Auditory Brainstem Response (ABR) measurement for Atp5a1tm1a(EUCOMM)WtsiMouse GP

Results Summary

  • ABR thresholds normal
  • Click ABR waveform shape abnormal
  • Click ABR wave 3 amplitude abnormal
  • Click ABR wave 3 latency abnormal

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean threshold is plotted (+/- population standard deviation). The green shaded area indicates the median threshold (and 2.5% to 97.5% reference range) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Superimposed waveforms recorded at 20dB and 50dB sensation level were plotted. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and were plotted as a function of dB sensation level. Means are plotted (over a 95% reference range for each parameter).

ABR Thresholds Summary

ABR Thresholds for Atp5a1<tm1a(EUCOMM)Wtsi> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MBCK40.3b M00366776 Atp5a1/+(locked) Female 14.5 25 35 15 10 35 35
MBCK40.3c M00366777 Atp5a1/+(locked) Female 14.5 25 30 15 20 30 35
MBCK47.1h M00371902 Atp5a1/+(locked) Female 13.8 30 55 35 30 35 50
MBCK47.1i M00371903 Atp5a1/+(locked) Female 13.8 25 30 10 25 30 40
MBCK51.1b M00426554 Atp5a1/+(locked) Female 14.2 25 30 15 20 25 35
MBCK53.1c M00426566 Atp5a1/+(locked) Female 13.9 25 30 10 20 35 45
MBCK53.1d M00426567 Atp5a1/+(locked) Female 13.9 25 30 15 20 25 35
MBCK54.2a M00493006 Atp5a1/+(locked) Male 13.8 25 30 10 20 25 30
MBCK54.2b M00493007 Atp5a1/+(locked) Male 13.8 25 40 25 25 25 35
MBCK61.1a M00528793 Atp5a1/+(locked) Male 14.1 25 40 15 30 35 35
MBCK61.1b M00528794 Atp5a1/+(locked) Male 14.1 25 35 15 15 30 40
Atp5a1/+ Average 14.04 25.45 35 16.36 21.36 30 37.73
Atp5a1/+ Standard Deviation 0.26 1.44 7.39 7.1 5.68 4.26 5.38
Atp5a1/+ Standard Error 0.08 .43 2.23 2.14 1.71 1.29 1.62
Controls
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MAKT46.4e M00363924 +/+(locked) Female 14.1 30 40 25 20 40 35
MAKT46.4g M00368352 +/+(locked) Female 14.1 25 30 15 25 35 35
MBEQ54.1f M00425103 +/+(locked) Female 14.1 20 30 10 20 25 35
MBEQ54.1h M00425105 +/+(locked) Female 14.1 20 40 20 10 30 30
MAKT49.3d M00363888 +/+(locked) Male 14.2 25 35 10 25 35 40
MAKT49.3f M00363890 +/+(locked) Male 14.2 25 35 20 20 30 25
MBCL209.1b M00532611 +/+(locked) Male 13.9 30 40 30 30 30 30
MBCL213.1b M00532651 +/+(locked) Male 14.1 20 25 10 15 25 25
MBKW63.1a M00485910 +/+(locked) Male 14.2 25 45 20 25 30 35
MBKW63.1b M00485911 +/+(locked) Male 14.2 25 25 15 20 35 30
+/+ Average 14.12 24.5 34.5 17.5 21 31.5 32
+/+ Standard Deviation 0.09 3.5 6.5 6.42 5.39 4.5 4.58
+/+ Standard Error 0.03 1.11 2.06 2.03 1.7 1.42 1.45

Waveform Overlay 20dB SL

Waveform Overlay 50dB SL

Input Output Functions