Auditory Brainstem Response (ABR) measurement for Acsl4tm1a(EUCOMM)WtsiMouse GP

Results Summary

  • ABR thresholds elevated at high frequencies (24-30kHz)
  • Severe Impairment
  • Click ABR waveform shape abnormal
  • Click ABR wave 3 amplitude abnormal
  • Click ABR wave 1 latency abnormal

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean threshold is plotted (+/- population standard deviation). The green shaded area indicates the median threshold (and 2.5% to 97.5% reference range) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Superimposed waveforms recorded at 20dB and 50dB sensation level were plotted. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and were plotted as a function of dB sensation level. Means are plotted (over a 95% reference range for each parameter).

ABR Thresholds Summary

ABR Thresholds for Acsl4<tm1a(EUCOMM)Wtsi> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MBGS55.5g M00934520 Acsl4/+(locked) Female 14.1 30 30 20 35 70 95
MBGS58.4b M00914551 Acsl4/+(locked) Female 14.3 25 25 10 15 25 75
MBGS61.2g M00921340 Acsl4/+(locked) Female 14.1 25 25 15 25 25 55
MBGS61.2h M00921341 Acsl4/+(locked) Female 14.1 30 25 20 10 30 50
MBGS64.3i M00940498 Acsl4/+(locked) Female 13.9 20 40 25 20 25 50
MBGS64.3l M00940501 Acsl4/+(locked) Female 13.9 25 35 20 25 35 95
MBGS65.1d M00921363 Acsl4/+(locked) Female 13.9 30 35 20 20 40 75
Acsl4/+ Average 14.04 26.43 30.71 18.57 21.43 35.71 70.71
Acsl4/+ Standard Deviation 0.14 3.5 5.62 4.4 7.42 14.98 18.21
Acsl4/+ Standard Error 0.05 1.32 2.13 1.66 2.81 5.66 6.88
MBGS29.2a M00280332 Acsl4/Y(locked) Male 14.1 30 45 25 25 60 85
MBGS29.2b M00280333 Acsl4/Y(locked) Male 14.1 30 50 25 25 70 85
MBGS29.2d M00280335 Acsl4/Y(locked) Male 14.1 25 50 15 20 35 90
MBGS29.2f M00280337 Acsl4/Y(locked) Male 14.1 30 35 10 15 35 80
MBGS55.5a M00934514 Acsl4/Y(locked) Male 14.1 35 30 20 30 75 95
MBGS55.5d M00934517 Acsl4/Y(locked) Male 14.1 30 30 15 25 70 85
MBGS55.5e M00934518 Acsl4/Y(locked) Male 14.1 25 30 20 20 80 85
Acsl4/Y Average 14.1 29.29 38.57 18.57 22.86 60.71 86.43
Acsl4/Y Standard Deviation 0.0 3.19 8.75 5.15 4.52 17.2 4.4
Acsl4/Y Standard Error 0.0 1.21 3.31 1.95 1.71 6.5 1.66
Controls
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MBCQ87.2h M00929476 +/+(locked) Female 14.1 25 40 40 30 30 45
MBCQ87.2j M00929478 +/+(locked) Female 14.1 25 35 20 20 25 35
MBCQ94.3c M00921373 +/+(locked) Female 14.1 40 50 30 30 50 55
MBCQ94.3d M00921374 +/+(locked) Female 14.1 25 30 15 20 30 40
MBRK76.3d M00939559 +/+(locked) Female 14.5 20 25 15 15 15 25
MBRK76.3f M00939561 +/+(locked) Female 14.5 25 30 15 20 25 30
MABC55.2b M00277937 +/+(locked) Male 13.6 25 45 35 20 30 35
MABC60.1b M00281317 +/+(locked) Male 13.9 20 25 15 15 20 30
MABC63.1a M00281330 +/+(locked) Male 14.1 25 35 15 10 20 30
MBBZ77.2a M00933303 +/+(locked) Male 14.2 30 30 20 15 30 40
MBBZ77.2b M00933304 +/+(locked) Male 14.2 20 30 5 10 20 35
MBBZ77.2c M00933305 +/+(locked) Male 14.2 25 25 15 20 20 35
MBBZ79.1b M00933324 +/+(locked) Male 13.9 20 25 10 20 30 40
+/+ Average 14.12 25 32.69 19.23 18.85 26.54 36.54
+/+ Standard Deviation 0.23 5.19 7.75 9.58 5.93 8.41 7.44
+/+ Standard Error 0.06 1.44 2.15 2.66 1.65 2.33 2.06

Waveform Overlay 20dB SL

Waveform Overlay 50dB SL

Input Output Functions