data' was assigned if the stained entity was not available to assess.

By default, up to 27 standard images may be taken using a Leica MZ16A microscope and Imagic software. These images were only collected when X-gal staining was present.

If information or images that you are interested in are not presented, please send details to MGPenquiries@sanger.ac.uk and, on a limited, first come first served basis, we will process your request.

Tick-List
Colony Name Cenpj Knockout First EPD0028_7_G05 Cenpj Knockout First EPD0028_7_G05 Cenpj Knockout First EPD0028_7_G05
Genotype Cenpj/+ Cenpj/+ Cenpj/+
Gender Female Male Female
Age In Weeks 14.8 9.7 9.7
Adrenal Gland absent absent absent
Bone ambiguous absent absent
Brain present present present
Brown Adipose Tissue absent absent absent
Cartilage absent absent absent
Colon absent absent absent
Eye absent absent absent
Gall Bladder No data absent absent
Heart absent absent absent
Kidney ambiguous ambiguous ambiguous
Large Intestine absent absent absent
Liver absent absent absent
Lung absent absent absent
Mammary Gland absent absent absent
Mesenteric Lymph Node absent absent ambiguous
Nasal Epithelia ambiguous absent No data
Oesophagus absent absent absent
Oral Epithelia absent No data absent
Ovaries absent No data ambiguous
Oviduct absent No data absent
Pancreas absent absent absent
Parathyroid No data absent absent
Peripheral Nervous System absent absent absent
Peyers Patch absent absent absent
Pituitary Gland No data ambiguous absent
Prostate No data absent No data
Skeletal Muscle absent absent absent
Skin absent absent absent
Small Intestine absent absent absent
Spinal Cord absent present present
Spleen absent absent absent
Stomach absent absent absent
Testis No data present No data
Thymus absent absent absent
Thyroid No data ambiguous ambiguous
Trachea absent absent absent
Urinary System absent absent absent
Uterus absent No data absent
Vas Deferens No data absent No data
Vascular System absent absent absent
White Adipose Tissue absent absent absent
Images
  • Tissue: Brain
    Description: Longitudinal section
    Genotype: Heterozygous
  • Tissue: Kidney
    Description: -
    Genotype: Heterozygous
  • Tissue: Testis
    Description: -
    Genotype: Heterozygous
Background LacZ Staining in Adult

The adult wholemount LacZ expression protocol has been refined to minimize background staining however, it can still occur. We have systematically documented the sites and intensities of this background stain in male and female wildtype C57BL/6 (C57BL/6J-Tyr<c-Brd>; C57BL/6NTac/Den) mice. The major locations where background staining may be observed are:

  • Nasal cavity
  • Thyroid gland
  • Glandular stomach
  • Kidney
  • Male genitals

Additional background staining is observed occasionally in:

  • Rib cage
  • Thymus
  • Mesenteric lymph nodes
  • Ovaries

Examples of the pattern and level of background stain are presented below.

  • Tissue: Nasal cavity
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Nasal cavity
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Kidney
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Kidney
    Description: -
    Genotype: Wildtype
  • Tissue: Urogenital system
    Description: -
    Genotype: Wildtype
  • Tissue: Testis
    Description: -
    Genotype: Wildtype
  • Tissue: Rib cage
    Description: -
    Genotype: Wildtype
  • Tissue: Thymus
    Description: -
    Genotype: Wildtype
  • Tissue: Mesenteric lymph node
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype