Auditory Brainstem Response (ABR) measurement for Mcph1tm1a(EUCOMM)WtsiMouse GP

Results Summary

  • ABR thresholds elevated across all frequencies tested
  • Click-ABR waveforms normal

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean threshold is plotted (+/- population standard deviation). The green shaded area indicates the median threshold (and 2.5% to 97.5% reference range) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Superimposed waveforms recorded at 20dB and 50dB sensation level were plotted. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and were plotted as a function of dB sensation level. Means are plotted (over a 95% reference range for each parameter).

ABR Thresholds Summary

ABR Thresholds for Mcph1<tm1a(EUCOMM)Wtsi> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MBGX122.2c M00545149 Mcph1/Mcph1(locked) Female 14.1 55 65 35 45 50 60
MBGX124.1c M00527634 Mcph1/Mcph1(locked) Female 14.2 35 55 30 25 40 45
MBGX124.2d M00541744 Mcph1/Mcph1(locked) Female 14.2 35 50 30 35 40 45
MBGX15.1h M00166290 Mcph1/Mcph1(locked) Female 13.9 35 60 35 35 55 50
MBGX20.1f M00166320 Mcph1/Mcph1(locked) Female 13.9 55 85 50 50 55 55
MBGX48.1g M00213456 Mcph1/Mcph1(locked) Female 14.1 65 85 65 55 55 65
MBGX48.1h M00213457 Mcph1/Mcph1(locked) Female 14.1 30 60 45 50 50 55
MBGX18.1e M00166309 Mcph1/Mcph1(locked) Male 14.1 25 65 45 50 65 70
MBGX18.1f M00166310 Mcph1/Mcph1(locked) Male 14.1 60 85 60 60 55 65
MBGX44.1b M00213415 Mcph1/Mcph1(locked) Male 14.1 35 70 55 55 50 50
MBGX50.1a M00213441 Mcph1/Mcph1(locked) Male 13.9 30 40 20 30 25 35
Mcph1/Mcph1 Average 14.06 41.82 65.45 42.73 44.55 49.09 54.09
Mcph1/Mcph1 Standard Deviation 0.11 13.36 14.21 13.38 10.97 10.18 9.96
Mcph1/Mcph1 Standard Error 0.03 4.03 4.29 4.03 3.31 3.07 3
Controls
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MALC23.1g M00210088 +/+(locked) Female 14.2 25 30 15 25 35 45
MAQX31.1d M00541571 +/+(locked) Female 14.2 20 25 10 15 25 35
MAQX33.1e M00541576 +/+(locked) Female 14.2 20 25 15 20 20 30
MBCL209.1h M00532617 +/+(locked) Female 13.9 25 35 20 20 35 45
MBCL213.1e M00532654 +/+(locked) Female 14.1 20 35 10 30 25 40
MBGX32.1g M00166403 +/+(locked) Female 14.1 15 30 20 25 20 40
MBGX32.1h M00166404 +/+(locked) Female 14.1 25 25 10 20 30 35
MAKR88.1a M00369124 +/+(locked) Male 14.3 25 35 10 15 30 40
MAKR89.1b M00369127 +/+(locked) Male 14.5 30 35 20 25 30 40
MAMV28.1a M00216998 +/+(locked) Male 13.9 25 40 25 25 30 35
MAMV29.1a M00217004 +/+(locked) Male 13.9 20 25 10 20 25 25
MAMV29.1b M00217005 +/+(locked) Male 13.9 25 35 10 20 30 25
MAMV30.1a M00217012 +/+(locked) Male 13.6 25 35 15 25 30 40
MBCB22.1a M00166695 +/+(locked) Male 14.1 20 30 10 10 20 25
MBCB24.1a M00166725 +/+(locked) Male 13.8 20 40 10 25 25 30
MBCB24.1c M00166727 +/+(locked) Male 13.8 20 30 15 20 35 45
MBGX32.1c M00166399 +/+(locked) Male 14.1 25 35 25 20 30 35
MBGX32.1d M00166400 +/+(locked) Male 14.1 25 70 20 35 30 55
MBKM26.1c M00166799 +/+(locked) Male 14.1 25 50 20 30 20 25
MBKV18.1a M00165698 +/+(locked) Male 13.9 25 30 20 15 25 30
MBKV19.1c M00165713 +/+(locked) Male 13.9 25 30 15 20 25 30
MBKV20.1b M00165744 +/+(locked) Male 13.9 20 40 20 25 30 35
MBKV23.1c M00165758 +/+(locked) Male 13.8 25 35 20 30 30 40
+/+ Average 14.02 23.04 34.78 15.87 22.39 27.61 35.87
+/+ Standard Deviation 0.19 3.21 9.5 5.03 5.69 4.63 7.61
+/+ Standard Error 0.04 .67 1.98 1.05 1.19 .97 1.59

Waveform Overlay 20dB SL

Waveform Overlay 50dB SL

Input Output Functions