Auditory Brainstem Response (ABR) measurement for Myo6sv MGP Pipeline 2 (Myo6 Generic Baseline)

Results Summary

  • ABR thresholds elevated across all frequencies tested
  • Severe Impairment
  • Tested as a positive control. Deafness already known

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean threshold is plotted (+/- population standard deviation). The green shaded area indicates the median threshold (and 2.5% to 97.5% reference range) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Superimposed waveforms recorded at 20dB and 50dB sensation level were plotted. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and were plotted as a function of dB sensation level. Means are plotted (over a 95% reference range for each parameter).

ABR Thresholds Summary

ABR Thresholds for Myo6<sv> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MAMJ19.1d M00056497 +/sv(locked) Female 13.5 22 31 13 16 25 43
MAMJ19.1e M00056498 +/sv(locked) Female 13.5 28 31 19 25 34 46
MAMJ17.1a M00056473 +/sv(locked) Male 13.4 25 34 22 22 34 49
MAMJ17.1b M00056474 +/sv(locked) Male 13.4 25 37 19 19 28 43
MAMJ17.1c M00056475 +/sv(locked) Male 13.4 22 34 19 19 28 40
+/sv Average 13.44 24.4 33.4 18.4 20.2 29.8 44.2
+/sv Standard Deviation 0.05 2.24 2.24 2.94 3.06 3.6 3.06
+/sv Standard Error 0.02 1 1 1.31 1.37 1.61 1.37
MAMJ17.1d M00056476 sv/sv(locked) Female 13.4 85 85 85 85 85 85
MAMJ17.1e M00056477 sv/sv(locked) Female 13.4 85 85 85 85 85 85
MAMJ2.5f M00057082 sv/sv(locked) Female 13.3 85 85 85 85 85 85
MAMJ14.1a M00056437 sv/sv(locked) Male 13.4 85 85 85 85 85 85
MAMJ14.1b M00056438 sv/sv(locked) Male 13.4 85 85 85 85 85 85
MAMJ14.1d M00056440 sv/sv(locked) Male 13.4 85 85 85 85 85 76
sv/sv Average 13.38 85 85 85 85 85 83.5
sv/sv Standard Deviation 0.04 0 0 0 0 0 3.35
sv/sv Standard Error 0.02 0 0 0 0 0 1.37