Auditory Brainstem Response (ABR) measurement for Hmx3hx MGP Pipeline 2 (Hmx3 Generic Baseline)

Results Summary

  • ABR thresholds normal
  • Click-ABR waveforms normal

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean threshold is plotted (+/- population standard deviation). The green shaded area indicates the median threshold (and 2.5% to 97.5% reference range) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Superimposed waveforms recorded at 20dB and 50dB sensation level were plotted. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and were plotted as a function of dB sensation level. Means are plotted (over a 95% reference range for each parameter).

ABR Thresholds Summary

ABR Thresholds for Hmx3<hx> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MAMM15.2c M00197947 hx/hx(locked) Female 12.9 25 35 15 20 20 35
MAMM15.2f M00197950 hx/hx(locked) Female 12.9 25 40 10 20 20 40
MAMM16.2d M00197957 hx/hx(locked) Female 13.2 35 50 15 20 25 45
MAMM21.1c M00153602 hx/hx(locked) Female 13.1 45 50 30 25 35 50
MAMM39.2i M00153669 hx/hx(locked) Female 13.8 30 75 35 30 25 55
MAMM43.2d M00197975 hx/hx(locked) Male 13.1 15 20 10 15 25 40
MAMM50.1a M00197983 hx/hx(locked) Male 12.9 30 45 20 30 30 45
MAMM58.2b M00198006 hx/hx(locked) Male 12.9 25 60 35 15 30 40
hx/hx Average 13.1 28.75 46.88 21.25 21.88 26.25 43.75
hx/hx Standard Deviation 0.29 8.2 15.4 9.92 5.56 4.84 5.99
hx/hx Standard Error 0.1 2.9 5.44 3.51 1.96 1.71 2.12
Controls
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MAMM15.2d M00197948 +/+(locked) Female 12.9 20 35 20 20 30 85
MAMM18.2d M00197961 +/+(locked) Female 13.1 25 35 20 20 35 85
MAMM22.1f M00153630 +/+(locked) Female 13.2 30 40 25 20 55 85
MAMM38.1d M00153649 +/+(locked) Female 13.1 35 60 30 35 45 85
MAMM55.1d M00153726 +/+(locked) Female 13.1 25 35 15 20 35 80
MAMM59.1e M00198022 +/+(locked) Female 13.1 30 30 20 15 30 55
MAMM16.2b M00197955 +/+(locked) Male 13.2 20 35 20 20 25 80
MAMM51.2a M00197995 +/+(locked) Male 13.2 25 35 25 15 30 70
MAMM58.2c M00198007 +/+(locked) Male 12.9 25 35 10 15 30 45
+/+ Average 13.09 26.11 37.78 20.56 20 35 74.44
+/+ Standard Deviation 0.11 4.58 8.2 5.5 5.77 8.82 14.03
+/+ Standard Error 0.04 1.53 2.73 1.83 1.92 2.94 4.68