Adult LacZ Expression Data for Lrig1 (MBHE)

Overview

Adult expression (≥ 6 weeks): The lacZ reporter gene within the targeting vector was used to determine the wholemount expression profile of the targeted gene. Mice were fixed by cardiac perfusion using 4% paraformaldehyde (PFA). Following dissection, the tissues to be stained were fixed an additional 30 min in 4% PFA before being rinsed in phosphate buffered saline and transferred to lacZ staining solution, containing 0.1% X-gal, for up to 48 hours. After an additional overnight post-staining fixation in 4% PFA, tissues were cleared with 50% glycerol then transferred to 70% glycerol for long-term storage.

Display Criteria

A tick-list of 41 tissues and organs was scored for the presence (green tick) or absence (red cross) of lacZ expression.

  • The term 'Ambiguous' was assigned when there was uncertainty as to the validity of the observed pattern e.g. staining may be very faint, background or an artefact due to trapping.
  • The term 'No Data' was assigned if the stained entity was not available to assess.

By default, up to 27 standard images may be taken using a Leica MZ16A microscope and Imagic software. These images were only collected when X-gal staining was present.

If information or images that you are interested in are not presented, please send details to MGPenquiries@sanger.ac.uk and, on a limited, first come first served basis, we will process your request.

Tick-List
Colony Name Lrig1 Knockout Frist EPD0070_3_G09 Lrig1 Knockout Frist EPD0070_3_G09 Lrig1 Knockout Frist EPD0070_3_G09 Lrig1 Knockout Frist EPD0070_3_G09 Lrig1 Knockout Frist EPD0070_3_G09 Lrig1 Knockout Frist EPD0070_3_G09 Lrig1 Knockout Frist EPD0070_3_G09 Lrig1 Knockout Frist EPD0070_3_G09
Genotype Lrig1/+ Lrig1/+ Lrig1/+ Lrig1/+ Lrig1/+ Lrig1/+ Lrig1/+ Lrig1/+
Gender Female Male Male Male Male Female Female Female
Age In Weeks 3.9 6.5 8.5 8.2 8.2 8.5 8.5 8.4
Adrenal Gland absent absent No data No data No data No data No data No data
Bone absent absent No data No data No data No data No data No data
Brain present present No data No data No data No data No data No data
Brown Adipose Tissue absent present No data No data No data No data No data No data
Cartilage absent absent No data No data No data No data No data No data
Colon present present No data No data No data No data No data No data
Eye present present No data No data No data No data No data No data
Gall Bladder No data present No data No data No data No data No data No data
Heart present present No data No data No data No data No data No data
Kidney absent ambiguous No data No data No data No data No data No data
Large Intestine present present No data No data No data No data No data No data
Liver absent absent No data No data No data No data No data No data
Lung present present No data No data No data No data No data No data
Mammary Gland present present No data No data No data No data No data No data
Mesenteric Lymph Node absent ambiguous No data No data No data No data No data No data
Nasal Epithelia No data No data No data No data No data No data No data No data
Oesophagus absent present No data No data No data No data No data No data
Oral Epithelia absent absent No data No data No data No data No data No data
Ovaries present No data No data No data No data No data No data No data
Oviduct present No data No data No data No data No data No data No data
Pancreas absent absent No data No data No data No data No data No data
Parathyroid No data absent No data No data No data No data No data No data
Peripheral Nervous System present present No data No data No data No data No data No data
Peyers Patch ambiguous ambiguous No data No data No data No data No data No data
Pituitary Gland No data present No data No data No data No data No data No data
Prostate No data absent No data No data No data No data No data No data
Skeletal Muscle absent absent No data No data No data No data No data No data
Skin present present No data No data No data No data No data No data
Small Intestine present present No data No data No data No data No data No data
Spinal Cord present present No data No data No data No data No data No data
Spleen absent absent No data No data No data No data No data No data
Stomach present present No data No data No data No data No data No data
Testis No data present No data No data No data No data No data No data
Thymus ambiguous present No data No data No data No data No data No data
Thyroid No data present No data No data No data No data No data No data
Trachea present present No data No data No data No data No data No data
Urinary System absent absent No data No data No data No data No data No data
Uterus present No data No data No data No data No data No data No data
Vas Deferens No data absent No data No data No data No data No data No data
Vascular System absent absent No data No data No data No data No data No data
White Adipose Tissue absent present No data No data No data No data No data No data
Images
  • Tissue: Brain
    Description: Lateral view
    Genotype: Heterozygous
  • Tissue: Brain
    Description: Longitudinal section
    Genotype: Heterozygous
  • Tissue: Brown fat pad
    Description: -
    Genotype: Heterozygous
  • Tissue: Eye
    Description: -
    Genotype: Heterozygous
  • Tissue: Heart
    Description: -
    Genotype: Heterozygous
  • Tissue: Inguinal fat pad
    Description: -
    Genotype: Heterozygous
  • Tissue: Liver and gall bladder
    Description: -
    Genotype: Heterozygous
  • Tissue: Lung
    Description: -
    Genotype: Heterozygous
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Heterozygous
  • Tissue: Pinna
    Description: -
    Genotype: Heterozygous
  • Tissue: Rib cage
    Description: -
    Genotype: Heterozygous
  • Tissue: Skin
    Description: -
    Genotype: Heterozygous
  • Tissue: Skull
    Description: Internal view
    Genotype: Heterozygous
  • Tissue: Stomach
    Description: -
    Genotype: Heterozygous
  • Tissue: Tail
    Description: -
    Genotype: Heterozygous
  • Tissue: Testis
    Description: -
    Genotype: Heterozygous
  • Tissue: Thymus
    Description: -
    Genotype: Heterozygous
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Heterozygous
Background LacZ Staining in Adult

The adult wholemount LacZ expression protocol has been refined to minimize background staining however, it can still occur. We have systematically documented the sites and intensities of this background stain in male and female wildtype C57BL/6 (C57BL/6J-Tyr<c-Brd>; C57BL/6NTac/Den) mice. The major locations where background staining may be observed are:

  • Nasal cavity
  • Thyroid gland
  • Glandular stomach
  • Kidney
  • Male genitals

Additional background staining is observed occasionally in:

  • Rib cage
  • Thymus
  • Mesenteric lymph nodes
  • Ovaries

Examples of the pattern and level of background stain are presented below.

  • Tissue: Nasal cavity
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Nasal cavity
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Trachea and thyroid
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Stomach
    Description: -
    Genotype: Wildtype
  • Tissue: Kidney
    Description: Longitudinal section
    Genotype: Wildtype
  • Tissue: Kidney
    Description: -
    Genotype: Wildtype
  • Tissue: Urogenital system
    Description: -
    Genotype: Wildtype
  • Tissue: Testis
    Description: -
    Genotype: Wildtype
  • Tissue: Rib cage
    Description: -
    Genotype: Wildtype
  • Tissue: Thymus
    Description: -
    Genotype: Wildtype
  • Tissue: Mesenteric lymph node
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype
  • Tissue: Ovary and Uterus
    Description: -
    Genotype: Wildtype