Auditory Brainstem Response (ABR) measurement for Cldn16tm1a(KOMP)WtsiMouse GP

Results Summary

  • ABR thresholds normal
  • Click-ABR waveforms normal

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean threshold is plotted (+/- population standard deviation). The green shaded area indicates the median threshold (and 2.5% to 97.5% reference range) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Superimposed waveforms recorded at 20dB and 50dB sensation level were plotted. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and were plotted as a function of dB sensation level. Means are plotted (over a 95% reference range for each parameter).

ABR Thresholds Summary

ABR Thresholds for Cldn16<tm1a(KOMP)Wtsi> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MAPG16.2a M00439446 Cldn16/Cldn16(locked) Male 14.1 15 30 10 15 20 25
MAPG16.2d M00439449 Cldn16/Cldn16(locked) Male 14.1 20 30 20 15 20 30
MAPG16.2f M00439451 Cldn16/Cldn16(locked) Male 14.1 20 25 10 15 20 25
MAPG21.1a M00433447 Cldn16/Cldn16(locked) Male 14.2 20 30 15 15 25 30
MAPG21.1b M00433448 Cldn16/Cldn16(locked) Male 14.2 20 35 10 10 15 30
MAPG21.1c M00433449 Cldn16/Cldn16(locked) Male 14.2 20 30 15 10 35 40
MAPG21.1d M00433450 Cldn16/Cldn16(locked) Male 14.2 20 25 10 15 25 35
Cldn16/Cldn16 Average 14.16 19.29 29.29 12.86 13.57 22.86 30.71
Cldn16/Cldn16 Standard Deviation 0.05 1.75 3.19 3.64 2.26 5.89 4.95
Cldn16/Cldn16 Standard Error 0.02 .66 1.21 1.38 .85 2.23 1.87
Controls
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MBTN28.1e M00468780 +/+(locked) Female 13.5 25 40 20 20 30 35
MBTN28.1f M00468781 +/+(locked) Female 13.5 25 30 25 25 30 40
MBFA52.2a M00430102 +/+(locked) Male 14.2 20 25 5 10 20 25
MBFA52.2c M00430104 +/+(locked) Male 14.2 20 25 15 20 25 25
MBKQ62.2a M00438191 +/+(locked) Male 14.3 15 30 5 15 30 35
MBKQ65.2b M00438199 +/+(locked) Male 14.1 20 30 15 20 25 30
+/+ Average 13.97 20.83 30 14.17 18.33 26.67 31.67
+/+ Standard Deviation 0.33 3.44 5 7.31 4.71 3.73 5.53
+/+ Standard Error 0.14 1.4 2.04 2.99 1.92 1.52 2.26