Auditory Brainstem Response (ABR) measurement for Chd7WhiMouse GP

Results Summary

  • ABR thresholds elevated across all frequencies tested
  • Moderate Impairment

Methods Summary

  • Auditory Brainstem Responses (ABRs) are recorded via subcutaneous needle electrodes placed at the vertex and behind the left ear, as an average of 256 responses to presentation of freefield stimuli [clicks (10us) or tone pips (5ms tone pips, 1ms rise/fall time)], presented at 42.2/sec.
  • ABR Thresholds are determined and plotted as a function of stimulus. Mean threshold is plotted (+/- population standard deviation). The green shaded area indicates the median threshold (and 2.5% to 97.5% reference range) of a large population of wildtype mice of this genetic background.
  • Click-Evoked ABRs waveforms were analysed. Superimposed waveforms recorded at 20dB and 50dB sensation level were plotted. Amplitudes and Latencies for waves P1, N1, P3 and N3 were recorded and were plotted as a function of dB sensation level. Means are plotted (over a 95% reference range for each parameter).

ABR Thresholds Summary

ABR Thresholds for Chd7<Whi> mutants and controls

ABR Thresholds and Waveforms

Subjects
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MAMF14.1d M00189579 Whi/+(locked) Female 14.2 30 75 45 35 40 70
MAMF14.1e M00189580 Whi/+(locked) Female 14.2 25 65 40 45 45 75
MAMF14.1b M00189577 Whi/+(locked) Male 14.2 35 60 40 55 60 65
MAMF14.1c M00189578 Whi/+(locked) Male 14.2 30 60 25 50 40 40
Whi/+ Average 14.2 30 65 37.5 46.25 46.25 62.5
Whi/+ Standard Deviation 0.0 3.54 6.12 7.5 7.4 8.2 13.46
Whi/+ Standard Error 0.0 1.77 3.06 3.75 3.7 4.1 6.73
Controls
Mouse Name Mouse Barcode Genotype Gender Age click 6kHz 12kHz 18kHz 24kHz 30kHz
MAMF8.1d M00189555 +/+(locked) Female 14.1 20 35 10 20 25 30
MAMF8.1e M00189556 +/+(locked) Female 14.1 20 30 10 15 20 35
MAMF11.1b M00189571 +/+(locked) Male 14.1 25 35 25 25 30 35
MAMF14.1a M00189576 +/+(locked) Male 14.2 20 35 20 25 30 40
+/+ Average 14.13 21.25 33.75 16.25 21.25 26.25 35
+/+ Standard Deviation 0.04 2.17 2.17 6.5 4.15 4.15 3.54
+/+ Standard Error 0.02 1.08 1.08 3.25 2.07 2.07 1.77