Adult LacZ Expression Data for Abhd5 (MBHQ)

Overview

Adult expression (≥ 6 weeks): The lacZ reporter gene within the targeting vector was used to determine the wholemount expression profile of the targeted gene. Mice were fixed by cardiac perfusion using 4% paraformaldehyde (PFA). Following dissection, the tissues to be stained were fixed an additional 30 min in 4% PFA before being rinsed in phosphate buffered saline and transferred to lacZ staining solution, containing 0.1% X-gal, for up to 48 hours. After an additional overnight post-staining fixation in 4% PFA, tissues were cleared with 50% glycerol then transferred to 70% glycerol for long-term storage.

Display Criteria

A tick-list of 41 tissues and organs was scored for the presence (green tick) or absence (red cross) of lacZ expression.

  • The term 'Ambiguous' was assigned when there was uncertainty as to the validity of the observed pattern e.g. staining may be very faint, background or an artefact due to trapping.
  • The term 'No Data' was assigned if the stained entity was not available to assess.

By default, up to 27 standard images may be taken using a Leica MZ16A microscope and Imagic software. These images were only collected when X-gal staining was present.

If information or images that you are interested in are not presented, please send details to MGPenquiries@sanger.ac.uk and, on a limited, first come first served basis, we will process your request.

Tick-List
Colony Name Abhd5 Knockout First EPD0081_5_E07 Abhd5 Knockout First EPD0081_5_E07 Abhd5 Knockout First EPD0081_5_E07 Abhd5 Knockout First EPD0081_5_E07
Genotype Abhd5/+ Abhd5/+ Abhd5/+ Abhd5/+
Gender Female Male Male Female
Age In Weeks 6.4 6.4 7.7 7.9
Adrenal Gland absent absent absent absent
Bone ambiguous present absent absent
Brain absent ambiguous present ambiguous
Brown Adipose Tissue present present absent absent
Cartilage absent absent absent absent
Colon absent absent absent absent
Eye absent absent absent absent
Gall Bladder No data No data absent absent
Heart present present absent ambiguous
Kidney absent absent absent absent
Large Intestine absent absent absent absent
Liver absent absent absent absent
Lung absent absent absent absent
Mammary Gland absent absent absent absent
Mesenteric Lymph Node absent absent absent absent
Nasal Epithelia ambiguous ambiguous No data No data
Oesophagus present present absent absent
Oral Epithelia ambiguous ambiguous absent absent
Ovaries absent No data No data present
Oviduct absent No data No data absent
Pancreas absent absent absent absent
Parathyroid No data No data absent absent
Peripheral Nervous System absent absent absent absent
Peyers Patch absent absent absent absent
Pituitary Gland No data No data absent No data
Prostate No data absent absent No data
Skeletal Muscle absent absent absent absent
Skin present present present present
Small Intestine absent absent absent absent
Spinal Cord present present ambiguous absent
Spleen absent absent absent absent
Stomach present present absent absent
Testis No data present present No data
Thymus absent absent absent absent
Thyroid No data No data absent absent
Trachea absent present absent absent
Urinary System ambiguous present absent absent
Uterus absent No data No data absent
Vas Deferens No data absent absent No data
Vascular System absent absent absent absent
White Adipose Tissue present present absent absent
Images
  • Tissue: Brown fat pad
    Description: -
    Genotype: Heterozygous
  • Tissue: Heart
    Description: -
    Genotype: Heterozygous
  • Tissue: Inguinal fat pad
    Description: -
    Genotype: Heterozygous
  • Tissue: Rib cage
    Description: -
    Genotype: Heterozygous
  • Tissue: Skin
    Description: -
    Genotype: Heterozygous
  • Tissue: Stomach
    Description: -
    Genotype: Heterozygous
  • Tissue: Tail
    Description: Cross-section
    Genotype: Heterozygous
  • Tissue: Testis
    Description: -
    Genotype: Heterozygous
Background LacZ Staining in Adult

The adult wholemount LacZ expression protocol has been refined to minimize background staining however, it can still occur. We have systematically documented the sites and intensities of this background stain in male and female wildtype C57BL/6 (C57BL/6J-Tyr<c-Brd>; C57BL/6NTac/Den) mice. The major locations where background staining may be observed are:

  • Nasal cavity
  • Thyroid gland
  • Glandular stomach
  • Kidney
  • Male genitals

Additional background staining is observed occasionally in:

  • Rib cage
  • Thymus
  • Mesenteric lymph nodes
  • Ovaries

Examples of the pattern and level of background stain are presented below.

  • Tissue: Nasal cavity
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Nasal cavity
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Kidney
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Kidney
    Description: -
    Genotype: Wildtype
  • Tissue: Urogenital system
    Description: -
    Genotype: Wildtype
  • Tissue: Testis
    Description: -
    Genotype: Wildtype
  • Tissue: Rib cage
    Description: -
    Genotype: Wildtype
  • Tissue: Thymus
    Description: -
    Genotype: Wildtype
  • Tissue: Mesenteric lymph node
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype