Indirect Calorimetry Data for Dlg4 (MAOR)
Standard Protocol - significant parameters
Indirect Calorimetry (week 12, male mice prioritised) [Mice were fed on High Fat Diet (21.4% crude fat content, Western RD, 829100, Special Diets Services) from 4 weeks of age]: Respiratory metabolic function is assessed using the LabMaster system (TSE-systems). Mice are housed individually in calorimetry cages for a period of approximately 21 hours including a 5 hour light phase acclimatisation period. This is followed consecutively by a 12 hour dark (19:30 - 07:30) and minimum 3 hour light phase. Cumulative food intake, activity (beam breaks), volume of oxygen consumed and volume of carbon dioxide produced are all measured. From this data the respiratory exchange ratio and energy expenditure are derived. Water bottles are weighed before and after the experiment to assess water consumption.
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| Legend - hover over each heading to get a more complete description | ||||||||
| Test complete and data/resources are available | Test is complete and the data are considered interesting | Preliminary indication of an interesting phenotype | ||||||
| Test is complete but the data are not considered interesting | Test not performed or applicable | Test pending | ||||||
| Test abandoned | ||||||||
| Parameter | Female Hom |
Male Hom |
population parameter |
Graph | MP Annotation |
|---|---|---|---|---|---|
| Cumulative Food Intake | |||||
| Water Intake | |||||
| Respiratory Exchange Ratio | view graph | MP:0010378 - increased respiratory quotient | |||
| VCO2 | view graph | MP:0008963 - increased carbon dioxide production | |||
| VO2 | view graph | MP:0005289 - increased oxygen consumption | |||
| Energy Expenditure | view graph | MP:0004889 - increased energy expenditure | |||
| Energy Expenditure/Animal | |||||
| X-Activity Total | |||||
| X-Activity Ambulatory |
